A5071183460- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Turfgrass Adaptation and Management
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Resistance
- Wheat and Barley Genetics and Pathology
- Fungal Biology and Applications
- Fungal Plant Pathogen Control
- Fermentation and Sensory Analysis
- Yeasts and Rust Fungi Studies
- Genomics and Phylogenetic Studies
- Antifungal resistance and susceptibility
- Biofuel production and bioconversion
- Genetic Mapping and Diversity in Plants and Animals
- Meat and Animal Product Quality
- Plant pathogens and resistance mechanisms
- Plant Pathogenic Bacteria Studies
- Plant and Fungal Interactions Research
- RNA and protein synthesis mechanisms
- Advanced Chemical Sensor Technologies
- Protist diversity and phylogeny
- Fungal Infections and Studies
The Ohio State University
2012-2022
Chinese Academy of Agricultural Sciences
2017
Institute of Plant Protection
2017
Sokoine University of Agriculture
2016-2017
University of Arkansas at Fayetteville
2016-2017
International Livestock Research Institute
2017
International Rice Research Institute
2017
University of Exeter
2016-2017
University of the Philippines Los Baños
2017
Kasetsart University
2017
Magnaporthe grisea is the most destructive pathogen of rice worldwide and principal model organism for elucidating molecular basis fungal disease plants. Here, we report draft sequence M. genome. Analysis gene set provides an insight into adaptations required by a fungus to cause disease. The genome encodes large diverse secreted proteins, including those defined unusual carbohydrate-binding domains. This also possesses expanded family G-protein-coupled receptors, several new...
The filamentous fungus Trichoderma reesei produces and secretes profuse quantities of enzymes that act synergistically to degrade cellulase related biomass components. We partially sequenced over 5100 random T. cDNA clones. Among the sequences whose predicted gene products had significant similarity known proteins, 12 were identified encode previously unknown likely function in degradation. Microarrays used query expression levels each under different conditions induce cellulolytic enzyme...
Genetic analysis of disease emergence In the 1980s, wheat crops began to fall fungal pathogen that causes blast disease. First seen in Brazil, last year caused devastating crop losses Bangladesh. Inoue et al. tracked down shifting genetics have allowed this potentially global threat (see Perspective by Maekawa and Schulze-Lefert). Wheat varieties with a disabled resistance gene were susceptible strains affected oat ryegrass crops. Subsequent genetic changes amped up virulence wheat. Science...
Delineating species and epidemic lineages in fungal plant pathogens is critical to our understanding of disease emergence the structure biodiversity also informs international regulatory decisions.
The jasmonate family of phytohormones plays central roles in plant development and stress acclimation. However, the architecture their signaling circuits remains largely unknown. Here we describe a binding protein, cyclophilin 20-3 (CYP20-3), which regulates stress-responsive cellular redox homeostasis. (+)-12-oxo-phytodienoic acid (OPDA) promotes CYP20-3 to form complex with serine acetyltransferase 1, triggers formation hetero-oligomeric cysteine synthase O -acetylserine(thiol)lyase B...
Magnaporthe grisea, the causal agent of rice blast disease, differentiates a specialized infection cell, an appressorium, that is required for its host. Previously, cAMP was implicated in endogenous signaling pathway leading to appressorium formation. To obtain direct evidence role formation, gene encoding catalytic subunit cAMP-dependent protein kinase (cpkA) cloned, sequenced, and disrupted. Polymerase chain reaction primers designed after highly conserved regions same from other organisms...
Abstract Background Rice blast disease is caused by the filamentous Ascomycetous fungus Magnaporthe oryzae and results in significant annual rice yield losses worldwide. Infection this many other fungal plant pathogens requires development of a specialized infection cell called an appressorium. The molecular processes regulating appressorium formation are incompletely understood. Results We analyzed genome-wide gene expression changes during spore germination on hydrophobic surface compared...
Background and objective: Asthma allergic rhinitis are significant, increasing causes of morbidity worldwide. Pollen, a major cause seasonal rhinitis/conjunctivitis, carries proteolytic enzymes on its surface. We showed previously that peptidase allergens from house dust mites compromise epithelial barrier function by degrading the extracellular domains tight junction proteins, occludin claudin, thus facilitating allergen delivery across layers. In this study, we aimed to determine whether...
The Aspergillus fumigatus sterol regulatory element binding protein (SREBP) SrbA belongs to the basic Helix-Loop-Helix (bHLH) family of transcription factors and is crucial for antifungal drug resistance virulence. latter phenotype especially striking, as loss results in complete virulence murine models invasive pulmonary aspergillosis (IPA). How fungal SREBPs mediate unknown, though it has been suggested that lack growth hypoxic conditions accounts attenuated To further understand role...
Interactions between rice and Magnaporthe oryzae involve the recognition of cellular components exchange complex molecular signals from both partners. How these interactions occur in cells is still elusive. We employed robust-long serial analysis gene expression, massively parallel signature sequencing, sequencing by synthesis to examine transcriptome profiles infected leaves. A total 6,413 planta–expressed fungal genes, including 851 genes encoding predicted effector proteins, were...
