A5041544474- Ovarian function and disorders
- Reproductive Biology and Fertility
- Ovarian cancer diagnosis and treatment
- Sexual Differentiation and Disorders
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Hypothalamic control of reproductive hormones
- Hormonal and reproductive studies
- Growth Hormone and Insulin-like Growth Factors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Health and Contraception
- Sexual function and dysfunction studies
- Assisted Reproductive Technology and Twin Pregnancy
- Hormonal Regulation and Hypertension
- Reproductive System and Pregnancy
- Testicular diseases and treatments
- Estrogen and related hormone effects
- LGBTQ Health, Identity, and Policy
- Menopause: Health Impacts and Treatments
- Birth, Development, and Health
- Endometriosis Research and Treatment
- Prenatal Screening and Diagnostics
- BRCA gene mutations in cancer
- Gestational Diabetes Research and Management
- Regulation of Appetite and Obesity
Centre Hospitalier Universitaire de Lille
2015-2024
Université de Lille
2013-2024
Hôpital Jeanne de Flandre
2015-2024
Centre de Recherche Jean Pierre Aubert
2015-2024
Lille Neurosciences & Cognition
2019-2024
Inserm
2015-2023
Hôpital Albert Calmette
2022
Université Lille Nord de France
2013-2016
Physiologie de la Reproduction et des Comportements
2012
Polycystic ovarian morphology (PCOM) at ultrasound is currently used in the diagnosis of polycystic ovary syndrome (PCOS). We hypothesized that previously proposed threshold value 12 as an excessive number follicles per (FN) no longer appropriate because current technological developments. In this study, we have revisited thresholds for FN and serum Anti-Müllerian hormone (AMH) level (a possible surrogate FN) definition PCOM.Clinical, hormonal data were consecutively recorded 240 patients...
Abstract Anti-Müllerian hormone (AMH) plays crucial roles in sexual differentiation and gonadal functions. However, the possible extragonadal effects of AMH on hypothalamic–pituitary–gonadal axis remain unexplored. Here we demonstrate that a significant subset GnRH neurons both mice humans express receptor, potently activates neuron firing mice. Combining vivo vitro experiments, show increases GnRH-dependent LH pulsatility secretion, supporting central action neurons. Increased is an...
Polycystic ovary syndrome (PCOS) is the most common reproductive and metabolic disorder affecting women of age. PCOS has a strong heritable component, but its pathogenesis been unclear. Here, we performed RNA sequencing genome-wide DNA methylation profiling ovarian tissue from control third-generation PCOS-like mice. We found that hypomethylation regulates key genes associated with several differentially methylated are also altered in blood samples compared healthy controls. Based on this...
In the polycystic ovary syndrome (PCOS), in addition to intrinsic thecal dysregulation leading hyperandrogenism, a granulosa cell (GC) may occur. Expression of anti-Müllerian hormone (AMH), FSH receptor (FSHR) and androgen (AR) are suspected be altered PCOS GCs.The aim this prospective study was analyze expression these genes at last stages follicular maturation GCs from 17 patients with 15 controls undergoing controlled ovarian hyperstimulation during cycle vitro fertilization.On day oocyte...
By requiring a minimum of two three items [hyperandrogenism (HA), oligoanovulation (OA), and polycystic ovaries (PCO) at ultrasound], the Rotterdam definition recognizes four PCO syndrome (PCOS) phenotypes: HA+OA+PCO (full-blown syndrome), HA+OA (former National Institutes Health definition), HA+PCO (ovulatory PCOS), OA+PCO. However, latter phenotype is controversial, it not known to what extent shares similarities with others.The study was comparative analysis hormonal, metabolic,...
It is still debated whether clinical and/or biological indices of hyperandrogenism (HA) should be present to qualify a patient as having polycystic ovary syndrome (PCOS). We hypothesized that excessive follicle number (FN) assessed by ovarian ultrasonography serum anti-Müllerian hormone (AMH) concentrations may used surrogates for the classical markers HA.Data were obtained from database clinical, hormonal, and ultrasound features consecutively recorded in 270 women with PCOS (defined using...
Recombinant human GH treatment and oocyte donation (OD) have improved the quality of life in women with Turner syndrome (TS). However, expectancy is reduced, mainly due to cardiovascular complications. Pregnancy may itself increase that risk be associated hazardous materno-fetal outcome.The objective this study was evaluate outcome ongoing pregnancies beyond 20 wk gestation obtained by OD TS.This a multicenter retrospective including all assisted reproductive technology centers affiliated...
