The expression of GLUT-1 (erythroid/Hep G2) and GLUT-4 (muscle/fat) glucose transporters was assessed during development in rat heart, skeletal muscle, brown adipose tissue. protein detectable fetal heart by day 21 pregnancy; it increased progressively after birth, attaining levels close to those adults at 15 post natal. In contrast, messenger RNA (mRNA) already present hearts from 17 day-old fetuses. mRNA stayed low early postnatal life tissue only 10 pattern for muscle comparable that...

New advances in high-throughput technologies have allowed for the massive analysis of genomic data, providing new opportunities characterization transcriptome architectures. Recent studies pigs employed RNA-Seq to explore different tissues a reduced number animals. The main goal this study was identification differentially-expressed genes liver Iberian x Landrace crossbred showing extreme phenotypes intramuscular fatty acid composition using RNA-Seq. transcriptomes two female groups (H and...

The aim of this work is to better understand the genetic mechanisms determining two complex traits affecting porcine meat quality: intramuscular fat (IMF) content and its fatty acid (FA) composition. With purpose, expression Genome-Wide Association Study (eGWAS) 45 lipid-related genes associated with quality in swine muscle (Longissimus dorsi) 114 Iberian × Landrace backcross animals was performed. eGWAS identified 241 SNPs 11 genes: ACSM5, CROT, FABP3, FOS, HIF1AN, IGF2, MGLL, NCOA1,...

Abstract The gut microbiota has been evolving with its host along the time creating a symbiotic relationship. In this study, we assess role of genome in modulation composition pigs. Gut compositions were estimated through sequencing V3-V4 region 16S rRNA gene from rectal contents 285 A total 1,261 operational taxonomic units obtained and grouped 18 phyla 101 genera. Firmicutes (45.36%) Bacteroidetes (37.47%) two major obtained, whereas at genus level Prevotella (7.03%) Treponema (6.29%) most...

The gut microbiota is a key player in the host metabolism. Some bacteria are able to ferment non-digestible compounds and produce short-chain fatty acids that can later transform accumulate tissue. In this study, we aimed better understand relationships between microorganisms acid composition of rectal content, including possible linkage with backfat muscle pig. We studied Duroc × Iberian crossbred population, found significant correlations different bacterial archaeal genera profile....

Abstract Background Intramuscular fat (IMF) content and its fatty acid (FA) composition are typically controlled by several genes, each with a small effect. In the current study, to pinpoint candidate genes putative regulators involved in FA composition, we performed multivariate integrative analysis between intramuscular transcriptome profiles of porcine longissimus dorsi (LD) muscle. We also carried out combination network, regulatory impact factor (RIF), silico prediction target...

1. GLUT-4 glucose-transporter protein and mRNA levels were assessed in heart, red muscle white muscle, as well brown adipose tissue from 7-day streptozotocin-induced diabetic 48 h-fasted rats. 2. In agreement with previous data, showed a substantial decrease response to both diabetes fasting. Similarly, markedly decreased insulinopenic conditions. 3. Under control conditions, the level of expression content differed substantially skeletal muscle. Thus was maximal had greater compared spite...

In this paper, we describe a rapid and accurate real-time quantitative PCR-based system to determine transgene copy number in transgenic animals. We used the 2−ΔΔCt method analyze different lines without requirement of control sample previously determined by Southern blot analysis. To several mouse carrying goat β-Lactoglobulin transgene, developed TaqMan® assay which genomic DNA was as calibrator. Moreover, glucagon gene reference because is highly conserved between species amplifies with...

Recent studies in pigs have detected copy number variants (CNVs) using the Comparative Genomic Hybridization technique arrays designed to cover specific porcine chromosomes. The goal of this study was identify CNV regions (CNVRs) swine species based on whole genome SNP genotyping chips. We used predictions from three different programs (cnvPartition, PennCNV and GADA) analyze data Porcine SNP60 BeadChip. A total 49 CNVRs were identified 55 animals an Iberian x Landrace cross (IBMAP)...

In pigs, adipose tissue is one of the principal organs involved in regulation lipid metabolism. It particularly overall fatty acid synthesis with consequences other lipid-target such as muscles and liver. With this mind, we have used massive, parallel high-throughput sequencing technologies to characterize porcine transcriptome architecture six Iberian x Landrace crossbred pigs showing extreme phenotypes for intramuscular composition (three per group). High-throughput RNA was generate a...

The aim of this work was to study the genetic basis backfat expression lipid-related genes associated with meat quality traits in pigs. We performed a genome-wide association gene measured 44 by qPCR and PorcineSNP60 BeadChip genotypes 115 Iberian x Landrace backcross animals. A total 193 expression-associated SNPs located 19 chromosomal regions were levels ACSM5, ELOVL6, FABP4, FADS2, SLC27A4 genes. Three quantitative trail loci (eQTLs) corresponding FADS2 classified as cis-acting eQTLs,...