Secreted proteins play central roles in plant-microbe interactions acting as signals, toxins, and effectors. One important group of small secreted is the snodprot1 family, members which have demonstrated phytotoxic properties. A split-marker transformation system was applied for gene deletion homolog, MSP1, rice blast fungus Magnaporthe grisea. msp1 mutants were phenotypically indistinguishable from wild type elaborated apparently normal appressoria. However, greatly reduced virulence...
Abstract Background Emerging knowledge of the impact small RNAs as important cellular regulators has prompted an explosion transcriptome sequencing projects. Although significant progress been made towards RNA discovery and biogenesis in higher eukaryotes other model organisms, simple such filamentous fungi remains limited. Results Here, we used 454 pyrosequencing to present a detailed analysis (~ 15 - 40 nucleotides length) from mycelia appressoria tissues rice blast fungal pathogen,...
Summary Alternaria brassicicola is an important, necrotrophic fungal pathogen that causes black spot disease on Brassicas. In order to study pathogenicity mechanisms, gene deletion mutants were generated for 21 putative regulatory genes including kinases and transcription factors subjectively selected from the annotated A. genome. Except Ste12, of SNF1 kinase, XlnR, CreA homologues control cell wall‐degrading enzyme production did not significantly affect virulence in contrast other...
Abstract Background Fungal plant pathogens cause serious agricultural losses worldwide. Alternaria arborescens is a major pathogen of tomato, with its virulence determined by the presence conditionally dispensable chromosome (CDC) carrying host-specific toxin genes. Genes encoding these toxins are well-studied, however genomic content and organization CDC not known. Results To gain richer understanding molecular determinants evolution pathogenicity, we performed whole genome sequencing A. ....
The fungal genus Alternaria is comprised of many saprophytic and endophytic species, but most well known as containing notoriously destructive plant pathogens. There are over 4,000 Alternaria/host associations recorded in the USDA Fungal Host Index ranking 10th among nearly 2,000 genera based on total number host records. While few species appear to have a sexual stage their life cycles, majority lack sexuality altogether. Many pathogenic prolific toxin producers, which facilitates...
The formation of appressoria, specialized plant penetration structures Magnaporthe oryzae, is regulated by the MST11-MST7-PMK1 MAP kinase cascade. One its downstream transcription factor, MST12, important for and invasive growth but dispensable appressorium formation. To identify additional targets that are Pmk1, in this study we performed phosphorylation assays with a protein microarray composed 573 M. oryzae factor (TF) genes. Three TF genes phosphorylated Pmk1 vitro were further analyzed...
Abstract Background The dimorphic fungus Histoplasma capsulatum causes respiratory and systemic disease in mammalian hosts by expression of factors that enable survival within phagocytic cells the immune system. ’s dimorphism is distinguished growth either as avirulent mycelia or pathogenic yeast. Geographically distinct strains differ their relative virulence production requirement for specific factors. close similarity genome sequences these diverse suggests phenotypic variations result...
Six unique expressed sequence tag (EST) libraries were generated from four developmental stages of Phytophthora sojae P6497. RNA was extracted mycelia, swimming zoospores, germinating cysts, and soybean (Glycine max (L.) Merr.) cv. Harosoy tissues heavily infected with P. sojae. Three created mycelia growing on defined medium, complex nutrient-limited medium. The 26,943 high-quality sequences obtained clustered into 7,863 unigenes composed 2,845 contigs 5,018 singletons. total number...
Significant progress has been made in defining the central signaling networks many organisms, but collectively we know little about downstream targets of these and genes they regulate. To reconstruct regulatory circuit calcineurin signal transduction via MoCRZ1, a Magnaporthe oryzae C2H2 transcription factor activated by dephosphorylation, used combined approach chromatin immunoprecipitation - chip (ChIP-chip), coupled with microarray expression studies. One hundred forty were identified as...
A fungal mycelium is typically composed of radially extending hyphal filaments interconnected by bridges created through anastomoses. These facilitate the dissemination nutrients, water, and signaling molecules throughout colony. In this study, we used targeted gene deletion nitrate utilization mutants cruciferous pathogen Alternaria brassicicola two closely related species to investigate fusion (anastomosis) its role in ability fungi cause disease. All eight A. isolates tested, as well...
Abstract Background Rice blast caused by the fungus Magnaporthe oryzae is an important disease in virtually every rice growing region of world, which leads to significant annual decreases grain quality and yield. To prevent disease, resistance genes have been cloned introduced into susceptible cultivars. However, can often be broken within few years release, due mutation cognate avirulence fungal field populations. Results better understand pattern M. isolates under natural selection forces,...
Aspergillus fumigatus is an opportunistic pathogen and allergen of mammals. Calcium signalling essential for A. pathogenicity regulated by the CrzA transcription factor. We used ChIP-seq (Chromatin Immunoprecipitation DNA sequencing) to explore gene targets in fumigatus. In total, 165 potential binding peaks including 102 directly genes were identified, resulting prediction A[GT][CG]CA[AC][AG] CrzA-binding motif. The putatively exhibited a diverse array functions. phkB (Afu3g12530) histidine...