A5017537645- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Chromosomal and Genetic Variations
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Growth Hormone and Insulin-like Growth Factors
- Mycotoxins in Agriculture and Food
- Genetics and Neurodevelopmental Disorders
- Thyroid Disorders and Treatments
- Wheat and Barley Genetics and Pathology
- Bone Metabolism and Diseases
- Peroxisome Proliferator-Activated Receptors
- Transgenic Plants and Applications
- Plant Gene Expression Analysis
- Bone health and treatments
- Insect Resistance and Genetics
- Metabolism and Genetic Disorders
- Genomic variations and chromosomal abnormalities
The Ohio State University
2011-2024
Ospedale Santa Chiara
2011-2022
North Carolina State University
2007-2020
Chinese Academy of Agricultural Sciences
2017
Institute of Plant Protection
2017
Sokoine University of Agriculture
2016-2017
University of Arkansas at Fayetteville
2016-2017
International Rice Research Institute
2017
University of Exeter
2016-2017
International Livestock Research Institute
2017
Abstract The broad-spectrum rice blast resistance gene Pi9 was cloned using a map-based cloning strategy. Sequencing of 76-kb bacterial artificial chromosome (BAC) contig spanning the locus led to identification six tandemly arranged resistance-like genes with nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) (Nbs1-Pi9–Nbs6-Pi9). Analysis selected deletion mutants transformation 45-kb fragment from BAC into susceptible cultivar TP309 narrowed down candidate Nbs2-Pi9 Nbs3-Pi9....
Although the functions of a few effector proteins produced by bacterial and oomycete plant pathogens have been elucidated in recent years, information for vast majority pathogen effectors is still lacking, particularly those plant-pathogenic fungi. Here, we show that avirulence AvrPiz-t from rice blast fungus Magnaporthe oryzae preferentially accumulates specialized structure called biotrophic interfacial complex then translocated into (Oryza sativa) cells. Ectopic expression transgenic...
The rice blast resistance (R) genes Pi2 and Piz-t confer broad-spectrum against different sets of Magnaporthe grisea isolates. We first identified the gene using a map-based cloning strategy. is member cluster comprising nine members (named Nbs1-Pi2 to Nbs9-Pi2) encodes protein with nucleotide-binding site leucine-rich repeat (LRR) domain. Fine genetic mapping, molecular characterization susceptible mutants, complementation tests indicated that Nbs4-Pi2 gene. gene, allele in cultivar Toride...
Most transgenic crops are produced through tissue culture. The impact of utilizing such methods on the plant epigenome is poorly understood. Here we generated whole-genome, single-nucleotide resolution maps DNA methylation in several regenerated rice lines. We found that all tested plants had significant losses compared to non-regenerated plants. Loss was largely stable across generations, and certain sites genome were particularly susceptible loss methylation. at promoters associated with...
Abstract The Arabidopsis thaliana resistance gene RPW8 triggers the hypersensitive response (HR) to restrict powdery mildew infection via salicylic acid–dependent signaling pathway. To further understand how is regulated, we have conducted a genetic screen identify mutations enhancing RPW8-mediated HR-like cell death (designated erh). Here, report isolation and characterization of erh1 mutant, in which At2g37940 locus knocked out by T-DNA insertion. Loss function ERH1 results acid...
Abstract Histone acetylation and deacetylation play an important role in the modification of chromatin structure regulation gene expression eukaryotes. Chromatin status is modulated antagonistically by histone acetyltransferases deacetylases (HDACs). In this study, we characterized function deacetylase701 (HDT701), a member plant-specific HD2 subfamily HDACs, rice (Oryza sativa) innate immunity. Transcription HDT701 increased compatible reaction decreased incompatible after infection fungal...
Although nucleotide-binding domain, leucine-rich repeat (NLR) proteins are the major immune receptors in plants, mechanism that controls their activation and signaling remains elusive. Here, we report avirulence effector AvrPiz-t from Magnaporthe oryzae targets rice E3 ligase APIP10 for degradation, but APIP10, return, ubiquitinates thereby causes its degradation. Silencing of non-Piz-t background compromises basal defense against M. oryzae. Conversely, silencing Piz-t cell death,...
Interactions between rice and Magnaporthe oryzae involve the recognition of cellular components exchange complex molecular signals from both partners. How these interactions occur in cells is still elusive. We employed robust-long serial analysis gene expression, massively parallel signature sequencing, sequencing by synthesis to examine transcriptome profiles infected leaves. A total 6,413 planta–expressed fungal genes, including 851 genes encoding predicted effector proteins, were...
The ubiquitin proteasome system in plants plays important roles plant-microbe interactions and immune responses to pathogens. We previously demonstrated that the rice U-box E3 ligase SPL11 its Arabidopsis ortholog PUB13 negatively regulate programmed cell death (PCD) defense response. However, components involved SPL11/PUB13-mediated PCD signaling pathway remain unknown. In this study, we report SPL11-interacting Protein 6 (SPIN6) is a Rho GTPase-activating protein (RhoGAP) interacts with...
