A5081148533- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Resistance
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Fungal Diseases
- Cocoa and Sweet Potato Agronomy
- Plant Parasitism and Resistance
- Bacteriophages and microbial interactions
- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Plant Disease Management Techniques
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Animal Virus Infections Studies
- Chromosomal and Genetic Variations
- Entomopathogenic Microorganisms in Pest Control
- Mosquito-borne diseases and control
- Insect behavior and control techniques
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Forest Insect Ecology and Management
Fera Science (United Kingdom)
2016-2025
Newcastle University
2022-2024
Eastern Health
2014
Scottish Agricultural Science Agency
2005
Central Electricity Generating Board
1989
Next generation sequencing (NGS) technologies are becoming routinely employed in different fields of virus research. Different platforms and sample preparation approaches, the laboratories worldwide, contributed to a revolution detection discovery plant viruses viroids. In this work, we presenting comparison two RNA sequence inputs (small RNAs versus ribosomal depleted total RNA) for by Illumina sequencing. This includes several viruses, which differ genome organization viroids from both...
OPINION article Front. Plant Sci., 27 August 2018Sec. Virology Volume 9 - 2018 | https://doi.org/10.3389/fpls.2018.01082
The diagnosis of novel unidentified viral plant diseases can be problematic, as the conventional methods such real‐time PCR or ELISA may too specific to a particular species even strain virus, whilst alternatives electron microscopy ( EM ) sap inoculation indicator do not usually give level diagnosis. Next‐generation sequencing NGS offers an alternative solution where sequence is generated in non‐specific fashion and identification based on similarity searching against G en B ank. techniques...
Abstract The discipline of plant pathology has an expanding remit requiring a multi-faceted, interdisciplinary approach to capture the complexity interactions for any given disease, disease complex or syndrome. This review discussed recent developments in research and identifies some key issues that, we anticipate, must be faced meet food security environmental challenges that will arise over coming decades. In meeting these issues, challenge turn is community respond by contributing wider...
Maize lethal necrosis (MLN) is a disease of maize caused by the combination chlorotic mottle virus (MCMV) and potyvirus (Uyemote et al., 6). MLN MCMV Sugarcane mosaic (SCMV) was identified in Kenya 2012 (Adams 1) associated with significant damage. Subsequent to outbreak Kenya, symptoms have been SCMV Tanzania Uganda and, based on our initial findings, but not Rwanda (ASARECA, 2). The control priority for East Africa region. We report here fuller investigation causal agents from Rwanda. In...
Internal necrosis of carrot has been observed in UK carrots for at least 10 years, and anecdotally linked to virus infection. In the 2009 growing season some growers had up 10% yield with these symptoms. Traditional diagnostic methods are targeted towards specific pathogens. By using a metagenomic approach high throughput sequencing technology, other, as yet unidentified causes root were investigated. Additionally statistical analysis shown which viruses most closely associated disease...
In July 2019 a sample of tomato leaves (Solanum lycopersicon cv. Piccolo) was submitted to Fera Science Ltd, from grower in the South England. The sent following appearance mosaic symptoms, which had spread throughout crop affected glasshouse. Symptoms were consistent with viral infection and emerged inoculation mild strain Pepino virus (PepMV) as cross-protection treatment, however symptoms persisted post-inoculation. tested using ELISA kits for presence PepMV (Bioreba, Switzerland) plus...
Abstract High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in sample. The growing interest application HTS technologies for routine diagnostics plant health laboratories triggering development guidelines on how to prepare performing testing. This paper describes general technical recommendations guide through complex process preparing laboratory tests within existing quality assurance systems....
High-throughput sequencing (HTS) technologies have the potential to become one of most significant advances in molecular diagnostics. Their use by researchers detect and characterize plant pathogens pests has been growing steadily for more than a decade they are now envisioned as routine diagnostic test be deployed pest diagnostics laboratories. Nevertheless, HTS downstream bioinformatics analysis generated datasets represent complex process including many steps whose reliability must...
High-throughput sequencing (HTS) has become an important tool for plant virus detection and discovery. Nanopore been rapidly developing in the recent years offers new possibilities fast diagnostic applications of HTS. With this mind, a study was completed, comparing most established HTS platform (MiSeq benchtop sequencer-Illumina), with MinION sequencer (Oxford Technologies) viruses viroids. Method comparisons were performed on five selected samples, containing two viroids, which sequenced...
Tomato brown rugose fruit virus (ToBRFV) has recently emerged as a major disease of tomatoes and peppers. ToBRFV is seed- contact-transmitted virus. In Slovenia, RNA was detected in samples wastewater, river, water used to irrigate plants. Even though the source could not be clearly established, this raised question significance detection experimental studies were performed address question. The data presented here confirm that release particles from roots infected plants infectious can...
There is only limited knowledge of the presence and incidence viruses in peas within United Kingdom, therefore high-throughput sequencing (HTS) combination with a bulk sampling strategy targeted testing was used to determine virome cultivated pea crops. Bulks 120 leaves collected from twenty fields around UK were initially tested by HTS, virus then determined using specific real-time reverse-transcription PCR assays smaller mixed-bulk size samples. This study presents first finding turnip...
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The advances in high-throughput sequencing (HTS) technologies and bioinformatic tools have provided new opportunities for virus viroid discovery diagnostics. Hence, sequences of viral origin are being discovered published at a previously unseen rate. Therefore, collective effort was undertaken to write propose framework prioritizing the biological characterization steps needed after discovering plant evaluate its impact different levels. Even though proposed approach widely used, revision...
Ullucus tuberosus (ulluco) is a tuber‐forming species that has become novel crop in highland and temperate maritime climates. Eight viruses have been previously reported infecting , including Andean potato latent virus ( APLV ), quarantine within the European Union. No reference sequences published for described from U . Plants grown UK internet trade were tested presence of using ELISA real‐time RT ‐ PCR positive results obtained multiple other viruses. A similar suite was detected at...
The aphid‐transmitted viruses Potato virus Y ( PVY ) and A PVA commonly affect seed potatoes in the UK . transmission efficiency for aphid species is used to calculate a potential risk expressed as relative factor REF ). These s have not previously been calculated strains of or clones. Using published method, occurring potatoes. Myzus persicae nominally set 1 other are this. data represent first transmission. Macrosiphum euphorbiae 0.91) was almost efficient M. at were further analysed...
Forty-seven potato virus A (PVA) isolates from Europe, Australia, and South America’s Andean region were subjected to high-throughput sequencing, 46 complete genomes Europe (n = 9), Australia 2), the Andes 35) obtained. These 17 other gave alignments of 63 open reading frames 9,180 nucleotides long; 9 recombinants. The nonrecombinants formed three tightly clustered, almost equidistant phylogroups; comprised 14 Peruvian isolates; W 37 in Peru, Argentina, elsewhere world; T contained tamarillo...
Abstract Plantain virus X was first recognized by the ICTV as a species in genus Potexvirus 1982. However, because no sequence available for plantain (PlVX), abolishing proposed to Flexiviridae working group of 2015. This initiated efforts original isolates from Plantago lanceolata samples. Here we report full‐genome sequencing two PlVX, which demonstrate that is synonymous Actinidia , previously reported kiwifruit ( sp.) and blackcurrant Ribes nigrum ). PlVX noted be widespread UK P ....