A5065250369- Reproductive Biology and Fertility
- Ovarian function and disorders
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Reproductive Health and Technologies
- Pluripotent Stem Cells Research
- Endometriosis Research and Treatment
- Renal and related cancers
- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- MicroRNA in disease regulation
- Gynecological conditions and treatments
- Animal Genetics and Reproduction
- Ectopic Pregnancy Diagnosis and Management
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- Sexual Differentiation and Disorders
- Molecular Biology Techniques and Applications
- Birth, Development, and Health
- Ovarian cancer diagnosis and treatment
- Pregnancy and preeclampsia studies
- COVID-19 Impact on Reproduction
Institute for Regenerative Medicine & Biotherapy
2014-2024
Centre Hospitalier Universitaire de Montpellier
2012-2024
Université de Montpellier
2015-2024
Hôpital Arnaud de Villeneuve
2015-2024
Hôpital Saint Eloi
2014-2024
Inserm
2015-2024
Physiologie de la Reproduction et des Comportements
1983-2022
Développement Embryonnaire, Fertilité et Environnement
2015-2021
Hôpital Femme Mère Enfant
2021
Hudson Institute
2017
BACKGROUNDThe Fifth Evian Annual Reproduction (EVAR) Workshop Meeting discussed knowledge regarding contemporary genetics in female reproduction.
BACKGROUND: The understanding of the mechanisms regulating human oocyte maturation is still rudimentary. We have identified transcripts differentially expressed between immature and mature oocytes cumulus cells. METHODS: Using oligonucleotide microarrays, genome-wide gene expression was studied in pooled or cells from patients who underwent IVF. RESULTS: In addition to known genes, such as DAZL, BMP15 GDF9, up-regulated 1514 genes. show that PTTG3 AURKC are respectively securin Aurora kinase...
BACKGROUNDThe adjunction of exogenous hormones for controlled ovarian stimulation (COS) may alter endometrial receptiveness. In order to identify the genes misregulated under COS, we compared endometrium gene expression profiles, from same patients, in a natural cycle and subsequent COS cycle.
Identification of new criteria for embryo quality is required to improve the clinical outcome in vitro fertilization. The aim this study was determine gene expression profile cumulus cells (CC) surrounding oocyte as biomarkers potential and identify genes be used prognostic indicators successful pregnancy. CC from single oocytes were analysed using DNA microarrays. Gene profiles associated with good embryonic pregnancy computed. We observed that issued developed into embryos a morphology had...
Identification of new markers assessing endometrial receptivity may help in improving the clinical outcome IVF. This study aimed at identifying genes expressed human endometrium during implantation window that could be used as such markers.A series normoresponder patients (n = 31) underwent biopsies 62, 2 per patient) early secretory phase, days after LH surge (LH + 2) and mid-secretory phase 7) same natural cycle preceded a ICSI attempt for male infertility factor. Samples were analyzed...
What is the expression pattern of microRNAs (miRNAs) in human cumulus–oocyte complexes (COCs)? Several miRNAs are enriched cumulus cells (CCs) or oocytes, and predicted to target genes involved biological functions COC. The transcriptional profiles MII oocytes surrounding CCs known. However, very limited data available about post-transcriptional regulators, such as miRNAs. This first study focussing on identification quantification small RNAs, including miRNAs, using a deep-sequencing...
Cryopreservation is now considered as an efficient way to store human oocytes preserve fertility. However, little known about the effects of this technology on oocyte gene expression. The aim study was examine effect two cryopreservation procedures, slow freezing and vitrification, expression profile metaphase II (MII) oocytes. Unfertilized MII following ICSI failure were cryopreserved either by or Cryotip method for vitrification. After thawing, total RNA extracted analyzed using Affymetrix...
Abstract Circulating or “extracellular” microRNAs (miRNAs) detected in biological fluids, could be used as potential diagnostic and prognostic biomarkers of several disease, such cancer, gynecological pregnancy disorders. However, their contributions female infertility vitro fertilization (IVF) remain unknown. This study investigated the expression profiles five circulating miRNAs (let-7b, miR-29a, miR-30a, miR-140 miR-320a) human follicular fluid from 91 women with normal ovarian reserve 30...
Primary Ovarian Insufficiency (POI), a public health problem, affects 1-3.7% of women under 40 yielding infertility and shorter lifespan. Most causes are unknown. Recently, genetic were identified, mostly in single families. We studied an unprecedented large cohort POI to unravel its molecular pathophysiology.375 patients with 70 families using targeted (88 genes) or whole exome sequencing pathogenic/likely-pathogenic variant selection. Mitomycin-induced chromosome breakages patients'...
Abstract Background The first week of human pre-embryo development is characterized by the induction totipotency and then pluripotency. understanding this delicate process will have far reaching implication for in vitro fertilization regenerative medicine. Human mature MII oocytes embryonic stem (ES) cells are both able to achieve feat cell reprogramming towards pluripotency, either somatic nuclear transfer or fusion, respectively. Comparison transcriptome these two types may highlight genes...
The impact of gonadotropin-releasing hormone (GnRH) agonist long compared with GnRH antagonist protocols, under in vitro fertilization conditions on endometrial receptivity, is still debated. Therefore, we the effect both and protocols receptivity by analyzing, to our knowledge for first time, global gene expression profile shift during prereceptive receptive stages stimulated cycles two analogue natural same patients. For normal-responder patients, biopsies were collected day oocyte...
Crosstalk between human trophectoderm (TE) and endometrial cells during the implantation window is a complex not well-understood process. The aims of this study were (i) to evaluate global gene expression profile in TE from Day 5 blastocysts issued IVF, (ii) compare these data with transcriptomic stimulated cycles for IVF (iii) identify potential early dialogues maternal embryonic window. Endometrial biopsies (n = 18) normal responder patients performed on day embryo transfer (Day after...
Oocyte developmental competence is altered in patients with polycystic ovary syndrome (PCOS); gene expression cumulus cells (CCs) from mature metaphase II oocytes of PCOS as well? Compared CCs non-PCOS patients, the profile isolated present alterations that could explain abnormal folliculogenesis and reduced oocyte such patients. Abnormal mRNA several members insulin-like growth factor (IGF) family was previously reported. Moreover, whole transcriptome has been investigated cultured This...
Impact of female aging is an important issue in human reproduction. There was a need for extensive analysis age impact on transcriptome profile cumulus cells (CCs) to link oocyte quality and developmental potential with patient’s age. CCs from patients three groups were analyzed individually using microarrays. RT-qPCR validation performed independent CC cohorts. We focused here pathways affected by that may explain the decline In collected >37 years, angiogenic genes including ANGPTL4 ,...
The trophoblast cell lineage is specified early at the blastocyst stage, leading to emergence of trophectoderm and pluripotent cells inner mass. Using a double mRNA amplification technique comparison with transcriptome data on stem cells, placenta, germinal adult tissues, we report here some essential molecular features human mural trophectoderm. In addition genes known for their role in placenta (CGA, PGF, ALPPL2 ABCG2), also strongly expressed Laminins, such as LAMA1, GAGE Cancer/Testis...
Several studies suggest a decrease in sperm quality men the last decades. Therefore, aim of this work was to assess influence male factors (sperm and paternal age) on outcomes conventional vitro fertilization (IVF) intracytoplasmic injection (ICSI). This retrospective study included all couples who underwent IVF or ICSI at Montpellier University Hospital, France, between 1 January 2010 31 December 2015. Exclusion criteria were cycles using surgically retrieved frozen sperm, with...