Nucleotide-binding leucine-rich repeat (NLR) proteins play critical roles in plant immunity. However, how NLRs are regulated and activate defense signaling is not fully understood. The rice (Oryza sativa) NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10 (APIP10) negatively regulates accumulation. In this study, we found that APIP10 interacts with two transcription factors, VASCULAR PLANT...
DNA 5-methylcytosine (5-meC) is an important epigenetic mark for transcriptional gene silencing in many eukaryotes. In Arabidopsis, 5-meC glycosylase/lyases actively remove to counteract a locus-specific manner, and have been suggested maintain the expression of transposons. However, it unclear whether plant demethylases can promote transposition Here we report functional characterization glycosylase/lyase DNG701 rice. encodes large (1,812 amino acid residues) glycosylase domain protein....
Summary Bowman–Birk trypsin inhibitors (BBIs) play important roles in animal and plant immunity, but how these protease are involved the immune system remains unclear. Here, we show that rice ( Oryza sativa ) BBI protein APIP4 is a common target of fungal effector an NLR receptor for innate immunity. exhibited inhibitor activity vitro vivo . Knockout enhanced susceptibility, overexpression increased resistance to pathogen Magnaporthe oryzae The M. AvrPiz‐t interacted with suppressed...
Wheat blast, caused by the fungus Magnaporthe oryzae Triticum (MoT) pathotype, is a devastating disease persistent in South America and Bangladesh. Since MoT generally fails to cause visual symptoms wheat until heading stage when infection would have advanced, control fungicide application solely based on detection of ineffective. To develop an accurate sensitive method detect at seedling vegetative stages for control, we sequenced genomes two isolates from Brazil identified DNA fragments,...
Twenty-five medical centers and the Prader-Willi Syndrome (PWS) Association collaborated on a study which attempted to identify all people with genetically confirmed diagnosis of PWS living in Italy. Investigators participating contacted subjects and/or their family, filled specially developed form required data forwarded this information by email. The identified 425 (209 males 216 females, between ages 0.4-46.7). Two hundred thirty-eight patients had del15, 104 UPD15, 4 demonstrated...
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants. RNA-guided nuclease can be used to not only generate precise genomic mutations, but also manipulate gene expression when present as deactivated protein (dCas9).In this study, we describe vector toolkit analyzing dCas9-mediated activation (CRISPRa) or inactivation (CRISPRi) of maize protoplasts. An improved protoplast isolation and transfection...
Rice blast disease is emerging as a major constraint to rice production in Africa. Although traditional gene-tagging strategy using biparental crosses can effectively identify resistance (R) genes or quantitative trait loci (QTL) against Magnaporthe oryzae, the mapping procedure required time consuming and requires many populations investigate genetics of resistance. In this report, we conducted genome-wide association study (GWAS) rapidly map conferring eight M. oryzae isolates from four...
Background; Alström syndrome (AS) is characterized by core clinical features, including cone-rod dystrophy, early-onset obesity, progressive bilateral sensorineural hearing loss, type 2 diabetes mellitus, and cardiomyopathy. Age-specific diagnostic criteria have been proposed. Early diagnosis critical due to the variable nature of manifestations. The limited number reported cases hinders a comprehensive understanding natural history disease. Methods; We present three pediatric AS,...
despite recent progress in imaging, it is still difficult to distinguish between pituitary adenoma and hyperplasia, even using Magnetic Resonance Imaging (MRI) with gadolinium injection. We describe an example of reactive hyperplasia from primary hypothyroidism that mimicked a macroadenoma child. 10 year old boy presented headache statural growth arrest. MRI revealed intrasellar suprasellar mass. Endocrine evaluation hypothyroidism. the patient was started on levothyroxine resolution mass...
Rice blast disease, caused by the fungal pathogen Magnaporthe grisea, is an excellent model system to study plant-fungal interactions and host defense responses. In this study, comprehensive analysis of rice (Oryza sativa) transcriptome after M. grisea infection was conducted using robust-long serial gene expression. A total 83,382 distinct 21-bp expression tags were identified from 627,262 individual isolated resistant (R), susceptible (S), control (C) libraries. Sequence revealed that in R...
Summary Magnaporthe oryzae is an important fungal pathogen of both rice and wheat. However, how M. effectors modulate plant immunity not fully understood. Previous studies have shown that the effector AvrPiz‐t targets host ubiquitin‐proteasome system to manipulate defence. In return, two ubiquitin E3 ligases, APIP6 APIP10, ubiquitinate for degradation. To determine lysine residues contribute stability function AvrPiz‐t, we generated double (K1,2R‐AvrPiz‐t), triple (K1,2,3R‐AvrPiz‐t) l ysine‐...