A5039335985- Reproductive Biology and Fertility
- Ovarian function and disorders
- Genetic Syndromes and Imprinting
- Sexual Differentiation and Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- TGF-β signaling in diseases
- Sperm and Testicular Function
- Growth Hormone and Insulin-like Growth Factors
- Renal and related cancers
- Reproductive Physiology in Livestock
- Hormonal and reproductive studies
- Animal Genetics and Reproduction
- Kruppel-like factors research
- Prenatal Screening and Diagnostics
- Ovarian cancer diagnosis and treatment
- Cancer, Hypoxia, and Metabolism
- Testicular diseases and treatments
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Lipid metabolism and disorders
- Mesenchymal stem cell research
- Hypothalamic control of reproductive hormones
- Urologic and reproductive health conditions
- Assisted Reproductive Technology and Twin Pregnancy
- Soft tissue tumor case studies
Inserm
2014-2025
Sorbonne Université
2017-2025
Centre de Recherche Saint-Antoine
2018-2025
Unité de recherche sur les maladies cardiovasculaires et métaboliques
2019-2020
Hôpital Saint-Antoine
2019
Institut National de Recherche en Santé Publique
2007-2019
Sorbonne Paris Cité
2014-2017
Université Paris Cité
2005-2017
Délégation Paris 7
2014-2017
Unit of Functional and Adaptive Biology
2015-2017
Anti-Müllerian hormone, also called Müllerian-inhibiting substance or factor, is a glycoprotein dimer belonging to the transforming growth factor-beta superfamily and synthesized by immature Sertoli cells postnatal granulosa cells. hormone plays key role in sex differentiation inducing regression of Müllerian ducts male fetus. It responsible for stunting masculinization fetal ovaries bovine freemartin fetuses may be involved control follicular maturation ovary. Using degenerate probe...
The major limitation to the development of embryo production in cattle is strong between-animal variability ovulatory response FSH-induced superovulation, mainly due differences ovarian activity at time treatment. This study aimed establish whether anti-Müllerian hormone (AMH) was an endocrine marker follicular populations cow, as human, and a possible predictor superovulation. Anti-Müllerian concentrations plasma varied 10-fold between cows before treatment were found be highly correlated...
Strong overexpression of anti-Müllerian hormone (AMH) in transgenic mice leads to incomplete fetal virilization and decreased serum testosterone the adult. Conversely, AMH-deficient exhibit Leydig cell hyperplasia. To probe mechanism action AMH on steroidogenesis, we have studied expression mRNA for steroidogenic proteins vivo vitro performed a morphometric analysis testicular tissue overexpressing hormone. We show that male blocks differentiation precursors. Expression protein mRNAs, mainly...
In the polycystic ovary syndrome (PCOS), in addition to intrinsic thecal dysregulation leading hyperandrogenism, a granulosa cell (GC) may occur. Expression of anti-Müllerian hormone (AMH), FSH receptor (FSHR) and androgen (AR) are suspected be altered PCOS GCs.The aim this prospective study was analyze expression these genes at last stages follicular maturation GCs from 17 patients with 15 controls undergoing controlled ovarian hyperstimulation during cycle vitro fertilization.On day oocyte...
The strong relationship between serum anti-Müllerian hormone (AMH) levels and the number of antral follicles supports use AMH measurements as a quantitative marker ovarian follicular status. Yet, it still is unclear whether aptitude an individual follicle to produce reflects its reproductive competence.This study examined possible or fluid (FF) concentrations fate ensuing oocytes embryos obtained by in vitro fertilization-embryo transfer conducted monodominant cycles.We prospective at...
TGF-β family ligands are translated as prepropeptide precursors and processed into mature C-terminal dimers that signal by assembling a serine/threonine kinase receptor complex containing type I II components. Many secreted in latent form cannot bind their receptor, due to the pro-region remaining associated with ligand noncovalent after proteolytic cleavage. Here we show anti-Müllerian hormone (AMH), involved reproductive development, must be cleaved its (AMHRII), but dissociation of from...
