A5016701363- Sexual Differentiation and Disorders
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- Sperm and Testicular Function
- Urologic and reproductive health conditions
- Genetic Syndromes and Imprinting
- Testicular diseases and treatments
- Prenatal Screening and Diagnostics
- Renal and related cancers
- TGF-β signaling in diseases
- Growth Hormone and Insulin-like Growth Factors
- Hormonal and reproductive studies
- Animal Genetics and Reproduction
- Monoclonal and Polyclonal Antibodies Research
- Urological Disorders and Treatments
- Gestational Trophoblastic Disease Studies
- Cancer, Hypoxia, and Metabolism
- Ovarian cancer diagnosis and treatment
- Blood groups and transfusion
- Protease and Inhibitor Mechanisms
- Parvovirus B19 Infection Studies
- Lysosomal Storage Disorders Research
- Cholinesterase and Neurodegenerative Diseases
- Connective tissue disorders research
Inserm
2010-2023
Centre de Recherche Saint-Antoine
1995-2023
Sorbonne Université
1990-2023
Unité de recherche sur les maladies cardiovasculaires et métaboliques
2019
Délégation Paris 7
2014-2017
Sorbonne Paris Cité
2014-2017
Université Paris Cité
2005-2017
Physiologie De L'Axe Gonadotrope
2014-2016
Centre National de la Recherche Scientifique
2014-2016
Tokyo Medical and Dental University
2014
Anti-Müllerian hormone (AMH) has been detected by RIA in the follicular fluid of mature bovine ovaries and incubation medium granulosa cells. Purification AMH from two independent batches was achieved with a yield 11% 15% respectively. Both ovarian control testicular produced near-complete regression fetal rat Müllerian ducts exposed to it culture at final concentration 200-300 mU/ml were recognized same monoclonal polyclonal antibodies. These findings indicate that adult mammalian cells are...
Anti-Müllerian hormone, also called Müllerian-inhibiting substance or factor, is a glycoprotein dimer belonging to the transforming growth factor-beta superfamily and synthesized by immature Sertoli cells postnatal granulosa cells. hormone plays key role in sex differentiation inducing regression of Müllerian ducts male fetus. It responsible for stunting masculinization fetal ovaries bovine freemartin fetuses may be involved control follicular maturation ovary. Using degenerate probe...
The gene coding for human anti-Müllerian hormone (AMH) was localized to subbands p13.2----p13.3 on chromosome 19, using in situ hybridization and Southern blot analysis of a panel man-mouse man-hamster somatic cell hybrids.
In mammals, anti-Müllerian hormone (AMH) is produced by Sertoli cells from the onset of testicular differentiation and granulosa only after birth. SOX9, a transcription factor related to testis-determining SRY, expressed in mouse testis 1 day before AMH. To determine relationship between AMH SOX9 birds, we cloned promoter search response elements, compared expression gonads chick embryos using situ hybridization. Potential SOX elements were found promoter; however, both sexes at stage 25,...
Context: Puberty is associated with increased testicular testosterone (TT) synthesis, which required to trigger spermatogenesis and repress anti-Mullerian hormone (AMH) production. However, gonadotropin stimulation during fetal newborn life neither initiates nor represses AMH.
TGF-β family ligands are translated as prepropeptide precursors and processed into mature C-terminal dimers that signal by assembling a serine/threonine kinase receptor complex containing type I II components. Many secreted in latent form cannot bind their receptor, due to the pro-region remaining associated with ligand noncovalent after proteolytic cleavage. Here we show anti-Müllerian hormone (AMH), involved reproductive development, must be cleaved its (AMHRII), but dissociation of from...
In mammals, anti-Müllerian hormone (AMH) is produced by Sertoli cells from the onset of testicular differentiation and granulosa after birth. birds, AMH starts to be expressed in indifferent gonads both sexes at a similar level later up-regulated males. We previously demonstrated that, unlike expression occurs chick embryo absence SOX9. looked for potential factors that might involved regulating different stages gonad comparing its pattern embryos young chicken with DMRT1, SF-1, WT1, GATA-4,...
