A5055873317- Bioinformatics and Genomic Networks
- Genetic Associations and Epidemiology
- Fungal and yeast genetics research
- Glycosylation and Glycoproteins Research
- Health, Environment, Cognitive Aging
- Hemoglobinopathies and Related Disorders
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- RNA and protein synthesis mechanisms
- Genomics and Rare Diseases
- Biomedical Text Mining and Ontologies
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Iron Metabolism and Disorders
- Blood groups and transfusion
- Carbohydrate Chemistry and Synthesis
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Air Quality and Health Impacts
- Gene expression and cancer classification
- Lysosomal Storage Disorders Research
RTI International
2007-2025
North Carolina State University
2003-2021
Nantong University
2019
Taizhou People's Hospital
2019
Research Triangle Park Foundation
2008-2015
Queens University of Charlotte
2013
National Institute of Diabetes and Digestive and Kidney Diseases
2013
The Graduate Center, CUNY
2013
National Institutes of Health
2011
University of Oklahoma
1994-2001
Magnaporthe grisea is the most destructive pathogen of rice worldwide and principal model organism for elucidating molecular basis fungal disease plants. Here, we report draft sequence M. genome. Analysis gene set provides an insight into adaptations required by a fungus to cause disease. The genome encodes large diverse secreted proteins, including those defined unusual carbohydrate-binding domains. This also possesses expanded family G-protein-coupled receptors, several new...
The potential for genome-wide association studies to relate phenotypes specific genetic variation is greatly increased when data can be combined or compared across multiple studies. To facilitate replication and validation studies, RTI International (Research Triangle Park, North Carolina) the National Human Genome Research Institute (Bethesda, Maryland) are collaborating on consensus measures Phenotypes eXposures (PhenX) project. goal of PhenX identify 15 high-priority, well-established,...
Abstract Background The G-protein-coupled receptors (GPCRs) are one of the largest protein families in human and other animal genomes, but no more than 10 GPCRs have been characterized fungi. Do fungi contain only this handful or there to be discovered? We asked question using recently sequenced genome fungal plant pathogen Magnaporthe grisea . Results Proteins with significant similarity fungus-specific eukaryotic were identified M. These included homologs known GPCRs, cAMP from...
Abstract Background Rice blast disease is caused by the filamentous Ascomycetous fungus Magnaporthe oryzae and results in significant annual rice yield losses worldwide. Infection this many other fungal plant pathogens requires development of a specialized infection cell called an appressorium. The molecular processes regulating appressorium formation are incompletely understood. Results We analyzed genome-wide gene expression changes during spore germination on hydrophobic surface compared...
Lysosomal enzymes are targeted to the lysosome through binding mannose 6-phosphate receptors because their glycans modified with 6-phosphate. This modification is catalyzed by UDP-N-acetylglucosamine:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-phosphotransferase). Bovine GlcNAc-phosphotransferase was isolated using monoclonal antibody affinity chromatography, and an alpha2beta2gamma2-subunit structure proposed. Although cDNA encoding gamma-subunit has been described,...
Abstract Background Although the mouse is widely used to model human lung development, function, and disease, our understanding of molecular mechanisms involved in alveolarization peripheral incomplete. Recently, Molecular Atlas Lung Development Program (LungMAP) was funded by National Heart, Lung, Blood Institute develop an integrated open access database (known as BREATH) characterize cellular anatomy developing lung. To support this effort, we designed detailed anatomic ontologies...
We have sequenced a 1.1-Mb region of human chromosome 22q containing the dosage-sensitive gene(s) responsible for cat eye syndrome (CES) as well 450-kb homologous on mouse 6. Fourteen putative genes were identified within or adjacent to CES critical (CESCR), including three known ( IL-17R , ATP6E and BID ) nine novel genes, based EST identity. Two CECR3 CECR9 identified, in absence hits, by comparing segments genomic sequence around two solitary amplified exons, thus showing utility...
