A5048986415- Analytical chemistry methods development
- Environmental Toxicology and Ecotoxicology
- Marine Biology and Environmental Chemistry
- Mercury impact and mitigation studies
- Mass Spectrometry Techniques and Applications
- Isotope Analysis in Ecology
- Toxic Organic Pollutants Impact
- Metabolomics and Mass Spectrometry Studies
- Marine Ecology and Invasive Species
- Pharmacological Effects and Assays
- Analytical Chemistry and Chromatography
- Environmental Chemistry and Analysis
- Heavy Metal Exposure and Toxicity
- Hormonal and reproductive studies
- Metabolism and Genetic Disorders
- Chromium effects and bioremediation
- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Pesticide Residue Analysis and Safety
- Heavy metals in environment
- Air Quality and Health Impacts
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Radioactive element chemistry and processing
Universidad de Oviedo
2015-2024
Consejo de Ciencia y Tecnología del Estado de Tabasco
2023
Universidad de León
2023
Instituto de Investigación Sanitaria del Principado de Asturias
2021
Instituto de Investigación Biosanitaria de Granada
2021
Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux
2007-2018
Centre National de la Recherche Scientifique
2006-2018
Faculty (United Kingdom)
2015
Université de Pau et des Pays de l'Adour
2006-2014
Universitat Jaume I
2010
This work reports the first results on stable isotope fractionation of Hg during methylation by anaerobic bacteria under dark conditions. The GC-MC-ICPMS methodology employed is capable simultaneously measuring species-specific isotopic composition different species within same sample. We have studied caused Hg(II) standard reference material NIST-3133 in presence pure bacterial strain Desulfobulbus propionicus MUD10 (DSM 6523) fermentative measured and monomethyl mercury (MMHg) these...
Microplastics are one of the major pollutants in aquatic environments. Among their components, Bisphenol A (BPA) is most abundant and dangerous, leading to endocrine disorders deriving even different types cancer mammals. However, despite this evidence, xenobiotic effects BPA over plantae microalgae still need be better understood at molecular level. To fill gap, we characterized physiological proteomic response Chlamydomonas reinhardtii during long-term exposure by analyzing biochemical...
Abstract The use of species‐specific isotopic tracers for inorganic and methyl mercury has allowed the simultaneous determination methylation demethylation potentials pure culture isolated sulfate‐reducing (SR) bacterial strains using low Hg species concentration levels (7 µg/L 199 Hg(II), 1 Me 201 Hg). A major advantage method reported here is that it can be used to follow simultaneously both degradation added but also formation their products thus during same incubation specific...
This work presents the simultaneous online determination of isotopic composition different Hg species in a single sample by hyphenation gas chromatography (GC) with multicollector-inductively coupled plasma mass spectrometry (MC-ICPMS). With use commercially available instrumentation, precise and accurate species-specific isotope δ values (per mil deviation ratio relative to reference standard) have been obtained from consecutive GC transient signals. The isothermal temperature programs...
This work describes the first multiple spiking isotope dilution procedure for organic compounds using 13C labeling. A double-spiking method capable of correcting and quantifying creatine–creatinine interconversion occurring during analytical determination both in human serum is presented. The creatinine may be affected by between creatine sample preparation or inefficient chemical separation those solid phase extraction (SPE). methodology based on use differently labeled analogues...
The pineal neuroindole melatonin exerts an exceptional variety of systemic functions. Some them are exerted through its specific membrane receptors type 1 and 2 (MT1 MT2) while others mediated by receptor-independent mechanisms. A potential transport facilitative glucose transporters (GLUT/SLC2A) was proposed in prostate cancer cells. cells have a particular metabolism that changes during tumor progression. During the first steps carcinogenesis, oxidative phosphorylation is reactivated...
With the aim to distinguish between routes of exposition mercury (Hg) in artisanal and small-scale gold mining (ASGM) communities Hg contamination sources, species composition should be performed human biomarkers. In this work, species-specific determination were determined hair samples (N = 96), mostly non-directly occupied ASGM tasks, from six most relevant Colombian regions. Therefore, MeHg, Hg(II) THg concentrations simultaneously by double spiking isotope dilution mass spectrometry...
The application range of high-precision isotopic analysis for identifying the origin Hg in environmental compartments was extended to samples with lower concentrations levels by using faraday cup amplifiers higher ohmic resistance.
Different liquid−solid extraction techniques, including room-temperature leaching with mechanical shaking, ultrasonic, and microwave-assisted extractions, have been evaluated for the quantitative speciation of tin mono-, di-, tributyltin (MBT, DBT, TBT, respectively) in PACS-2 BCR-646 certified reference materials. A methanol−acetic acid mixture was used as extractant reagent all cases. For this purpose, a mixed spike containing 119Sn-enriched MBT (79.7 At%), 118Sn-enriched DBT (86.7 TBT...
Following the concepts and applications included in our tutorial review published 2005, this presents critically discusses new concepts, methodologies trends that have appeared during last five years of scientific development field isotope dilution analysis for elemental speciation. Although general been detailed previously several high quality reviews, we will highlight selected that, opinion, significantly contributed to improvement state art field. Methodological advances, such as...
This work demonstrates, for the first time, applicability of multiple spiking isotope dilution analysis to molecular mass spectrometry exemplified by speciation mercury using GC(EI)MS instrumentation. A double spike approach isotopically enriched isotopes has been applied determination inorganic Hg(II) and methylmercury (MeHg) in fish reference materials. The method is based on application pattern deconvolution simultaneous degradation-corrected concentrations mercury. Mass isotopomer...
We present here a new environmental measurement method for the rapid extraction and accurate quantification of Cr(VI) in solid samples. The quantitative is achieved 10 minutes by means focused microwave assisted using 50 mmol/L Ethylendiamintetraacetic acid (EDTA) at pH as extractant. In addition, it enables separation Cr species anion exchange chromatography mobile phase which 1:10 dilution extracting solution. Thus, neutralization or acidification steps are prone to cause interconversion...
We report the first common methodology for simultaneous determination of methylmercury (MeHg), ethylmercury (EtHg), and inorganic mercury (Hg(II)) in human blood hair urine. With exception initial sample mass (0.15 g blood, 0.5 urine, 0.1 hair), same preparation gas chromatography-inductively coupled plasma spectrometry (GC-ICPMS) measurement conditions are employed three matrixes providing experimental values agreement with certified analysis NIST SRM 955c (Caprine Blood) Level 3 hairs IAEA...
An examination and comparison of all the mathematical approaches for multiple spiking species-specific isotope dilution analysis published so far in literature is presented this work with determination TBT DBT sediments. The basis four different numerical approaches—Calculation Stable Isotope Concentrations, Speciated Dilution Analysis, Species-Specific Analysis Pattern Deconvolution—are explained compared terms complexity, analytical figures merit specific advantages. methodologies have...
A procedure for Stable Isotope Dilution Analysis in molecular Mass Spectrometry which does not require a methodological calibration graph and can be applied combination with minimal labelling has been evaluated. This alternative approach is based on the determination of molar fractions each pure isotope pattern (natural abundance or labelled) contributing to observed mixture natural labelled molecules by multiple linear regression. Two compounds, (13)C(1)-labelled (13)C(6)-labelled phenol,...