Understanding the molecular basis of cell function and ultimate phenotypes is crucial for development biological markers. With this aim, several RNA-seq studies have been devoted to characterization transcriptome ejaculated spermatozoa in relation sperm quality fertility. Semen follows a seasonal pattern decays summer months animal species. The aim study was deeply profile boar evaluate its changes. We sequenced total short fractions RNA from 10 Pietrain boars, 5 collected five sampled...

Abstract Meat quality has an important genetic component and can be modified by the fatty acid (FA) composition amount of fat contained in adipose tissue muscle. The present study aimed to find genomic regions associated with FA backfat muscle ( longissimus dorsi ) 439 pigs three different backgrounds but having Iberian breed common. Genome-wide association studies (GWAS) were performed between 38,424 single-nucleotide polymorphisms (SNPs) covering pig genome 60 phenotypic traits related...

Abstract We have recently isolated a renal cDNA clone (rBAT) that induces amino acid transport in oocytes either as component or specific activator of system bo,(+)-like transporter. (Bertran, J., Werner, A., Moore, M. L., Stange, G., Markovich, D., Biber, Testar, X., Zorzano, Palacin, M., and Murer, H. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 5601-5605. In order to obtain additional information on the rBAT protein, we investigated cellular localization its expression during development...

GLUT1 and GLUT4 glucose transporter expression is highly regulated in muscle adipose tissue during perinatal life. Here we have investigated the role of thyroid hormones regulation induction repression associated to neonatal development. Perinatal hypothyroidism markedly impaired protein heart. This effect was heart specific, a greater detected brown from hypothyroid rats compared with controls. These changes were not or when induced adult rats. results indicate that life sensitive both...

The lipid content and fatty acid (FA) profile have an important impact in human health as well the technological transformation nutritional organoleptic quality of meat. A genome-wide association study (GWAS) on 144 backcross pigs (25% Iberian × 75% Landrace) was performed for 32 traits associated with intramuscular FA composition indices metabolism. GWAS carried out using Qxpak 5.0 genotyping information obtained from Porcine SNP60K BeadChip (Illumina Inc., San Diego, CA). Signals...

Fatty acids (FA) play a critical role in energy homeostasis and metabolic diseases; the context of livestock species, their profile also impacts on meat quality for healthy human consumption. Molecular pathways controlling lipid metabolism are highly interconnected not fully understood. Elucidating these molecular processes will aid technological development towards improvement pork increased knowledge FA metabolism, underpinning diseases humans. The results from genome-wide association...

Abstract Circular RNAs (circRNAs) are emerging as a novel class of noncoding which potential role gene regulators is quickly gaining interest. circRNAs have been studied in different tissues and cell types across several animal species. However, thorough characterization the circRNAome ejaculated sperm remains unexplored. In this study, we profiled circRNA catalogue using 40 porcine ejaculates. A complex population 1,598 was shared at least 30 samples. Generally speaking, predicted presented...

Abstract Background Genetic pressure in animal breeding is sparking the interest of breeders for selecting elite boars with higher sperm quality to optimize ejaculate doses and fertility rates. However, molecular basis not yet fully understood. Our aim was identify candidate genes, pathways DNA variants associated swine by analysing 25 sperm-related phenotypes integrating genome-wide association studies (GWAS) RNA-seq under a systems biology framework. Results By GWAS, we identified 12...

Abstract Fatty acids (FAs) play an essential role as mediators of cell signaling and signal transduction, affecting metabolic homeostasis determining meat quality in pigs. However, FAs are transformed by the action several genes, such those encoding desaturases elongases lipogenic tissues. The aim current work was to identify candidate biological processes, pathways involved modulation intramuscular FA profile from longissimus dorsi muscle. gas chromatography methyl esters gene expression...

1. GLUT5 gene expression was studied in small intestine under a variety of conditions characterized by altered intestinal absorption monosaccharides. 2. RNA-blotting studies showed that mRNA abundantly expressed rat and rabbit kidney, but it not detected heart or brown adipose tissue. levels were higher the upper segments (duodenum proximal jejunum) than lower (distal jejunum ileum). 3. The circadian rhythm. A 12-fold increase at end light cycle beginning dark when compared with early...

Relative quantification is the strategy of choice for processing RT-qPCR data in microRNAs (miRNAs) expression studies. Normalisation relative performed by using reference genes. In livestock species, such as pigs, determination miRNAs and optimal number them has not been widely studied. this study, stability ten (Ssc-let-7a, Ssc-miR-103, Ssc-miR-17-3p, Hsa-miR-25, Hsa-miR-93, Ssc-miR-106a, Ssc-miR-191, Ssc-miR-16, Ssc-miR-26a Ssc-miR-17-5p) was investigated different tissues (skeletal...

The study of the boar sperm transcriptome by RNA-seq can provide relevant information on quality and fertility might contribute to animal breeding strategies. However, analysis spermatozoa RNA is challenging as these cells harbor very low amounts highly fragmented RNA, ejaculates also contain other cell types with larger non-fragmented RNA. Here, we describe a strategy for successful purification, extraction library preparation. Using approaches our objectives were: (i) evaluate recovery...