Granulosa cell tumors (GCT) are ovarian neoplasms that tend to recur and spread in the pelvis abdomen several years after initial treatment. Anti-Müllerian hormone (AMH) is a reliable serum marker of these tumors. To enhance availability sensitivity AMH determination, we developed an ultrasensitive enzyme-linked immunosorbent assay. In this work compare results levels, obtained using traditional assays, 31 patients with GCT followed up for 7 yr. The assay has significantly higher than one....
The persistent Müllerian duct syndrome (PMDS) is characterized by the persistence of derivatives, uterus and tubes, in otherwise normally virilized males. In a previous study, we showed that this heterogeneous, with lack production anti-Müllerian hormone (AMH) testicular tissue accounting for only some, AMH-negative, cases disorder. We have point mutation responsible an AMH-negative PMDS three siblings: guanine to thymine transversion at position 2096 fifth exon changes GAA triplet, coding...
Anti-Müllerian hormone (AMH) production by testicular Sertoli cells is high before puberty and can be further induced FSH. Our objective was to delineate the mechanisms which FSH stimulates AMH production. Assay of serum levels histological morphometric analysis in prepubertal FSH-deficient transgenic mice showed that mass were decreased owing reduced cell number. All parameters resumed normal values treated with recombinant We also analyzed ability factors involved its signaling pathway...
Anti-müllerian hormone (AMH) is a member of the TGF-β family, which limits follicle maturation. Recently serum AMH has been recognized as useful diagnostic and prognostic tool in human reproductive endocrinology.The aim this study was to investigate regulation ovarian by estradiol FSH.AMH mRNA were quantified real time RT-PCR granulosa cells (GC). transcription studied KK1 GC cotransfected with estrogen receptors (ER)-β or ERα, normal promoter-luciferase construct (hAMH-luc) mutated promoter...
Development of follicular cysts is a frequent ovarian dysfunction in cattle. Functional changes that precede cyst formation are unknown, but role for anti-Müllerian hormone (AMH) the development has been suggested humans. This study aimed to characterize intrafollicular steroids and AMH during growth strain beef cows exhibiting high incidence occurrence cysts. Normal were assessed by ultrasonography scanning 8 days before slaughtering. Experimental regression was followed rapid follicles...
STUDY QUESTIONAre anti-Müllerian hormone (AMH) and AMH type II receptor (AMHR-II) mRNAs similarly regulated by gonadotrophins in lutein granulosa cells (GCs) from control, normo-ovulatory oligo/anovulatory women with polycystic ovary syndrome (PCOS)?
The function of AMH (Anti-Müllerian hormone), a phylogenetically ancient member the TGFβ family proteins, in lower vertebrates is largely unknown. Previously, we have shown that gene encoding type II anti-Müllerian hormone receptor, amhrII, responsible for excessive germ cell proliferation and male-to-female sex reversal medaka hotei mutant. In this study, functional analyses cultured cells other amhrII mutant alleles indicate lack signaling causes phenotype. BrdU incorporation experiments...
Male patients with an extra sex chromosome or autosome are expected to present primary hypogonadism at puberty owing meiotic germ-cell failure. Scarce information is available on trisomy 21, a frequent autosomal aneuploidy. Our objective was assess whether 21 presents pubertal-onset, specific, in males, the established earlier and affects other testicular cell populations. We assessed functional status of pituitary-testicular axis, especially Sertoli function, 117 boys (ages: 2 months–20...
In Sertoli cells, anti-Müllerian hormone (AMH) expression is upregulated by FSH via cyclic AMP (cAMP), although no classical cAMP response elements exist in the AMH promoter. The to involves NF-κB and AP2; however, targeted mutagenesis of their binding sites promoter do not completely abolish response. this work we assessed whether SOX9, SF1, GATA4, AP1 might represent alternative pathways involved cAMP-mediated upregulation, using real-time RT-PCR (qPCR), mutagenesis, luciferase assays,...
ABSTRACT Regression of the Müllerian duct in male embryo is one unequivocal effect anti-Müllerian hormone, a glycoprotein secreted by Sertoli cells testis. This hormone induces ductal epithelial regression through paracrine mechanism originating periductal mesenchyme. To probe mechanisms action we have studied sequence cellular and molecular events involved regression. Studies were performed rat embryos transgenic mice overexpressing or lacking both vivo vitro Anti-Müllerian causes cranial...