A5104534651- Herpesvirus Infections and Treatments
- HIV Research and Treatment
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Animal Disease Management and Epidemiology
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
- Vector-Borne Animal Diseases
- Mycobacterium research and diagnosis
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Tuberculosis Research and Epidemiology
- Viral Infections and Immunology Research
- Mosquito-borne diseases and control
- Microbial infections and disease research
- RNA Interference and Gene Delivery
- Blood groups and transfusion
- Virology and Viral Diseases
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Animal Virus Infections Studies
University of Edinburgh
2006-2025
Roslin Cells (United Kingdom)
2018-2025
University of Florida
2017
University of Bern
1995
Centre de Recherche en Neurobiologie - Neurophysiologie de Marseille
1994
Institut de Virologie
1994
Inserm
1994
Addenbrooke's Hospital
1981-1985
Lancaster University
1978
ABSTRACT Lentivirus infections in small ruminants represent an economic problem affecting several European countries with important sheep-breeding industries. Programs for control and eradication of these are being initiated require reliable screening assays. This communication describes the construction evaluation a new serological enzyme-linked immunosorbent assay (ELISA) detection antibodies to maedi-visna virus (MVV) sheep caprine arthritis encephalitis (CAEV) goats. The solid phase is...
Abstract Bovine tuberculosis is a contagious bacterial disease of worldwide economic, zoonotic and welfare importance caused mainly by Mycobacterium bovis infection. Current regulatory diagnostic methods lack sensitivity require improvement. We have developed multiplex serological test for bovine here we provide an estimate the accuracy in cattle. Positive negative reference serum samples were obtained from animals Europe United States America. The specificity was 98.4% 99.7% using high...
ABSTRACT A major route of transmission Visna/maedi virus (VMV), an ovine lentivirus, is thought to be via the respiratory tract, by inhalation either cell-free or cell-associated virus. In previous studies, we have shown that infection lower tract much more efficient than upper tissues (T. N. McNeilly, P. Tennant, L. Lujan, M. Perez, and G. D. Harkiss, J. Gen. Virol. 88 :670-679, 2007). Alveolar macrophages (AMs) are prime candidates for initial uptake in lung, given their vivo tropism VMV,...
The aim of this work was to investigate whether an enzyme-linked immunosorbent assay (ELISA) useful for early detection maedi-visna virus (MVV) infection in sheep under field conditions. An ELISA based on p25 recombinant protein and a gp46 synthetic peptide used. Sequentially obtained serum samples ( n = 1,941) were studied 4 years. results compared with those the agar gel immunodiffusion (AGID) test, both tests reference result established using consensus scores at least 2 3 serologic...
Abstract Two groups of five sheep (7 months age) were moved and isolated in pens which did not allow visual tactile contact with other for 24 h. They then back to their original where was possible. After h the procedure repeated seven times one group (group 8-1) thirteen 14-1). One (control) remained When lambs spent more time standing still an alert posture, less eating resting, vocalized than control lambs. The heart rate increased when they between during isolation. plasma concentration...
This study was undertaken to determine the seroprevalence of Q fever in domestic livestock Saudi Arabia. Serum samples from 489 camels, 428 cattle, 630 sheep and 423 goats, either sex, different localities were tested for antibodies against C. burnetii using a indirect enzyme-linked immunosorbent assay (ELISA). A subsample 307 animals species simultaneously by ELISA immunofluorescence (IFA). The overall 30.71%. Prevalence 51.53%, 30.67%, 34.04% 12.38% goats sheep, respectively. Significant...
Bovine tuberculosis is usually diagnosed using tuberculin skin tests or at post-mortem. Recently, we have developed a serological test for bovine in cattle which shows high degree of accuracy serum samples. Here, assessed the performance individual milk The diagnostic specificity estimate sensitivity setting was 99.7% (95% CI: 99.2–99.9). This not altered significantly by boosting. relative estimates samples from comparative positive animals 90.8% 87.1–93.6) with In lesions, 96.0%...
Abstract Dendritic cells isolated from sheep afferent lymph were examined for their ability to bind soluble protein and peptide antigens labeled with fluorescein both in vitro assays following intradermal injection of antigen vivo . Analysis dendritic by flow cytometry revealed weak direct binding proteins antigens. However, the degree uptake was greatly enhanced presence specific antibody or if injected intradermally into antigen‐primed sheep. About 60% possessed take up experiments. The...
Sera from patients with giant cell arteritis and/or polymyalgia rheumatica have been found to contain increased levels of circulating immune complexes (IC). Results the polyethyleneglycol precipitation complement consumption (PEG-CC) assay correlated disease activity. 44% samples an active untreated group (21 patients) had ICs compared 23% inactive treated (49 patients). Further analysis was performed by 125I-Clq binding, PEG-C4 test, and 125I-conglutinin binding assays. Although we did not...
The main routes of transmission Visna/maedi virus (VMV), an ovine lentivirus, are thought to be through ingestion infected colostrum and/or milk or inhalation respiratory secretions. Whereas oral appears mediated via epithelial cells within the small intestine, mechanism uptake in tract is unknown. In addition, it not known whether infection by cell-associated cell-free VMV, previous studies having addressed this question. Intratracheal (i.t.) injection VMV a highly efficient method...
Tuberculosis in goats is usually diagnosed clinically, at postmortem, or by a positive skin test. However, none of these approaches detects all infected animals. Serology offers an additional tool to identify animals missed current tests. We describe the use Enferplex Caprine TB serology test aid management large dairy goat herd undergoing tuberculosis breakdown. Initial and testing showed that IgG antibodies were present both serum milk from 100% test-positive 77.8 95.4% test-negative...
In small ruminant lentivirus infections, cellular immune responses are diminished in clinically affected animals. The underlying mechanisms for this unknown. study, we tested the hypothesis that alterations expression of co-stimulatory molecules B7-1 and B7-2 involved infections with Visna/Maedi virus (VMV), prototype sheep. We studied B7 levels ex vivo peripheral blood mononuclear cells (PBMCs), determining RNA by real time reverse transcriptase polymerase chain reaction asymptomatic as...