A5087333292- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Meat and Animal Product Quality
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Animal Nutrition and Physiology
- Animal Virus Infections Studies
- Fatty Acid Research and Health
- Molecular Biology Techniques and Applications
- Calpain Protease Function and Regulation
- Genomic variations and chromosomal abnormalities
- Lipid metabolism and biosynthesis
Universitat Autònoma de Barcelona
2014-2022
Center for Research in Agricultural Genomics
2013-2022
Consejo Superior de Investigaciones Científicas
2013-2014
The aim of this work is to better understand the genetic mechanisms determining two complex traits affecting porcine meat quality: intramuscular fat (IMF) content and its fatty acid (FA) composition. With purpose, expression Genome-Wide Association Study (eGWAS) 45 lipid-related genes associated with quality in swine muscle (Longissimus dorsi) 114 Iberian × Landrace backcross animals was performed. eGWAS identified 241 SNPs 11 genes: ACSM5, CROT, FABP3, FOS, HIF1AN, IGF2, MGLL, NCOA1,...
In pigs, adipose tissue is one of the principal organs involved in regulation lipid metabolism. It particularly overall fatty acid synthesis with consequences other lipid-target such as muscles and liver. With this mind, we have used massive, parallel high-throughput sequencing technologies to characterize porcine transcriptome architecture six Iberian x Landrace crossbred pigs showing extreme phenotypes for intramuscular composition (three per group). High-throughput RNA was generate a...
The aim of this work was to study the genetic basis backfat expression lipid-related genes associated with meat quality traits in pigs. We performed a genome-wide association gene measured 44 by qPCR and PorcineSNP60 BeadChip genotypes 115 Iberian x Landrace backcross animals. A total 193 expression-associated SNPs located 19 chromosomal regions were levels ACSM5, ELOVL6, FABP4, FADS2, SLC27A4 genes. Three quantitative trail loci (eQTLs) corresponding FADS2 classified as cis-acting eQTLs,...
Background Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore pig transcriptome with unprecedented detail. aim of this study was identify differentially-expressed genes between two groups 6 sows belonging Iberian × Landrace backcross extreme phenotypes according FA profile. Results We sequenced acquiring 787.5 M 75 bp paired-end reads. About 85.1% reads were mapped reference genome....
The ELOVL fatty acid elongase 6 (ELOVL6), the only related to de novo lipogenesis, catalyzes rate-limiting step in elongation cycle by controlling balance mammals. It is located on pig chromosome 8 (SSC8) a region where QTL affecting palmitic, and palmitoleic composition was previously detected, using an Iberian x Landrace intercross. main goal of this work fine-map evaluate ELOVL6 gene as positional candidate percentages palmitic acids pigs.The combination haplotype-based approach...
Transmission ratio distortion (TRD) is the departure from expected Mendelian in offspring, a poorly investigated biological phenomenon livestock species. Given current availability of specific parametric methods for analysis segregation data, this study focused on screening TRD 602 402 single nucleotide polymorphisms covering all autosomal chromosomes seven Spanish beef cattle breeds. On average, 0.13% (n = 786) and 0.01% 29) genetic markers evidenced sire- or dam-specific respectively....
Copy number variations (CNVs) are important genetic variants complementary to SNPs, and can be considered as biomarkers for some economically traits in domestic animals. In the present study, a genomic analysis of porcine CNVs based on next-generation sequencing data was carried out identify segregating an Iberian x Landrace backcross population study their association with fatty acid composition growth-related traits. A total 1,279 CNVs, including duplications deletions, were detected,...
In previous studies on an Iberian x Landrace cross, we have provided evidence that supported the porcine ELOVL6 gene as major causative of QTL pig chromosome 8 for palmitic and palmitoleic acid contents in muscle backfat. The single nucleotide polymorphism (SNP) ELOVL6:c.-533C > T located promoter region was found to be highly associated with expression and, accordingly, percentages acids longissimus dorsi adipose tissue. main goal current work further study role these traits by analyzing...
Background Several QTLs have been identified for major economically relevant traits in livestock, such as growth and meat quality, revealing the complex genetic architecture of these traits. The use network approaches considering interactions multiple molecules provides useful insights into molecular underpinnings Here, a based methodology, named Association Weight Matrix, was applied to study gene pathways affecting pig conformation, fatness Results co-association analysis underpinned three...
Summary APOA 2 is a protein implicated in triglyceride, fatty acid and glucose metabolism. In pigs, the gene located on pig chromosome 4 ( SSC 4) QTL region affecting composition, fatness growth traits. this study, we evaluated as candidate for meat quality traits an Iberian × Landrace backcross population. The :c.131T>A polymorphism, exon 3 of determining missense mutation, was associated with percentage hexadecenoic [C16:1(n–9)], linoleic [C18:2(n–6)], α ‐linolenic [C18:3(n–3)],...
Fat content and fatty acid composition in swine are becoming increasingly studied because of their effect on sensory nutritional quality meat. A QTL (quantitative trait locus) for backfat was previously detected porcine chromosome 8 (SSC8) an Iberian x Landrace F2 intercross. More recently, a genome-wide association study the same genomic region muscle backcross population. ELOVL6, strong positional candidate gene this QTL, contains polymorphism its promoter (ELOVL6:c.-533C < T), which is...
Summary The FABP 4 and 5 genes, coding for fatty acid transport proteins, have long been studied as positional candidate genes SSC QTL affecting fat deposition composition traits in pigs. Polymorphisms these :g.2634_2635insC :g.3000T>G, previously associated with fatness an Iberian by Landrace cross ( IBMAP ). aim of the present work was to evaluate functional implication genetic variants. For this purpose, mRNA expression levels 114 BC 1_ LD animals (25% × 75% Landrace) were analyzed...
Fat content and fatty acid (FA) composition determine important sensory technological aspects of pork meat products because their influence on the melting point oxidative status porcine tissues. Artificial selection to increase production in pigs has caused a reduction intramuscular fat changes FA some breeds. Gene expression levels can be analyzed as quantitative trait identify genomic regions regulating its expression. These are known loci (eQTLs). The detection eQTLs been proposed good...