A5032409985- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Mycotoxins in Agriculture and Food
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogenic Bacteria Studies
- Phytoplasmas and Hemiptera pathogens
- Fungal and yeast genetics research
- Yeasts and Rust Fungi Studies
- Bioenergy crop production and management
- Genetic Mapping and Diversity in Plants and Animals
- Biofuel production and bioconversion
- Nematode management and characterization studies
- Sugarcane Cultivation and Processing
- Plant Pathogens and Resistance
- Fungal Biology and Applications
- Cocoa and Sweet Potato Agronomy
- Wheat and Barley Genetics and Pathology
- Rice Cultivation and Yield Improvement
- Natural Products and Biological Research
- Genetic diversity and population structure
- Turfgrass Adaptation and Management
- Plant responses to water stress
United States Department of Agriculture
2015-2024
Animal and Plant Health Inspection Service
2017-2024
Agricultural Research Service
2014-2023
Texas A&M University
2013
Rutgers, The State University of New Jersey
2013
Rapid and accurate detection of plant pathogens in the field is crucial to prevent proliferation infected crops. Polymerase chain reaction (PCR) process most reliable accepted method for pathogen diagnosis, however current conventional PCR machines are not portable require additional post-processing steps detect amplified DNA (amplicon) pathogens. Real-time can directly quantify amplicon during amplification without need post processing, thus more suitable operations, still takes time large...
Abstract High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in sample. The growing interest application HTS technologies for routine diagnostics plant health laboratories triggering development guidelines on how to prepare performing testing. This paper describes general technical recommendations guide through complex process preparing laboratory tests within existing quality assurance systems....
Early and accurate diagnosis of new plant pathogens is vital for the rapid implementation effective mitigation strategies appropriate regulatory responses. Most commonly, pathogen identification relies on morphology DNA marker analysis. However, diseases, these approaches may not be sufficient precise diagnosis. In this study, we used whole-genome sequencing (WGS) to identify causal agent a disease affecting Sarcococca hookeriana (sarcococca). Blight symptoms were observed sarcococca...
The genomes of Chrysoporthe austroafricana, Diplodia scrobiculata, Fusarium nygami, Leptographium lundbergii, Limonomyces culmigenus, Stagonosporopsis tanaceti, and Thielaviopsis punctulata are presented in this genome announcement. These seven from endophytes, plant pathogens economically important fungal species. sizes range 26.6 Mb the case lundbergii to 44 for austroafricana. availability these data will provide opportunities resolve longstanding questions regarding taxonomy species...
Abstract Rapid and accurate molecular diagnostic tools are critical to efforts minimize the impact spread of emergent pathogens. The identification markers for novel pathogens presents several challenges, especially in absence information about population diversity where genetic resources limited. objective this study was use comparative genomics datasets find unique target regions suitable diagnosis two fungal species causing a newly blight disease boxwood. Candidate marker loop-mediated...
Dactylonectria macrodidyma is part of the Nectriaceae, a family containing important plant pathogens. This species possesses ability to induce disease on grapevine, avocado, and olive. Here, we report first draft genome D. isolate JAC15-245. The assembled was 58 Mbp contained an estimated 16,454 genes.
Rapid global germplasm trade has increased concern about the spread of plant pathogens and pests across borders that could become established, affecting agriculture environment systems. Viral are particular due to their difficulty control once established. A comprehensive diagnostic platform accurately detects both known unknown virus species, as well unreported variants, is playing a pivotal role quarantine programs. Here we propose addition high-throughput sequencing (HTS) from total RNA...
Boxwood (Buxus spp.) are commercially important evergreen ornamental plants with an annual market value of over $103 million in the United States. The recent U.S. incursion boxwood blight disease caused by fungus Calonectria pseudonaviculata (syn. Cylindrocladium pseudonaviculatum, Cy. buxicola) threatens health and productivity both landscape plantings nurseries. first confirmed reports were made from Connecticut North Carolina November 2011 (2,4), followed diagnoses 10 additional states...
Sugarcane yellow leaf virus (SCYLV), the causal agent of leaf, has been reported in an increasing number sugarcane-growing locations since its first report 1990s Brazil, Florida, and Hawaii. In this study, genetic diversity SCYLV was investigated using genome coding sequence (5,561 to 5,612 nt) 109 isolates from 19 geographical locations, including 65 new 16 regions worldwide. These were distributed three major phylogenetic lineages (BRA, CUB, REU), except for one isolate Guatemala....
Summary F usarium verticillioides is a pathogen of maize causing ear rot and stalk rot. The fungus also produces fumonisins, group mycotoxins linked to disorders in animals humans. A cluster genes, designated FUM plays key role the synthesis fumonisins. However, our understanding regulatory mechanism fumonisin biosynthesis still incomplete. We have demonstrated previously that C pp1, protein phosphatase type 2A ( PP2A ) catalytic subunit, negatively regulates production involved cell shape...
Boxwood (Buxus spp.) are broad-leaved, evergreen landscape plants valued for their longevity and ornamental qualities. Volutella leaf stem blight, caused by the ascomycete fungi Pseudonectria foliicola P. buxi, is one of major diseases affecting health qualities boxwood. Although this disease less severe than boxwood blight Calonectria pseudonaviculata C. henricotiae, its widespread occurrence disfiguring symptoms have substantial economic losses to industry. In study, we sequenced genome...
Plant pathogens cause significant reductions in yield and crop quality enormous economic losses worldwide. Reducing these provides an obvious strategy to increase food production without further degrading natural ecosystems; however, this requires knowledge of the biology evolution agroecosystems.
The ubiquitous ascomycete Fusarium verticillioides causes ear rot and stalk of maize, both which reduce grain quality yield. Additionally, F. produces the mycotoxin fumonisin B1 (FB1) during infection maize kernels, thus potentially compromises human animal health. current knowledge is fragmentary regarding regulation FB1 biosynthesis, particularly when considering interplay with environmental factors such as nutrient availability. In this study, SDA1 verticillioides, predicted to encode a...
• Premise of the study: Genic microsatellites or simple sequence repeat (genic‐SSR) markers were developed in boxwood ( Buxus taxa) for genetic diversity analysis, identification taxa, and to facilitate breeding. Methods Results: cDNA libraries from mRNA extracted leaves sempervirens ‘Vardar Valley’ sequenced using Illumina MiSeq system. Approximately 11.9 million base pairs data examined 845 genic‐SSRs identified, including 469 dinucleotide, 360 trinucleotide, seven tetranucleotide, one...
Abstract High throughput sequencing (HTS) can supplement and may replace diagnostic tests for plant pathogens. However, the methodology processing of HTS data must first be optimized standardized to ensure sensitivity repeatability results. Importation sugarcane into United States is highly regulated, plants are subjected strict quarantine measures testing, especially presence certain viruses regulatory concern. Here, we tested whether could reliably detect four RNA three DNA over seasons...
Fungi in the genus Clarireedia are widespread and destructive pathogens of grasses worldwide, best known as causal agents dollar spot disease turfgrass. Here, we report genome assemblies seven isolates, including ex-types two most species, jacksonii C. monteithiana. These datasets provide a valuable resource for ongoing studies that include population diversity, host–pathogen interactions, marker development, control.
Miscanthus sinensis is a grass used for sugarcane breeding and bioenergy production. Using high throughput sequencing technologies, we identified new viral genome in infected M. leaf tissue displaying yellow fleck symptoms. This virus most related to members of the genus Polerovirus family Luteoviridae. The canonical ORFs were computationally identified, P3 coat protein was expressed, virus-like particles purified found conform icosahedral shapes, characteristic We propose name this virus.