A5055704539- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Angiogenesis and VEGF in Cancer
- Peptidase Inhibition and Analysis
- Estrogen and related hormone effects
- Proteoglycans and glycosaminoglycans research
- Mathematical Biology Tumor Growth
- Platelet Disorders and Treatments
- Ubiquitin and proteasome pathways
- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Growth Hormone and Insulin-like Growth Factors
- CAR-T cell therapy research
- Cellular Mechanics and Interactions
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Thyroid Disorders and Treatments
- Kruppel-like factors research
- Histone Deacetylase Inhibitors Research
- Blood Coagulation and Thrombosis Mechanisms
- Chronic Myeloid Leukemia Treatments
- Cancer Cells and Metastasis
- Prenatal Substance Exposure Effects
- PARP inhibition in cancer therapy
- Axon Guidance and Neuronal Signaling
Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research
2015-2024
Universidad de Granada
2012
Max Planck Institute for Molecular Biomedicine
2010
Amsterdam UMC Location Vrije Universiteit Amsterdam
2010
Morpho (United States)
2010
Hospital Del Mar
2010
Pyrogenesis (Canada)
2010
Vall d'Hebron Hospital Universitari
2006-2009
Universitat Autònoma de Barcelona
2006-2008
University of California, Los Angeles
2000-2005
Growth of tumors and metastasis are processes known to require neovascularization. To ascertain the participation endogenous angiogenic inhibitor thrombospondin-1 (TSP1) in tumor progression, we generated mammary tumor-prone mice that either lack, or specifically overexpress, TSP1 gland. Tumor burden vasculature were significantly increased TSP1-deficient animals, capillaries within appeared distended sinusoidal. In contrast, overexpressors showed delayed growth lacked frank development (20%...
Mature human aorta contains a 70-kDa versican fragment, which reacts with neoepitope antiserum to the C-terminal peptide sequence DPEAAE. This protein therefore appears represent G1 domain of V1 (G1-DPEAAE441), has been generated in vivo by proteolytic cleavage at Glu441-Ala442 bond, within DPEAAE441-A442RRGQ. Because equivalent aggrecan product (G1-NITEGE341) and brevican (G1-EAVESE395) are ADAMTS-mediated respective proteoglycans, we tested capacity recombinant ADAMTS-1 ADAMTS-4 cleave...
PARP inhibition can induce anti-neoplastic effects when used as monotherapy or in combination with chemo- radiotherapy various tumor settings; however, the basis for anti-metastasic activities resulting from remains unknown. inhibitors may also act modulators of angiogenesis. Proteomic analysis endothelial cells revealed that vimentin, an intermediary filament involved angiogenesis and a specific hallmark EndoMT (endothelial to mesenchymal transition) transformation, was down-regulated...
Understanding tumor induced angiogenesis is a challenging problem with important consequences for diagnosis and treatment of cancer. Recently, strong evidences suggest the dual role endothelial cells on migrating tips proliferating body blood vessels, in consonance further events behind lumen formation vascular patterning. In this paper we present multi-scale phase-field model that combines benefits continuum physics description capability tracking individual cells. The allows us to discuss...
<i>METH-1/ADAMTS1</i> is a member of newly described family genes that contain metalloprotease, disintegrin, and thrombospondin-like motifs. We have recently shown METH-1 protein potent inhibitor angiogenesis. Here, we demonstrate secreted human pro-METH-1 processed in two consecutive steps to release both p87 p65 active forms. The form lacks the N-terminal prodomain results from an additional processing event C-terminal end. Generation was blocked with specific inhibitors furin, incubation...
Metastasis is a sequential process that allows cells to move from the primary tumor and grow elsewhere. Because of their ability cleave variety extracellular signaling adhesion molecules, metalloproteases have been long considered key components metastatic program. However, function certain metalloproteases, such as ADAMTS1, not clear seems depend on cellular environment and/or stage progression. To characterize we performed two alternative proteomic approaches, difference gel...
Sprouting angiogenesis, where new blood vessels grow from pre-existing ones, is a complex process biochemical and mechanical signals regulate endothelial cell proliferation movement. Therefore, mathematical description of sprouting angiogenesis has to take into consideration biological as well relevant physical processes, in particular the interplay between adjacent cells extracellular microenvironment. In this work, we introduce first phase-field continuous model capable predicting sprout...
