A5103014685- Muscle Physiology and Disorders
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- melanin and skin pigmentation
- Molecular Biology Techniques and Applications
- Glaucoma and retinal disorders
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Cancer-related Molecular Pathways
- Cytomegalovirus and herpesvirus research
- Cardiac Valve Diseases and Treatments
- Infective Endocarditis Diagnosis and Management
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Fungal Biology and Applications
- Skin Protection and Aging
- RNA regulation and disease
- Mycorrhizal Fungi and Plant Interactions
- CAR-T cell therapy research
- Olfactory and Sensory Function Studies
BioCruces Health research Institute
2016-2021
Biomedal (Spain)
2013
Biogipuzkoa Health Research Institute
2009
Agrobiotechnology Institute
2006
University of the Basque Country
2000-2006
Gobierno de Aragón
2005
Consejo Superior de Investigaciones Científicas
2005
Instituto de Investigaciones Avícolas
1992
Although the combination of pale skin and intense sun exposure results in an important health risk for individual, it is less clear if at population level this has possessed evolutionary meaning. In sense, a number adaptive hypotheses have been put forward to explain evolution human pigmentation, such as photoprotection against sun-induced cancer, sexual selection, vitamin D synthesis or photolabile compounds, among others. It expected that pigmentation adaptive, we might be able see...
Gene editing methods are an attractive therapeutic option for Duchenne muscular dystrophy, and they have immediate application in the generation of research models. To generate myoblast cultures that could be useful vitro drug screening, we optimised a CRISPR/Cas9 gene edition protocol. We successfully used it wild type immortalised myoblasts to delete exon 52 dystrophin gene, modelling common dystrophy mutation; patient's deleted inhibitory microRNA target region utrophin UTR, leading...
Duchenne muscular dystrophy is a lethal disease caused by lack of dystrophin. Skipping exons adjacent to out-of-frame deletions has proven restore dystrophin expression in patients. Exon 51 been the most studied target both preclinical and clinical settings availability standardized procedures quantify exon skipping would be advantageous for evaluation data.To compare methods currently used antisense oligonucleotide-induced DMD transcript provide guidance about method use.Six laboratories...
Aims New therapies for neuromuscular disorders are often mutation specific and require to be studied in patient's cell cultures. In Duchenne muscular dystrophy (DMD) dystrophin restoration drugs being developed but as muscle cultures from DMD patients scarce do not grow or differentiate well, only a limited number of candidate tested. Moreover, quantification by western blotting requires large cultured cells; so fewer compounds thoroughly screened is desirable. We aimed develop quantitative...
To evaluate the sensitivity and specificity of a PCR-strip system based on reverse dot blot for detection MUC5AC mRNA in corneal epithelium samples from patients with limbal stem cell deficiency (LSCD), to determine correlation clinical diagnosis.We obtained 87 impression cytology (IC) 55 subjects (37 clinically diagnosed LSCD 18 control subjects). Total RNA was extracted each IC sample retrotranscribed cDNA. transcript amplified by customized RT-PCR assay detected PCR strips hybridization...
Excessive subconjunctival scarring is the main reason of failure glaucoma filtration surgery. We analyzed conjunctival and systemic gene expression patterns after non penetrating deep sclerectomy (NPDS). To find related to surgical their correlation with clinical outcomes. This study consisted two consecutive stages. The first was a prospective analysis wound-healing profile six patients NPDS. Conjunctival samples peripheral blood were collected before 15, 90,180, 360 days In second stage,...
Copy Number Variants (CNVs) contribute to a large fraction of genetic diversity and some them have been reported offer an evolutionary advantage.To identify CNVs in pigmentary loci that could human skin pigmentation diversity.This study assessed the existence every exon candidate genes: TYR, TYRP1, DCT, MC1R SLC24A5, using Multiplex Amplifiable Probe Hybridization technique (MAPH). This analysed total 99 DNA samples unrelated individuals from different populations. Validation further...
Corrosion has a major impact on the economics of facilities, structures, and equipment in oil gas industry. Cathodic protection by sacrificial anodes is implemented to prevent, avoid, minimize corrosion damage. This article describes development, testing, application local anode Mexico made from an aluminum alloy.
Abstract Gene edition methods are an attractive putative therapeutic option for Duchenne muscular dystrophy and they have immediate application in the generation of research models. To generate two new edited myoblast cultures that could be useful vitro drug screening, we optimised a CRISPR/Cas9 gene protocol. We successfully used it wild type immortalised myoblasts to delete exon 52 dystrophin gene: DMDΔ52-Model, modelling common mutation; patient’s deleted inhibitory microRNA target region...