Jonathan A. Butler
A5072739848- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Nanoparticles: synthesis and applications
- Genomics and Chromatin Dynamics
- Bacterial biofilms and quorum sensing
- Antimicrobial agents and applications
- Graphene and Nanomaterials Applications
- Fungal and yeast genetics research
- Bacteriophages and microbial interactions
- Wound Healing and Treatments
- Metal complexes synthesis and properties
- RNA and protein synthesis mechanisms
- Genomic variations and chromosomal abnormalities
- Healthcare and Environmental Waste Management
- Glycosylation and Glycoproteins Research
- Carbon and Quantum Dots Applications
- Salmonella and Campylobacter epidemiology
- Animal Genetics and Reproduction
- Hypothalamic control of reproductive hormones
- X-ray Diffraction in Crystallography
- Plant-Microbe Interactions and Immunity
- Estrogen and related hormone effects
- Crystallization and Solubility Studies
- Phytochemistry and biological activities of Ficus species
- Historical Medical Research and Treatments
Manchester Metropolitan University
2017-2024
Westchester Medical Center
2022
New York Medical College
2022
American College of Surgeons
2022
Interface (United Kingdom)
2020
The University of Texas at Austin
2019
University of Sheffield
2017-2018
University of Manchester
2010-2015
King's College London
2001-2010
Broad Institute
2004-2008
Neurospora crassa is a central organism in the history of twentieth-century genetics, biochemistry and molecular biology. Here, we report high-quality draft sequence N. genome. The approximately 40-megabase genome encodes about 10,000 protein-coding genes--more than twice as many fission yeast Schizosaccharomyces pombe only 25% fewer fruitfly Drosophila melanogaster. Analysis gene set yields insights into unexpected aspects biology including identification genes potentially associated with...
Magnaporthe grisea is the most destructive pathogen of rice worldwide and principal model organism for elucidating molecular basis fungal disease plants. Here, we report draft sequence M. genome. Analysis gene set provides an insight into adaptations required by a fungus to cause disease. The genome encodes large diverse secreted proteins, including those defined unusual carbohydrate-binding domains. This also possesses expanded family G-protein-coupled receptors, several new...
Ustilago maydis is an important fungal pathogen of maize, causing corn smut. It well adapted to its host and proliferates in living plant tissue without inducing a defence response. The genome sequence U. has now been determined, the first for biotrophic parasite. Several gene clusters that encode secreted proteins unknown function were identified: genome-wide expression analysis shows clustered genes are upregulated during disease. Mutations these frequently affect virulence, ranging from...
New DNA sequencing technologies deliver data at dramatically lower costs but demand new analytical methods to take full advantage of the very short reads that they produce. We provide an initial, theoretical solution challenge de novo assembly from whole-genome shotgun "microreads." For 11 genomes sizes up 39 Mb, we generated high-quality assemblies 80x coverage by paired 30-base simulated modeled after real Illumina-Solexa reads. The bacterial Campylobacter jejuni and Escherichia coli...
We previously described the whole-genome assembly program Arachne, presenting assemblies of simulated data for small to mid-sized genomes. Here we describe algorithmic adaptations program, allowing mammalian-size genomes, and also improving smaller Three principal changes were simultaneously made applied mouse genome, during a six-month period development: (1) Supercontigs (scaffolds) iteratively broken rejoined using several criteria, yielding 64-fold increase in length (N50), apparent...
Although often considered “minimal” organisms, mycoplasmas show a wide range of diversity with respect to host environment, phenotypic traits, and pathogenicity. Here we report the complete genomic sequence proteogenomic map for piscine mycoplasma Mycoplasma mobile , noted its robust gliding motility. For first time, proteomic data are used in primary annotation new genome, providing validation expression many predicted proteins. Several novel features were discovered including long...
The effects of metal ion solutions (silver, copper, platinum, gold and palladium) were determined individually in combination against Enterococcus faecium, Acinetobacter baumannii Klebsiella pneumoniae. Platinum, palladium showed the greatest antimicrobial efficacy zone inhibition (ZoI) assays. When tested combinations using ZoI assays, gold/platinum, gold/palladium platinum/palladium indicative synergy. Microbial inhibitory concentration demonstrated platinum pneumoniae silver optimal....
Abstract Double‐label immunohistochemistry was employed to establish whether immunoreactivity for the β subtype of oestrogen receptor (ERβ‐IR) is present in gonadotropin releasing hormone (GnRH)‐containing cells. In immortalized GnRH cell line, GT1‐7, almost all nuclei were immunoreactive ERβ. preoptic area ovariectomized rats, more than one‐half neurones (52.0–63.5%) contained ERβ‐IR within nucleus; a smaller proportion these (5–10%) displayed particularly intense nuclear signal The...
ABSTRACT The Gram-negative bacterium Campylobacter jejuni encodes an extensively characterized N-linked protein glycosylation system that modifies many surface proteins with a heptasaccharide glycan. In C. , the genes encode enzymes required for glycan biosynthesis and transfer to are located at single pgl gene locus. Similar loci also present in genome sequences of all other species, although variations content organization evident. this study, we have demonstrated only species closely...
Fatty alcohols are important industrial oleochemicals with broad applications and a growing market. Here, we sought to engineer Yarrowia lipolytica serve as renewable source of fatty (specifically hexadecanol, heptadecanol, octadecanol, oleyl alcohol) directly from glucose. Through screening four acyl-CoA reductase (FAR) enzyme variants across two engineered background strains, identified that MhFAR enabled the highest production. Further strain engineering, fed-batch flask cultivation,...
The development of surfaces as antimicrobial materials is important to the food industry. This study investigated potential a range metal coated including silver, titanium, copper, iron, molybdenum, zinc and silicon (control) against Staphylococcus aureus, Escherichia coli Listeria monocytogenes. leaching metals were measured by inductively coupled plasma-atomic adsorption spectroscopy compared antibacterial activity using nitroblue tetrazolium assay an adapted BS ISO 22196:2011 standard....
Alginate is a linear biodegradable polysaccharide polymer, which bio-renewable and widely used for various biomedical applications. For the next generation of medical textiles, alginate nanofibres are desirable their use in wound dressings that biocompatible, sustainable, abundantly available. This study has developed unique manufacturing process producing with exceptional antimicrobial properties oregano essential oil (OEO) as natural agent. OEO varying degrees concentration was...
The Campylobacter jejuni flagellin protein is O- glycosylated with structural analogues of the nine-carbon sugar pseudaminic acid. most common modifications in C. 81-176 strain are 5,7-di- N- acetylated derivative (Pse5Ac7Ac) and an acetamidino-substituted version (Pse5Am7Ac). Other structures detected include acetylglutamine-substituted derivatives (Pse5Am7Ac8OAc Pse5Am7Ac8GlnNAc, respectively). Recently, a acid modified di- methylglyceroyl group was NCTC 11168 strain. gene products...
Carbon monoxide-releasing molecules (CORMs) are a promising class of new antimicrobials, with multiple modes action that distinct from those standard antibiotics. The relentless increase in antimicrobial resistance, exacerbated by lack antibiotics, necessitates better understanding how such novel agents act and might be used synergistically established This work aimed to understand the mechanism(s) underlying synergy between manganese-based photoactivated carbon molecule (PhotoCORM), [Mn(CO)...