Over 28,000 expressed sequence tags (ESTs) were produced from cDNA libraries representing a variety of growth conditions and cell types. Several Magnaporthe grisea strains used to produce the libraries, including nonpathogenic strain bearing mutation in PMK1 mitogen-activated protein kinase. Approximately 23,000 ESTs could be clustered into 3,050 contigs, leaving 5,127 singleton sequences. The estimate 8,177 unique sequences indicates that over half genes fungus are represented ESTs....
The PhenX (consensus measures for Phenotypes and eXposures) Toolkit (https://www.phenxtoolkit.org/) offers high-quality, well-established of phenotypes exposures use by the scientific community. contains 295 drawn from 21 research domains (fields research). were selected Working Groups domain experts using a consensus process that included input provides description each measure, rationale including it in Toolkit, protocol(s) collecting supporting documentation. Users can browse measures,...
Abstract Background To explore the role of non‐coding RNA activated by DNA damage ( NORAD ), a long ribonucleic acid (lncRNA), in non‐small cell lung cancer (NSCLC) and its possible mechanism. Methods Quantitative real‐time polymerase chain reaction was adopted for detection expression levels , micro (miR)‐656‐3p, AKT serine/threonine kinase 1 AKT1 ). The effects miR‐656‐3p, on proliferation migration were examined through Cell Counting Kit‐8 (CCK‐8) Transwell assay. Subsequently, binding...
Oral mucositis is a common, dose-limiting, acute toxicity of radiation therapy administered for the treatment cancers head and neck. Accumulating data would suggest that pathogenesis complex involves sequential interaction all cell types oral mucosa, as well number cytokines elements environment. While studies have reported on gene expression particular in response to radiation, overall irradiated mucosa has only been evaluated limited way. The present study was undertaken evaluate target...
The National Institute of Diabetes and Digestive Kidney Diseases (NIDDK) Central Repository makes data biospecimens from NIDDK-funded research available to the broader scientific community. It thereby facilitates: testing new hypotheses without or biospecimen collection; pooling across several studies increase statistical power; informative genetic analyses using Repository's well-curated phenotypic data. This article describes initial database plan for its revision a simpler model. Among...
The goals of PhenX (consensus measures for Phenotypes and eXposures) are to promote the use standard measurement protocols help investigators identify opportunities collaborative research cross-study analysis, thus increasing impact individual studies. Toolkit (https://www.phenxtoolkit.org/) offers high-quality, well-established assess phenotypes exposures in studies with human participants. contains representing 29 domains 6 specialty collections that add depth specific areas (e.g.,...
Objectives Sickle cell disease (SCD) is a rare group of inherited red blood disorders that affect hemoglobin, resulting in serious multi-system complications. The limited number patients available to participate research studies can inhibit investigating sophisticated relationships. Secondary analysis method involves using existing data answer new questions. Data harmonization enables secondary by combining across studies, especially helpful for where individual may be small. National Heart,...
Population-based variability in protein expression patterns, especially humans, is often observed but poorly understood. Moreover, very little known about how interindividual genetic variation contributes to patterns. To begin address this, we describe elements of technical and biological variations contributing 544 proteins a population 24 individual human lymphoblastoid cell lines that have been extensively genotyped as part the International HapMap Project. We determined levels 10% were...
The need for collaborative and transparent sharing of COVID-19 clinical trial large-scale observational study data to accelerate scientific discovery inform practice is critical. Responsible data-sharing requires addressing challenges associated with privacy confidentiality, linkage, quality, variable harmonization, formats, comprehensive metadata documentation produce a high-quality, contextually rich, findable, accessible, interoperable, reusable (FAIR) dataset. This communication explores...
The PhenX Toolkit provides researchers with recommended, well-established, low-burden measures suitable for human subject research. database of Genotypes and Phenotypes (dbGaP) is the data repository a variety studies funded by National Institutes Health, including genome-wide association studies. dbGaP requires that investigators provide dictionary study variables as part submission process. Thus, unique resource can help identify share same or similar variables. As proof concept, from 16...