STUDY QUESTIONAre anti-Müllerian hormone (AMH) and AMH type II receptor (AMHR-II) mRNAs similarly regulated by gonadotrophins in lutein granulosa cells (GCs) from control, normo-ovulatory oligo/anovulatory women with polycystic ovary syndrome (PCOS)?
Primary Ovarian Insufficiency (POI), a public health problem, affects 1-3.7% of women under 40 yielding infertility and shorter lifespan. Most causes are unknown. Recently, genetic were identified, mostly in single families. We studied an unprecedented large cohort POI to unravel its molecular pathophysiology.375 patients with 70 families using targeted (88 genes) or whole exome sequencing pathogenic/likely-pathogenic variant selection. Mitomycin-induced chromosome breakages patients'...
Anti-müllerian hormone (AMH) is a member of the TGF-β family, which limits follicle maturation. Recently serum AMH has been recognized as useful diagnostic and prognostic tool in human reproductive endocrinology.The aim this study was to investigate regulation ovarian by estradiol FSH.AMH mRNA were quantified real time RT-PCR granulosa cells (GC). transcription studied KK1 GC cotransfected with estrogen receptors (ER)-β or ERα, normal promoter-luciferase construct (hAMH-luc) mutated promoter...
It is not known whether polycystic ovaries (PCO) are an ovarian appearance without pathological meaning or they share with ovary syndrome (PCOS) the same follicle abnormality. There few studies including strictly selected women PCO but other criteria of PCOS. In order to address these issues, we compared hormonal, metabolic and ultrasound parameters obtained from patients only, PCOS controls.This was a comparative analysis three age-matched groups 95 patients, who were included consecutively...
Does the use of serum anti-Müllerian hormone (AMH) assay to replace or complement ultrasound (U/S) affect diagnosis phenotypic distribution polycystic ovary syndrome (PCOS)?Combining U/S and AMH define ovarian morphology (PCOM) diagnoses PCOS (according Rotterdam classification) in more patients than definitions using one other these indicators exclusively.Since 2003, PCOM, as defined by U/S, is three diagnostic criteria for PCOS. As it closely correlated with follicle excess seen at an...
Primary ovarian insufficiency (POI) affects 1% of women under 40 years age. POI is idiopathic in more than 70% cases. Though many candidate genes have been identified recent years, the prevalence and pathogenicity abnormalities are still difficult to establish.Our primary objective was evaluate gene variations a large prospective multicentric cohort. Our secondary correlation between phenotype genotype.Two hundred sixty-nine well-phenotyped patients were screened for variants 18 known...
The nitric oxide (NO) signaling pathway in hypothalamic neurons plays a key role the regulation of secretion gonadotropin-releasing hormone (GnRH), which is crucial for reproduction. We hypothesized that disruption neuronal NO synthase (NOS1) activity underlies some forms hypogonadotropic hypogonadism. Whole-exome sequencing was performed on cohort 341 probands with congenital hypogonadism to identify ultrarare variants NOS1 . identified mutant proteins assessed by their ability promote...
We have recently demonstrated a causal link between loss of gonadotropin-releasing hormone (GnRH), the master molecule regulating reproduction, and cognitive deficits during pathological aging, including Down syndrome Alzheimer's disease. Olfactory alterations, which persist in some COVID-19 patients, long-term hypotestosteronaemia SARS-CoV-2-infected men are also reminiscent consequences deficient GnRH, suggesting that GnRH system neuroinvasion could underlie certain post-COVID symptoms...
Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance, a member of the TGF-ß family, is responsible for regression Müllerian ducts in male fetus. In females, AMH synthesized by granulosa cells preantral and small antral follicles, production wanes at later stages follicle maturation. Using RT-PCR luteal primary culture reporter gene techniques KK1 cell line, we show that FSH cAMP enhance transcription, LH has an additive effect. Gonadotropins act through protein kinase A...
Context: In the general population, about 30% of asymptomatic women have polycystic ovary-like abnormalities (PCO-L), i.e. ovarian morphology (PCOM) at ultrasound and/or increased anti-Müllerian hormone (AMH) serum level. PCOM has also been reported in 30–50% with functional hypothalamic amenorrhea (FHA). Objective: The aim this study was to verify whether both and excessive AMH level indicate PCO-L FHA elucidate its significance. Design: We conducted a retrospective analysis using database...
Abstract STUDY QUESTION What are the changes in serum concentration of total and cleaved anti-Muüllerian hormone (AMH) molecular forms androgens before throughout pregnancy women with without polycystic ovary syndrome (PCOS) a longitudinal follow-up investigation? SUMMARY ANSWER Serum levels AMH higher from preconception to third trimester PCOS as compared controls, whereas testosterone androstenedione than control during second pregnancy. WHAT IS KNOWN ALREADY Cross-sectional or partial...