A5053197323- Insect-Plant Interactions and Control
- Research on scale insects
- Plant and animal studies
- Hymenoptera taxonomy and phylogeny
- Insect behavior and control techniques
- Nematode management and characterization studies
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Genetic diversity and population structure
- Insect Pest Control Strategies
- Insect and Arachnid Ecology and Behavior
- Biological Control of Invasive Species
- Genomics and Phylogenetic Studies
- Hemiptera Insect Studies
- Agriculture and Rural Development Research
- Entomopathogenic Microorganisms in Pest Control
- Agricultural pest management studies
- Nuclear Physics and Applications
- Animal Ecology and Behavior Studies
- Plant Parasitism and Resistance
- Forest Insect Ecology and Management
- Legume Nitrogen Fixing Symbiosis
- Polar Research and Ecology
- Ecology and Vegetation Dynamics Studies
- Insect symbiosis and bacterial influences
Institut Sophia Agrobiotech
2015-2024
Centre National de la Recherche Scientifique
2013-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2024
Université Côte d'Azur
2010-2024
Institut de Biologie Valrose
2024
Observatoire de la Côte d’Azur
2018
Institut National de la Recherche Agronomique
2005-2014
Institut Agro Montpellier
2009-2011
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2006-2011
Institut de Recherche pour le Développement
2006-2011
Abstract Summary: QDD is an open access program providing a user-friendly tool for microsatellite detection and primer design from large sets of DNA sequences. The designed to deal with all steps treatment raw sequences obtained pyrosequencing enriched libraries, but it also applicable data through other sequencing methods, using FASTA files as input. following tasks are completed by QDD: tag sorting, adapter/vector removal, elimination redundant sequences, possible genomic multicopies...
Recent studies of the routes worldwide introductions alien organisms suggest that many widespread invasions could have stemmed not from native range, but a particularly successful invasive population, which serves as source colonists for remote new territories. We call here this phenomenon bridgehead effect. Evaluating likelihood such scenario is heuristically challenging. solved problem by using approximate Bayesian computation methods to quantitatively compare complex invasion scenarios...
Abstract Background The rapid evolution of 454 GS-FLX sequencing technology has not been accompanied by a reassessment the quality and accuracy sequences obtained. Current strategies for decision-making error-correction are based on an initial analysis Huse et al. in 2007, older GS20 system experimental sequences. We analyze here data identify factors playing role error, through use extensive dataset Roche control DNA fragments. Results obtained mean error rate 1.07%. More importantly, is...
Abstract Microsatellites (or SSRs: simple sequence repeats) are among the most frequently used DNA markers in many areas of research. The use microsatellite is limited by difficulties involved their de novo isolation from species for which no genomic resources available. We describe here a high‐throughput method isolating based on coupling multiplex enrichment and next‐generation sequencing 454 GS‐FLX Titanium platforms. procedure was calibrated model ( Apis mellifera ) validated 13 other...
Abstract Reducing pesticide use has become a goal shared by several European countries and major issue in public policies due to the negative impacts of pesticides on environment human health. However, since most agri-food sector relies these countries, substantially reducing is complex issue. To overcome this situation, we argue that agricultural research role play must adopt pesticide-free paradigm expect deep impact use. In article, explain why new needed outline fronts it will help...
Abstract Populations are introduced into novel environments in different contexts, one being the biological control of pests. Despite intense efforts, less than half agents establish. Among possible approaches to improve control, is better understand processes that underpin introductions and contribute ecological evolutionary success. In this perspective, we first review demographic genetic at play small populations, be they stochastic or deterministic. We discuss theoretical outcomes these...
Inferences about introduction histories of invasive species remain challenging because the stochastic demographic processes involved. Approximate Bayesian computation (ABC) can help to overcome these problems, but such method requires a prior understanding population structure over study area, necessitating use alternative methods and an intense sampling design. In this study, we made inferences worldwide invasion history ladybird Harmonia axyridis by various genetics statistical methods,...
Although a growing body of work supports the plausibility sympatric speciation in animals, practical difficulties directly quantifying reproductive isolation between diverging taxa remain an obstacle to analyzing this process. We used combination genetic and biogeochemical markers produce direct field estimate assortative mating phytophagous insect populations. show that individuals same species, European corn borer Ostrinia nubilalis, develop on different host plants can display almost...
Abstract Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range crops and ornamental plants worldwide. Their high degree morphological similarity makes them difficult to identify limits their study management. We aimed set markers for the genetic characterization identification complexes taxa in Pseudococcidae. surveyed tested used previous studies then identified new particularly relevant genomic regions which no satisfactory were available. all on subset four distributed...
EU agriculture is currently in transition from conventional crop protection to integrated pest management (IPM). Because biocontrol a key component of IPM, many European countries recently have intensified their national efforts on research and innovation (R&I), although such initiatives are often fragmented. The operational outputs would benefit closer collaboration among stakeholders via transnationally coordinated approaches, as most economically important pests similar across Europe....
Abstract BACKGROUND: The spider mite, Tetranychus urticae , is the major strawberry pest in Brazil. main strategies for its control comprise synthetic acaricides and predatory mites. recent register of a commercial formula azadirachtin (Azamax ® 12 g L −1 ) can be viable T. . In this work, effects on compatibility with mites Neoseiulus californicus Phytoseiulus macropilis crop were evaluated. RESULTS: Azadirachtin was efficient against mortality rate similar to that abamectin. addition,...
