A5041343197- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Genetic and phenotypic traits in livestock
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Infectious Diseases and Mycology
- RNA modifications and cancer
- Genetic diversity and population structure
- Molecular Biology Techniques and Applications
- Animal Disease Management and Epidemiology
- Immune Response and Inflammation
- Mycobacterium research and diagnosis
- Virus-based gene therapy research
- Microbial infections and disease research
- Circular RNAs in diseases
- Myxozoan Parasites in Aquatic Species
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Reproductive Physiology in Livestock
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Extracellular vesicles in disease
University of the Basque Country
2015-2024
Laboratoire d'Analyses Génétiques pour les Espèces Animales
2005
Abstract Paratuberculosis is chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Whole RNA-sequencing (RNA-Seq) a promising source novel biomarkers for early MAP infection and disease progression in cattle. Since the blood transcriptome widely used as biomarkers, we analyzed whether it recapitulates, at least part, ileocecal valve (ICV), primary site colonization. Total RNA was prepared from peripheral (PB) ICV samples, RNA-Seq to compare...
In the present study, genetic analyses of diversity and differentiation were performed on four Basque-Navarrese semiferal native horse breeds. total, 417 animals genotyped for 12 microsatellite markers. Mean heterozygosity was higher than in other breeds, surely as a consequence management. Although population size some these breeds has declined appreciably past century, no bottleneck detected any possibly because it not narrow enough to be detectable. phylogenetic tree, Jaca Navarra breed...
Abstract Background Human papillomavirus (HPV) variants differ in their biological and chemical properties, therefore, may present differences pathogenicity. Most authors classified based on the phylogenetic analysis of L1 region. Nevertheless, recombination HPV samples is becoming a usual finding thus, characterizing genetic variability other regions should be essential. Objectives We aimed to characterize 18 5 genomic regions: E6, E7, E4, Upstream Regulatory Region (URR), working with both...
Endogenous retroviruses (ERVs) are the proviral phase of exogenous that become integrated into a host germ line. They can play an important role in genome. Bioinformatic tools have been used to detect ERVs several vertebrates, primarily primates and rodents. Less information is available regarding other mammalian groups, source this basically experimental. We analyzed genome cow (Bos taurus) using three different methods. A BLAST-based method detected 928 possible ERVs, LTR_STRUC 4,487...
Endogenous retroviruses (ERVs) are genomic elements that present in a wide range of vertebrates. Although the study ERVs has been carried out mainly humans and model organisms, recently, domestic animals have become important, some species begun to be analyzed gain further insight into ERVs. Due availability complete genomes development new computer tools, can now from genome-wide viewpoint. In addition, more experimental work is being analyze distribution, expression interplay within host...
There have been few in vivo studies on the effect of aluminum hydroxide adjuvant and its influence immune response to vaccination. In this study, lambs received a parallel subcutaneous treatment with either commercial vaccines containing or an equivalent dose compound only aim identifying activated molecular signature. Blood samples were taken from each animal at beginning end experiment PBMCs isolated. Total RNA miRNA libraries prepared sequenced mean sequencing depth 70 million 76 bp...
Endogenous retroviruses (ERVs) are genomic elements of retroviral origin that present in the genomes almost all vertebrates. In cattle, more than 13,000 related to ERVs have been detected, and based on pol gene, 24 families or groups bovine described. However, information about other bovids presence different species Bovidae family is scarce.The previously detected cattle (Bos taurus) were also zebus indicus) yaks grunniens). addition, six new families, named BoERV25 BoERV30, three Bos...
Recurrent Pregnancy Loss (RPL), also named Spontaneous Abortion (RSA), is a common fertility problem that refers to at least two consecutive pregnancy losses and affects 1–2% of couples all over the world. Despite causes such as genetic abnormalities, uterine anomalies or hormonal metabolic disorders, there still huge challenge in identifying about 40–60% RPL patients. Circular RNAs (circRNAs) are endogenous ncRNAs with unique closed-loop single-stranded structure. Accumulated evidence...
MicroRNAs (miRNAs) are short endogenous, single-stranded, noncoding small RNA molecules of approximately 22 nucleotides in length. They regulate gene expression posttranscriptionally by silencing mRNA expression, thus orchestrating many physiological processes. The Small Ruminant Lentiviruses (SRLV) group includes the Visna Maedi Virus (VMV) and Caprine Arthritis Encephalitis (CAEV) viruses, which cause a disease sheep goats characterized pneumonia, mastitis, arthritis encephalitis. Their...
Aluminium hydroxide adjuvants are crucial for livestock and human vaccines. Few studies have analysed their effect on the central nervous system in vivo. In this work, lambs received three different treatments of parallel subcutaneous inoculations during 16 months with aluminium-containing commercial vaccines, an equivalent dose aluminium or mock injections. Brain samples were sequenced by RNA-seq miRNA-seq expression analysis mRNAs, long non-coding RNAs microRNAs comparisons made. Although...
Circular RNAs (circRNAs) are covalently closed circular non-coding RNAs. Due to their structure, circRNAs more stable and have longer half-lives than linear making them good candidates for disease biomarkers. Despite the scientific relevance of these molecules, study in non-model organisms is still its infancy. Here, we analyse total RNA-seq data identify sheep from peripheral blood mononuclear cells (PBMCs) parietal lobe cortex. Out 2510 3403 detected cortex PBMCs, a 1379 novel were...
Long non-coding RNAs (lncRNAs) are involved in several biological processes, including the immune system response to pathogens and vaccines. The annotation functional characterization of lncRNAs is more advanced humans than livestock species. Here, we take advantage increasing number high-throughput experiments deposited public databases order uniformly analyse, profile unannotated integrate 422 ovine RNA-seq samples from system. We identified 12302 lncRNA genes with support independent...
Abstract The variability of the sheep major histocompatibility complex (MHC) class II DRB1 locus has been analyzed in this work. Exon 2 Ovar ‐DRB1 was amplified by polymerase chain reaction (PCR) with primers designed Amills et al. for goats. In a total 187 Latxa breed, we identified PCR‐single‐strand conformation polymorphism (SSCP) 19 alleles, eight them previously unpublished. Moreover, observed heterozygosity reached 91–95%. A new allelic type named DRB*14 defined, which brings to light...