A5000204128- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Acute Lymphoblastic Leukemia research
- Diet and metabolism studies
- Ferroptosis and cancer prognosis
- Birth, Development, and Health
- Glioma Diagnosis and Treatment
- Gestational Diabetes Research and Management
- Acute Myeloid Leukemia Research
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Histone Deacetylase Inhibitors Research
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related gene regulation
- Graphene and Nanomaterials Applications
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 detection and testing
- Adipose Tissue and Metabolism
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Biosensors and Analytical Detection
Universidad de Oviedo
2017-2024
Instituto de Investigación Sanitaria del Principado de Asturias
2017-2024
Nanomaterials and Nanotechnology Research Center
2021-2024
Consejo Superior de Investigaciones Científicas
2022-2024
Centre for Biomedical Network Research on Rare Diseases
2021-2024
Instituto de Salud Carlos III
2021-2024
Instituto de Investigación de Enfermedades Raras
2024
Gobierno del Principado de Asturias
2022-2024
Centro de Investigación Biomédica en Red
2021-2023
National Research Council
2023
Summary Cancer is an aging‐associated disease, but the underlying molecular links between these processes are still largely unknown. Gene promoters that become hypermethylated in aging and cancer share a common chromatin signature ES cells. In addition, there also global DNA hypomethylation both processes. However, similarity of regions where this loss methylation occurs currently not well characterized, it unknown if such cancer. To address issue, we analyzed TCGA data from total 2,311...
Abstract Background Obesity is a negative chronic metabolic health condition that represents an additional risk for the development of multiple pathologies. Epidemiological studies have shown how maternal obesity or gestational diabetes mellitus during pregnancy constitute serious factors in relation to appearance cardiometabolic diseases offspring. Furthermore, epigenetic remodelling may help explain molecular mechanisms underlie these epidemiological findings. Thus, this study we explored...
Early life is a period of drastic epigenetic remodeling in which the epigenome especially sensitive to extrinsic and intrinsic influence. However, epigenome-wide dynamics DNA methylation changes that occur during this have not been sufficiently characterized longitudinal studies.To end, we studied status more than 750,000 CpG sites using Illumina MethylationEPIC arrays on 33 paired blood samples from 11 subjects at birth 5 10 years age, then chromatin context associated with these loci by...
Loss of 5-hydroxymethylcytosine (5hmC) has been associated with mutations the ten-eleven translocation (TET) enzymes in several types cancer. However, tumors wild-type TET genes can also display low 5hmC levels, suggesting that other mechanisms involved gene regulation might be implicated decline this epigenetic mark. Here we show DNA hypermethylation and loss hydroxymethylation, as well a marked reduction activating histone marks TET3 gene, impair expression lead to genome-wide levels...
Abstract Type 1 diabetes is a chronic autoimmune disease which results in inefficient regulation of glucose homeostasis and can lead to different vascular comorbidities through life. In this study we aimed analyse the circulating miRNA expression profile patients with type diabetes, no other associated pathology. For this, fasting plasma was obtained from 85 subjects. Next generation sequencing analysis firstly performed identify miRNAs that were differentially expressed between groups (20...
Abstract Aging involves the deterioration of organismal function, leading to emergence multiple pathologies. Environmental stimuli, including lifestyle, can influence trajectory this process and may be used as tools in pursuit healthy aging. To evaluate role epigenetic mechanisms context, we have generated bulk tissue single cell multi-omic maps male mouse dorsal hippocampus young old animals exposed environmental stimulation form enriched environments. We present a molecular atlas aging...
// María G. García 1, 2 , Antonella Carella Rocío Urdinguio Gustavo F. Bayón 1 Virginia Lopez Juan Ramón Tejedor 3 Marta I. Sierra Estela García-Toraño Pablo Santamarina Raúl Perez Cristina Mangas Aurora Astudillo 4 M. Daniela Corte-Torres 5 Inés Sáenz-de-Santa-María 6 María-Dolores Chiara Agustín Fernández and Mario Fraga Institute of Oncology Asturias (IUOPA), HUCA, Universidad de Oviedo,...
Abstract Dementia and cognitive disorders are major aging-associated pathologies. The prevalence severity of these conditions influenced by both genetic environmental factors. Reflecting this, epigenetic alterations have been associated with each processes, especially at the level DNA methylation, such changes may help explain observed interindividual variability in development 2 However, importance explaining their etiology is unclear because little known about timing when they appear....