Abstract Most models of ecological speciation concern phytophagous insects in which is thought to be driven by host shifts and subsequent adaptations populations. Despite the ever‐increasing number studies, current evolutionary status most remains incompletely resolved, as estimates gene flow between taxa remain extremely rare. We studied population genetics two Ostrinia genus — one feeding mainly on maize other mugwort hop occurring sympatry throughout France. The actual level divergence...
Abstract This article documents the addition of 205 microsatellite marker loci to Molecular Ecology Resources Database. Loci were developed for following species: Bagassa guianensis, Bulweria bulwerii, Camelus bactrianus, Chaenogobius annularis, Creontiades dilutus, Diachasmimorpha tryoni, Dioscorea alata, Euhrychiopsis lecontei, Gmelina arborea, Haliotis discus hannai, Hirtella physophora, Melanaphis sacchari, Munida isos, Thaumastocoris peregrinus and Tuberolachnus salignus . These...
Microsatellites are the most popular source of molecular markers for studying population genetic variation in eukaryotes. However, few data currently available about their genomic distribution and abundance across phylum Nematoda. The recent completion genomes several nematode species, including Meloidogyne incognita, a major agricultural pest worldwide, now opens way comparative survey analysis microsatellites these organisms. Using MsatFinder, total numbers 1-6 bp perfect detected complete...
Abstract Pseudococcidae (mealybugs) is a large taxonomic group, including number of agronomic pests. Taxonomic identification mealybug species recurrent problem and represents major barrier to the establishment adequate pest management strategies. We combined molecular analysis three DNA markers (28S-D2, cytochrome oxidase I internal transcribed spacer 2) with morphological examination, for 176 specimens collected from 40 populations infesting various crops ornamental plants in Egypt France....
Range expansions are key processes shaping the distribution of species; their ecological and evolutionary dynamics have become especially relevant today, as human influence reshapes ecosystems worldwide. Many attempts to explain predict range assume, explicitly or implicitly, so‐called ‘pulled' expansion dynamics, in which low‐density edge populations provide most ‘fuel' for species advance. Some expansions, however, exhibit very different with high‐density behind front ‘pushing' forward....
Mealybugs (Hemiptera: Pseudococcidae) are pests constraining the international trade of Brazilian table grapes. They damage grapes by transmitting viruses and toxins, causing defoliation, chlorosis, vigor losses favoring development sooty mold. Difficulties in mealybug identification remain an obstacle to adequate management these pests. In this study, our primary aim was identify principal species infesting major grape-producing regions Brazil, morphological molecular characterization. Our...
Delottococcus aberiae De Lotto (Hemiptera: Pseudococcidae) is a mealybug of Southern African origin that has recently been introduced into Eastern Spain. It causes severe distortions on young citrus fruits and represents growing threat to Mediterranean production. So far, biological control proven unsatisfactory due the absence efficient natural enemies in Hence, management this pest currently relies only chemical control. The introduction D. from native area sustainable economically viable...
Parasitoid wasps rely primarily on venom to suppress the immune response and regulate physiology of their host. Intraspecific variability protein composition has been documented in some species, but its evolutionary potential is poorly understood. We performed an experimental evolution initiated with crosses two lines Leptopilinaboulardi different generate create new combinations factors. The offspring were maintained for 10 generations strains Drosophila melanogaster differing...
Abstract Target pests may become resistant to Bacillus thuringiensis (Bt) toxins produced by trangenic maize ( Zea mays L.). Untreated refuge areas are set aside conserve high frequencies of susceptibility alleles: a delay in resistance evolution is expected if susceptible individuals from refuges mate randomly with Bt fields. In principle, can be toxin‐free or any other plant, provided it hosts sufficiently large pest populations mating Bt‐maize Our aim was examine the suitability several...
Abstract French populations of the European corn borer consist two sympatric and genetically differentiated host races. As such, they are well suited to study processes that could be involved in speciation, but initial conditions host-race divergence need elucidated. Gene genealogies can provide insight into speciation. We used DNA sequences four nuclear genes (1) document genetic structure races previously delineated with allozyme markers, (2) find directly or indirectly reproductive...
Currently it remains difficult to obtain robust microsatellite markers for Lepidoptera.In an attempt overcome the problems associated with developing this insect order we combined (i) biotin-enrichment protocol, (ii) next generation pyrosequencing (through 454 GS-FLX Titanium technology) and (iii) use of individuals collected from eight geographically distant European populations representing three subspecies Euphydryas aurinia.Out 96 stringently designed primer pairs, 12 polymorphic loci...
Mealybugs (Hemiptera: Pseudococcidae) are common invasive pests in Europe, causing major problems on crops and ornamental plants. However, very few data available concerning the mealybug fauna of southern Europe. This lack difficulty identifying mealybugs morphologically by traditional techniques currently limit perspectives for efficient specific pest management. The aim this study was to provide multi-criterion characterization surveyed eastern Spain order facilitate their routine...
Abstract Mealybugs are major pests of grapevines worldwide. They cause economic losses by lowering the cosmetic value fruits, reducing yields, transmitting viruses and resulting in quarantine or rejection produce international trade. Knowledge species present a vineyard is important for adjustment management strategies. We surveyed accurately characterized mealybugs infesting vineyards one main production areas Chile; 164 were sampled from 26 four regions Chile identified DNA sequencing two...