GLUT1 and GLUT4 glucose transporter expression is highly regulated in muscle adipose tissue during perinatal life. Here we have investigated the role of thyroid hormones regulation induction repression associated to neonatal development. Perinatal hypothyroidism markedly impaired protein heart. This effect was heart specific, a greater detected brown from hypothyroid rats compared with controls. These changes were not or when induced adult rats. results indicate that life sensitive both...
ADAMTS-1 is a metalloprotease that has been implicated in the inhibition of angiogenesis and mediator proteolytic cleavage hyaluronan binding proteoglycans, aggrecan versican. In an attempt to further understand biological function ADAMTS-1, yeast two-hybrid screen was performed using carboxyl-terminal region as bait. As result, extracellular matrix protein fibulin-1 identified potential interacting molecule. Through series analyses included ligand affinity chromatography,...
Progestin-only contraceptives are associated with breakthrough bleeding in up to 50% of users. The causes blood vessel rupture not well understood. Here we report that both normal and Norplant-exposed endothelium express progesterone receptor. Experiments performed vitro on endothelial cells isolated from human endometrium revealed longterm exposure leads suppression cell proliferation, inhibition migration alteration the profile extracellular matrix proteins secreted by endometrial cells....
Proteolytic modification of components the extracellular milieu by metalloproteinases plays important roles in regulation multiple cellular and physiological processes pathological conditions. ADAMTS1 is a secreted enzyme ADAMTS (a disintegrin metalloproteinase with thrombospondin motifs) family proteases, which related to angiogenesis inflammation processes. Here, we describe proteomic screening for putative substrates analyzing protein profiles obtained from cultures transfected cells...
Abstract Mixed-lineage leukemia (MLL)-rearranged (MLLr) infant B-cell acute lymphoblastic (iMLLr-B-ALL) has a dismal prognosis and is associated with pro-B/mixed phenotype, therapy refractoriness frequent central nervous system (CNS) disease/relapse. Neuron-glial antigen 2 (NG2) specifically expressed in MLLr leukemias used immunophenotyping because of its predictive value for leukemias. NG2 involved melanoma metastasis brain development; however, role MLL-mediated leukemogenesis remains...
Natural phenolic compounds have gained momentum for the prevention and treatment of cancer, but their antitumoral mechanism action is not yet well understood. In present study, we screened potential several in a cellular model colorectal cancer (CRC). We selected gallic acid (GA) as candidate terms potency selectivity extensively evaluated its biological activity. report on role GA ligand DNA G-quadruplexes (G4s), explaining effects, including transcriptional inhibition ribosomal CMYC genes....
ADAMTS1 is an extracellular metalloproteinase known to participate in a variety of biological processes that includes inflammation, angiogenesis, and development the urogenital system. Many its functions rely on catalytic activity, which thus far has been limited cleavage matrix proteoglycans aggrecan versican. However, it likely other substrates exist. Using yeast two-hybrid screen, we identified Kunitz-type inhibitor, tissue factor pathway inhibitor-2 (TFPI-2), as binding partner ADAMTS1....
Cancer stem cells have been hypothesized to explain tumor plasticity, including the capability adopt distinct differentiation commitments. Among mechanisms of neovascularization, ability some malignant mimic an endothelial phenotype has recognized by a capacity form matrix-enriched pseudovascular structures. In addition expression genes associated with nature, molecular dynamism specific microenvironments may also be critical. Here, we report identification extracellular protease ADAMTS1 as...
The extracellular protease ADAMTS1 (A disintegrin and metalloprotease with thrombospondin repeats 1) has been described as an anti-angiogenic molecule its role a putative tumor protective also suggested. Here, we have used xenograft model to determine the of in growth angiogenesis. Increasing levels led complete inhibition growth. In attempt elucidate mechanism action this protease, focused our attention on proteolytic activity nidogens, one main components vascular basement membrane....
The clinical management of bone defects caused by trauma or nonunion fractures remains a challenge in orthopedic practice due to the poor integration and biocompatibility properties scaffold implant material. In current work, osteogenic carboxyl-modified single-walled carbon nanotubes (COOH-SWCNTs) were investigated vivo vitro. When human preosteoblasts murine embryonic stem cells cultured on coverslips sprayed with COOH-SWCNTs, accelerated differentiation was manifested increased expression...