- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Effects of Environmental Stressors on Livestock
- Ovarian function and disorders
- Sperm and Testicular Function
- Renal and related cancers
- Pluripotent Stem Cells Research
- Reproductive Health and Technologies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fish Ecology and Management Studies
- Animal health and immunology
- Birth, Development, and Health
- Bioenergy crop production and management
- Bacterial biofilms and quorum sensing
- Veterinary Equine Medical Research
- Sustainable Agricultural Systems Analysis
- bioluminescence and chemiluminescence research
- Genetic diversity and population structure
- Prion Diseases and Protein Misfolding
- Bacteriophages and microbial interactions
- Genetic Mapping and Diversity in Plants and Animals
- Milk Quality and Mastitis in Dairy Cows
- Cancer-related molecular mechanisms research
Universidad de Zaragoza
1984-2023
Centro de Investigación y Tecnología Agroalimentaria de Aragón
2014-2023
Gobierno de Aragón
2017-2021
Consejo Superior de Investigaciones Científicas
1999
Four slime-producing isolates of Staphylococcus aureus were used in an antibiotic susceptibility assay for biofilms developed on 96-well polystyrene tissue culture plates. The study involved 11 antibiotics, two biofilm ages (6 and 48 h), growth media (tryptone soy broth (TSB) delipidated milk) three concentrations (4 × MBC, 100 mg/L 500 mg/L). ATP-bioluminescence was automated bacterial viability determination after a 24 h exposure to avoid handling. Under the conditions applied, untreated...
Summary Bone morphogenetic protein 15 (BMP15) is a member of the transforming growth factor beta superfamily, specifically expressed in oocytes and essential for sheep prolificacy. Reported mutations this gene cause increased ovulation rate infertility dosage‐sensitive manner. In work, new naturally occurring mutation BMP15 from ovine Rasa Aragonesa breed described. This deletion 17 bp that leads to an altered amino acid sequence introduces premature stop codon protein. Highly significant...
Antigen(s) immunologically related to pregnancy-associated glycoproteins (PAGs) have previously been detected in the serum of pregnant goats. In this work, we describe a partial characterization family PAGs isolated from placenta goat. The procedure, monitored by RIA, included extraction proteins at neutral pH, acidic, and ammonium sulfate precipitations; gel filtration ion exchange chromatographies. Immunoreactivity, initially located acidic supernatant 40-80% fractions, was equally...
Anti-Müllerian hormone (AMH) is produced by the granulosa cells (GCs) of growing follicles and inhibits follicular development. This study aimed to investigate regulation AMH-specific type 2 receptor (AMHR2) gene expression in GCs bone morphogenetic protein (BMP)15, BMP4 growth differentiation factor (GDF)9. Their effects on AMHR2 AMH mRNAs were studied luteinized human ovine (oGCs) from small antral follicles. The BMPs promoter reporter activities analyzed transfected oGCs. vivo effect...
In mammals, the ovarian follicular reserve is highly variable between individuals and impacts strongly on function fertility. Nowadays, best endocrine marker of this in human, mouse cattle anti-Müllerian hormone (AMH). The objectives work were to determine whether AMH could be detected plasma prepubertal ewe lambs assess its relationship with their fertility at a young age.Plasma was taken from 76 Rasa Aragonesa 3.6 months age for determination. Simultaneously, 600 IU equine chorionic...
The aim of this work is to establish the relationship between morphology Intracytoplasmic Morphologically Selected Sperm Injection (IMSI)-selected spermatozoa and their DNA integrity. 45 ejaculates were randomly distributed into three treatment groups: normozoospermic, oligoasthenozoospermic oligoasthenotheratozoospermic samples. evaluation integrity was performed using sperm chromatin dispersion test. It established that strongly dependent on ejaculate quality (P < 0.05). count with...
The aim of our investigation was to compare the ultrastructure lipid droplets, and effect cooling on intracellular vesicles bovine porcine GV oocytes. droplets in oocytes have a homogeneous structure. utilization lipids takes place directly from these without formation interim compounds. In contrast, there are two kinds oocytes: ‘dark’, next ‘grey’ with electron‐lucent streaks. Vesicles each specific group connected other. After 12‐h culture, cisternal smooth endoplasmic reticulum layer...
The MIC and the highest concentration enabling bacterial growth (CEG) of antibiotics contained in two selective media were determined for Brucella abortus, B. melitensis, ovis. nalidixic acid bacitracin Farrell's medium responsible inhibitory effects observed.
Abstract The aim of this study was to evaluate the effect rosemary essential oils ( REO ) and forage nature on ewes' performances, immune response lambs' growth mortality. Forty‐eight dairy ewes (Sicilo‐Sarde) were fed oat‐hay or oat‐silage supplemented with 400 g concentrate during pregnancy 600 postpartum. experimental contained same mixture as control (barley, soybean meal mineral vitamin supplement) more 0.6 g/kg . Two groups obtained each (Hay groups: H ‐ C ; Silage S ). increased dry...
A new mutation in the bone morphogenetic protein 15 (BMP15) gene (FecX(R) allele) causing increased prolificacy heterozygous (R+) and sterility homozygous ewes has been recently described Rasa Aragonesa, a low-prolificacy Mediterranean breed. The current study determined, first, effect of this polymorphism on natural eCG-induced ovulation rate (OR) eCG dose reproductive performance; second, its interaction with progestagen + treatment farms, which have not reported to date. FecX(R) allele OR...
Abstract Iberian wild goats ( Capra pyrenaica , also known as ibex, Spanish and goat) underwent strong genetic bottlenecks during the 19 th 20 centuries due to overhunting habitat destruction. From 1970s 1990s, augmentation translocations were frequently carried out restock goat populations (very often with hunting purposes), but they not systematically planned or recorded. On other hand, recent data suggest occurrence of hybridization events between domestic hircus ). Augmentation...