A5005617864- Pluripotent Stem Cells Research
- Acute Lymphoblastic Leukemia research
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Hematopoietic Stem Cell Transplantation
- Epigenetics and DNA Methylation
- Chronic Lymphocytic Leukemia Research
- Mesenchymal stem cell research
- Zebrafish Biomedical Research Applications
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Lymphoma Diagnosis and Treatment
- Renal and related cancers
- Chronic Myeloid Leukemia Treatments
- T-cell and B-cell Immunology
- Sarcoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Protein Degradation and Inhibitors
- RNA modifications and cancer
Josep Carreras Leukaemia Research Institute
2016-2025
Universitat de Barcelona
2016-2025
Instituto de Salud Carlos III
2010-2025
Institució Catalana de Recerca i Estudis Avançats
2016-2025
Centro de Investigación Biomédica en Red
2008-2025
Centro de Investigación Biomédica en Red de Cáncer
2017-2025
Fundación Josep Carreras Contra la Leucemia
2025
Hospital Sant Joan de Déu Barcelona
2024-2025
Spanish Clinical Research Network
2020-2024
Heinrich Heine University Düsseldorf
2024
Chromosomal rearrangements of the human MLL/KMT2A gene are associated with infant, pediatric, adult and therapy-induced acute leukemias. Here we present data obtained from 2345 leukemia patients. Genomic breakpoints within MLL involved translocation partner genes (TPGs) were determined 11 novel TPGs identified. Thus, a total 135 different have been identified so far, which 94 now characterized at molecular level. In all, 35 out these occur recurrently, but only 9 specific fusions account for...
Chromosomal rearrangements of the human MLL (mixed lineage leukemia) gene are associated with high-risk infant, pediatric, adult and therapy-induced acute leukemias. We used long-distance inverse-polymerase chain reaction to characterize chromosomal rearrangement individual leukemia patients. present data molecular characterization 1590 MLL-rearranged biopsy samples obtained from The precise localization genomic breakpoints within involved translocation partner genes (TPGs) were determined...
Abstract Chromosomal rearrangements of the human KMT2A/MLL gene are associated with de novo as well therapy-induced infant, pediatric, and adult acute leukemias. Here, we present data obtained from 3401 leukemia patients that have been analyzed between 2003 2022. Genomic breakpoints within KMT2A involved translocation partner genes (TPGs) -partial tandem duplications (PTDs) were determined. Including published literature, a total 107 in-frame fusions identified so far. Further 16...
A hallmark of cancer cells is their remarkable ability to efficiently adapt favorable and hostile environments. Due a unique metabolic flexibility, tumor can grow even in the absence extracellular nutrients or stressful scenarios. To achieve this, need large amounts lipids build membranes, synthesize lipid‐derived molecules, generate energy other nutrients. Tumor potentiate strategies obtain from cells, pathways new lipids, mechanisms for efficient storage, mobilization, utilization these...
Despite the need for alternative sources of human hematopoietic stem cells (HSCs), functional capacity generated from embryonic (hESCs) has yet to be evaluated and compared with adult sources. Here, we report that somatic hESC-derived have similar phenotype in vitro clonogenic progenitor activity. However, contrast cells, failed reconstitute intravenously transplanted recipient mice because cellular aggregation causing fatal emboli formation. Direct femoral injection allowed survival...
MicroRNAs (miRNAs) play a central role in the regulation of multiple biological processes including maintenance stem cell self-renewal and pluripotency. Recently, miRNA cluster miR302-367 was shown to be differentially expressed embryonic cells (ESCs). Unfortunately, very little is known about genomic structure miRNA-encoding genes their transcriptional units. Here, we have characterized gene coding for human cluster. We identify start functional core promoter region which specifically...
Abstract Background The use of human embryonic stem cells (hESCs) in research is increasing and hESCs hold the promise for many biological, clinical toxicological studies. Human ESCs are expected to be chromosomally stable since karyotypic changes represent a pitfall potential future applications. Recently, several studies have analysed genomic stability hESC lines maintained after prolonged vitro culture but controversial data has been reported. Here, we prompted compare chromosomal three...
Half the human genome is made of transposable elements (TEs), whose ongoing activity continues to impact our genome. LINE-1 (or L1) an autonomous non-LTR retrotransposon in genome, comprising 17% its genomic mass and containing average 80-100 active L1s per that provide a source inter-individual variation. New insertions are thought accumulate mostly during embryogenesis. Surprisingly, can further somatic brain However, it currently unknown whether L1 retrotranspose other healthy tissues or...
Human ESCs provide access to the earliest stages of human development and may serve as an unlimited source functional cells for future cell therapies. The optimization methods directing differentiation embryonic stem (hESCs) into tissue-specific precursors becomes crucial. We report efficient enrichment mesenchymal (MSCs) from hESCs through specific inhibition SMAD-2/3 signaling. ESC-derived MSCs (hESC-MSCs) emerged a population fibroblastoid expressing MSC phenotype: CD73+ CD90+ CD105+...
In differentiated cells, aging is associated with hypermethylation of DNA regions enriched in repressive histone post-translational modifications. However, the chromatin marks changes methylation adult stem cells during lifetime are still largely unknown. Here, profiling mesenchymal (MSCs) obtained from individuals aged 2 to 92 yr identified 18,735 hypermethylated and 45,407 hypomethylated CpG sites aging. As sequences were marks. Most importantly, strongly active mark H3K4me1 suggesting...
Genetically modifying autologous T cells to express an anti-CD19 chimeric antigen receptor (CAR) has shown impressive response rates for the treatment of CD19+ B cell malignancies in several clinical trials (CTs). Making this available our patients prompted us develop a novel CART19 based on own antibody (A3B1), followed by CD8 hinge and transmembrane region, 4-1BB- CD3z-signaling domains. We show that A3B1 CAR are highly cytotoxic specific against vitro, inducing secretion pro-inflammatory...