A5102974087- Herpesvirus Infections and Treatments
- HIV Research and Treatment
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Vector-Borne Animal Diseases
- Orthopedic Infections and Treatments
- Bacterial biofilms and quorum sensing
- T-cell and Retrovirus Studies
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Rabbits: Nutrition, Reproduction, Health
- Ruminant Nutrition and Digestive Physiology
- Milk Quality and Mastitis in Dairy Cows
- bioluminescence and chemiluminescence research
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Prion Diseases and Protein Misfolding
- Virology and Viral Diseases
- Hepatitis B Virus Studies
- Pharmacological Effects of Natural Compounds
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Hepatitis Viruses Studies and Epidemiology
Gobierno de Navarra
2007-2017
Agrobiotechnology Institute
2007-2017
Universidad de Navarra
2005-2017
Universidad de Zaragoza
1987-2015
Consejo Superior de Investigaciones Científicas
1999-2015
Universidad Publica de Navarra
2002-2013
Gobierno de Aragón
2005
Agricultural Research Service
1999
Centro de Investigación y Tecnología Agroalimentaria de Aragón
1997-1999
Four slime-producing isolates of Staphylococcus aureus were used in an antibiotic susceptibility assay for biofilms developed on 96-well polystyrene tissue culture plates. The study involved 11 antibiotics, two biofilm ages (6 and 48 h), growth media (tryptone soy broth (TSB) delipidated milk) three concentrations (4 × MBC, 100 mg/L 500 mg/L). ATP-bioluminescence was automated bacterial viability determination after a 24 h exposure to avoid handling. Under the conditions applied, untreated...
ABSTRACT Lentivirus infections in small ruminants represent an economic problem affecting several European countries with important sheep-breeding industries. Programs for control and eradication of these are being initiated require reliable screening assays. This communication describes the construction evaluation a new serological enzyme-linked immunosorbent assay (ELISA) detection antibodies to maedi-visna virus (MVV) sheep caprine arthritis encephalitis (CAEV) goats. The solid phase is...
Abstract Staphylococcus aureus biofilms formed on medical implants represent a serious problem, being difficult to eradicate with antibiotic therapy and leading chronic infections. Simplified in vivo vitro susceptibility assays using biofilm bacteria are needed. In this work, novel osteomyelitis infection model was developed rats the absence of bacterial suspension, requiring use only 10 6 at site surgery, full success reproducing infection. Stainless‐steel pre‐colonized for 12 h highly...
Abstract Background A central nervous system (CNS) disease outbreak caused by small ruminant lentiviruses (SRLV) has triggered interest in Spain due to the rapid onset of clinical signs and relevant production losses. In a previous study on this outbreak, role LTR tropism was unclear env encoded sequences, likely involved tropism, were not investigated. This aimed analyze heterogeneity SRLV Env regions - TM amino terminal SU V4, C4 V5 segments order assess virus compartmentalization CNS....
The aim of this work was to investigate whether an enzyme-linked immunosorbent assay (ELISA) useful for early detection maedi-visna virus (MVV) infection in sheep under field conditions. An ELISA based on p25 recombinant protein and a gp46 synthetic peptide used. Sequentially obtained serum samples ( n = 1,941) were studied 4 years. results compared with those the agar gel immunodiffusion (AGID) test, both tests reference result established using consensus scores at least 2 3 serologic...
ABSTRACT The restrictive properties of tripartite motif-containing 5 alpha (TRIM5α) from small ruminant species have not been explored. Here, we identify highly similar TRIM5α sequences in sheep and goats. Cells transduced with ovine effectively restricted the lentivirus visna/maedi virus DNA synthesis. Proteasome inhibition cells restored viral synthesis, suggesting a conserved mechanism restriction. Identification active molecular may open new prophylactic strategies against lentiviral infections.