STUDY QUESTIONAre anti-Müllerian hormone (AMH) and AMH type II receptor (AMHR-II) mRNAs similarly regulated by gonadotrophins in lutein granulosa cells (GCs) from control, normo-ovulatory oligo/anovulatory women with polycystic ovary syndrome (PCOS)?
ABSTRACT Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting substance or factor, has previously been shown to sex-reverse the steroidogenic pattern of fetal mammalian ovaries through repression aromatase biosynthesis. Study ontogeny response cyclic AMP-stimulated activity rat AMH allowed us develop a quantitative biossay for hormone. Linear responses function logarithm concentration were observed over ranges 0.2 -7.5 μg/ml bovine protein and 0.15 -2 human protein, with maximal...
Mullerian inhibiting substance (MIS)/anti-Mullerian hormone is a differentiation factor that causes regression of the duct in developing male fetus and an apparent sex reversal fetal ovary when inappropriately exposed to it. The purified product 140-kilodalton glycoprotein composed two identical subunits. We show C-terminal fragment MIS, which shares homology with transforming growth factor-beta, inhibits biosynthesis aromatase ovary. However, both activities are enhanced dramatically by...
Anti-müllerian hormone (AMH) is a member of the TGF-β family, which limits follicle maturation. Recently serum AMH has been recognized as useful diagnostic and prognostic tool in human reproductive endocrinology.The aim this study was to investigate regulation ovarian by estradiol FSH.AMH mRNA were quantified real time RT-PCR granulosa cells (GC). transcription studied KK1 GC cotransfected with estrogen receptors (ER)-β or ERα, normal promoter-luciferase construct (hAMH-luc) mutated promoter...
Development of follicular cysts is a frequent ovarian dysfunction in cattle. Functional changes that precede cyst formation are unknown, but role for anti-Müllerian hormone (AMH) the development has been suggested humans. This study aimed to characterize intrafollicular steroids and AMH during growth strain beef cows exhibiting high incidence occurrence cysts. Normal were assessed by ultrasonography scanning 8 days before slaughtering. Experimental regression was followed rapid follicles...
The function of AMH (Anti-Müllerian hormone), a phylogenetically ancient member the TGFβ family proteins, in lower vertebrates is largely unknown. Previously, we have shown that gene encoding type II anti-Müllerian hormone receptor, amhrII, responsible for excessive germ cell proliferation and male-to-female sex reversal medaka hotei mutant. In this study, functional analyses cultured cells other amhrII mutant alleles indicate lack signaling causes phenotype. BrdU incorporation experiments...
In Sertoli cells, anti-Müllerian hormone (AMH) expression is upregulated by FSH via cyclic AMP (cAMP), although no classical cAMP response elements exist in the AMH promoter. The to involves NF-κB and AP2; however, targeted mutagenesis of their binding sites promoter do not completely abolish response. this work we assessed whether SOX9, SF1, GATA4, AP1 might represent alternative pathways involved cAMP-mediated upregulation, using real-time RT-PCR (qPCR), mutagenesis, luciferase assays,...
Anti-Müllerian hormone (AMH) contributes to male sexual differentiation and acts on gonads of both sexes. Identification AMH receptivity in pituitary brain has led the intriguing idea that participates hypothalamic-pituitary control reproduction, however vivo experimental evidence is still lacking. We show stimulates secretion gene expression gonadotropin FSH rats. action sex-dependent, being restricted females occurring before puberty. Accordingly, we report higher levels receptor...
ABSTRACT Regression of the Müllerian duct in male embryo is one unequivocal effect anti-Müllerian hormone, a glycoprotein secreted by Sertoli cells testis. This hormone induces ductal epithelial regression through paracrine mechanism originating periductal mesenchyme. To probe mechanisms action we have studied sequence cellular and molecular events involved regression. Studies were performed rat embryos transgenic mice overexpressing or lacking both vivo vitro Anti-Müllerian causes cranial...