Aberrant DNA hypermethylation is a hallmark of cancer although the underlying molecular mechanisms are still poorly understood. To study possible role 5-hydroxymethylcytosine (5hmC) in this process we analyzed global and locus-specific genome-wide levels 5hmC 5-methylcytosine (5mC) human primary samples from 12 non-tumoral brains 53 gliomas. We found that identified were significantly reduced Strikingly, hypo-hydroxymethylation at 4627 (9.3%) CpG sites was associated with aberrant strongly...
B cell acute lymphoblastic leukemia (B-ALL) is the most common childhood cancer. As predicted by its prenatal origin, infant B-ALL (iB-ALL) shows an exceptionally silent DNA mutational landscape, suggesting that alternative epigenetic mechanisms may substantially contribute to leukemogenesis. Here, we have integrated genome-wide methylome and transcriptome data from 69 patients with de novo MLL-rearranged (MLLr) non-MLLr iB-ALL uniformly treated according Interfant-99/06 protocol. signatures...
Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, though it known to be essential for embryonic development, its role during adult life still poorly understood. Here we show that this lysine massively hyperacetylated peripheral neutrophils. Genome-wide mapping of H4K16ac terminally differentiated blood cells, along with functional experiments, supported histone post-translational modification the...
To analyze the genome-wide epigenomic and transcriptomic changes induced by long term resistance or endurance training in hippocampus of wild-type mice.We performed whole-genome bisulfite sequencing (WGBS) RNA (RNA-seq) mice after 4 weeks specific training. In addition, we used a novel object recognition test before intervention to determine whether exercise led an improvement cognitive function.Although majority DNA methylation identified this study were training-model specific, most...
Tumor relapse is linked to rapid chemoresistance and represents a bottleneck for cancer therapy success. Engagement of reduced proliferation state non-mutational mechanism exploited by cells bypass therapy-induced cell death. Through combining functional pulse-chase experiments in engineered transcriptomic analyses, we identify DPPA3 as master regulator slow-cycling chemoresistant phenotype colorectal (CRC). We find vicious DPPA3-HIF1α feedback loop that downregulates FOXM1 expression via...
Alternative splicing is a prevalent mechanism of gene regulation that modulated in response to wide range extracellular stimuli. Stress-activated protein kinases (SAPKs) play key role controlling several steps mRNA biogenesis. Here, we show osmostress has an impact on the alternative (AS), which partly mediated through action p38 SAPK. Splicing network analysis revealed functional connection between and spliceosome component SKIIP, whose depletion abolished significant fraction p38-mediated...
Abstract Obesity is associated with adipose tissue dysfunction through the differentiation and expansion of pre-adipocytes to adipocytes (hyperplasia) and/or increases in size pre-existing (hypertrophy). A cascade transcriptional events coordinates into fully differentiated adipocytes; process adipogenesis. Although nicotinamide N-methyltransferase (NNMT) has been obesity, how NNMT regulated during adipogenesis, underlying regulatory mechanisms, remain undefined. In present study we used...
Glioblastoma (GBM) is one of the most aggressive types cancer and exhibits profound genetic epigenetic heterogeneity, making development an effective treatment a major challenge. The recent incorporation molecular features into diagnosis patients with GBM has led to improved categorization various tumour subtypes different prognoses disease management. In this work, we have exploited benefits genome-wide multi-omic approaches identify potential vulnerabilities existing in GBM. Integration...
Promoter hypermethylation of tumour suppressor genes is frequently observed during the malignant transformation colorectal cancer (CRC). However, whether this epigenetic mechanism functional in or a mere consequence carcinogenic process remains to be elucidated.In work, we performed an integrative multi-omic approach identify gene candidates with strong correlations between DNA methylation and expression human CRC samples set 8 colon cell lines. As proof concept, combined recent CRISPR-Cas9...
The OLR1 gene encodes the oxidized low-density lipoprotein receptor (LOX-1), which is responsible for cellular uptake of LDL (Ox-LDL), foam cell formation in atheroma plaques and atherosclerotic plaque rupture. Alternative splicing (AS) exon 5 generates two protein isoforms with antagonistic functions Ox-LDL uptake. Previous work identified six single nucleotide polymorphisms (SNPs) linkage disequilibrium that influence inclusion levels correlate risk cardiovascular disease. Here we use...