A5091466118- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- Virus-based gene therapy research
- Ocular Oncology and Treatments
- Tissue Engineering and Regenerative Medicine
- Acute Myeloid Leukemia Research
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Biomedical Ethics and Regulation
- Bacteriophages and microbial interactions
- Chromosomal and Genetic Variations
- Microbial infections and disease research
- Sarcoma Diagnosis and Treatment
- Mesenchymal stem cell research
- Acute Lymphoblastic Leukemia research
- Molecular Biology Techniques and Applications
- Aquaculture disease management and microbiota
- Multiple Myeloma Research and Treatments
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Telomeres, Telomerase, and Senescence
- Prion Diseases and Protein Misfolding
Andalusian School of Public Health
2012-2024
Universidad de Granada
2008-2018
Ministry of Health
2018
Parque Tecnológico de la Salud
2007-2010
Instituto de Salud Carlos III
2010
Centro Nacional de Microbiologia
2010
Complejo Hospitalario de Pontevedra
2008
Hospital Universitario Virgen de las Nieves
2005-2007
Abstract Background The use of human embryonic stem cells (hESCs) in research is increasing and hESCs hold the promise for many biological, clinical toxicological studies. Human ESCs are expected to be chromosomally stable since karyotypic changes represent a pitfall potential future applications. Recently, several studies have analysed genomic stability hESC lines maintained after prolonged vitro culture but controversial data has been reported. Here, we prompted compare chromosomal three...
MLL-AF4 fusion is a hallmark genetic abnormality in infant B-acute lymphoblastic leukemia (B-ALL) known to arise utero. The cellular origin of leukemic genes during human development difficult ascertain. bone marrow (BM) microenvironment plays an important role the pathogenesis several hematological malignances. BM mesenchymal stem cells (BM-MSC) from 38 children diagnosed with cytogenetically different acute leukemias were screened for genes. Fusion absent BM-MSCs childhood carrying...
There is growing evidence about the role of mesenchymal stem cells (MSCs) as cancer in many sarcomas. Nevertheless, little still known cellular and molecular mechanisms underlying MSCs transformation. We aimed at investigating p53 p21, two important regulators cell cycle progression apoptosis normally involved protection against tumorigenesis. Mesenchymal from wild-type, p21-/-p53+/+, p21-/-p53+/- mice were cultured vitro analyzed for appearance tumoral transformation properties after low,...
Sarcomas have been modeled in mice by the expression of specific fusion genes mesenchymal stem cells (MSC), supporting concept that MSCs might be target initiating cell sarcoma. In this study, we evaluated potential oncogenic effects p53 and/or retinoblastoma (Rb) deficiency MSC transformation and sarcomagenesis. We derived wild-type, p53(-/-), Rb(-/-), p53(-/-)Rb(-/-) cultures fully characterized their vitro growth properties vivo tumorigenesis capabilities. contrast with wild-type MSCs,...
MLL rearrangements are hallmark genetic abnormalities in infant leukemia known to arise utero . They can be induced during human prenatal development upon exposure etoposide. We also hypothesize that chronic etoposide might render cells more susceptible other genomic insults. Here, for the first time, embryonic stem (hESCs) were used as a model test effects of on early development. addressed whether: (i) low doses promote hESCs and hESCs-derived hematopoietic cells; (ii) sufficient confer...
Objective Toxic thyroid adenoma (TA) is a common cause of hyperthyroidism. Mutations in the TSH receptor ( TSHR ) gene, and less frequently adenylate cyclase-stimulating G alpha protein GNAS are well established causes TA Europe. However, genetic remain unknown small percentage cases. We report first study to investigate mutations , kinase, cAMP-dependent, regulatory, type I PRKAR1A RAS genes, large series from Galicia, an iodine-deficient region NW Spain. Design methods Eighty-five samples...
Establishment of continuous cell lines from human normal and tumor tissues is an extended useful methodology for molecular characterization cancer pathophysiology drug development in research laboratories. The exchange these between different labs a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells flasks experiment reproducibility reliability. Great proportions are contaminated and/or replaced by derived origin during...
BACKGROUNDHuman embryonic stem cells (hESCs) have opened up a new area of research in biomedicine. The efficiency hESC derivation from frozen poor-quality embryos is low and normally achieved by plating on mouse or human foreskin feeders (HFFs). We attempted to optimize embryo survival derivation.
Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) as feeders for embryonic stem cells (hESC). We tested ability stop HFF and induce apoptosis. inhibited at 10 microg/ml over 2.5h showing a decrease in index measured by Ki-67 S G2/M phases related active HFF. A low percentage showed necrotic or apoptotic features using different lengths incubation. Over time, majority remained mitotically...
The optimization of human embryonic stem (hES) cell line derivation methods is challenging because many worldwide laboratories have neither access to spare embryos nor ethical approval for using supernumerary hES purposes. Additionally, studies performed directly on imply a waste precious biological material. In this study, we developed new strategy based the combination whole-blastocyst culture followed by laser drilling destruction trophoectoderm improving efficiency inner mass (ICM)...
Human embryonic stem cells (hESCs) represent a promise for future strategies of tissue replacement. However, there are different issues that should be resolved before these can used in cellular therapies; among others, the rejection transplantable hESCs as result HLA incompatibility between donor and recipients. The exhibit weak class I expression on cell surface, but today responsible mechanisms unknown. We have analyzed level heavy chain, beta2-microglobulin (beta2-m), antigen-processing...
Abstract Pediatric Acute Myeloid Leukemia (AML) is a rare and heterogeneous disease characterized by high prevalence of gene fusions as driver mutations. Despite the improvement survival in last years, about 50% patients still experience relapse. It not possible to improve prognosis only with further intensification chemotherapy, come severe cost health patients, often resulting treatment-related death or long-term sequels. To design more effective less toxic therapies we need better...
Abstract To further contribute to the understanding of multiple myeloma, we have focused our research interests on mechanisms by which tumour plasma cells a higher survival rate than normal cells. In this article, study expression profile genes involved in regulation and protection telomere length, telomerase activity apoptosis samples from patients with monoclonal gammopathy undetermined significance, smouldering myeloma ( MM ) cell leukaemia PCL ), as well several human lines HMCL s)....
ABSTRACT Alu elements are non-autonomous Short INterspersed Elements (SINEs) derived from the 7SL RNA gene that present at over one million copies in human genomic DNA. mobilizes by a mechanism known as retrotransposition, which requires Long Element-1 (LINE-1 or L1) ORF2 -encoded protein (ORF2p). Here, we demonstrate HeLa strains differ their capacity to support retrotransposition. Human retrotranspose efficiently HeLa-HA and HeLa-CCL2 ( -permissive) strains, but not HeLa-JVM HeLa-H1...
Abstract Alu elements are non-autonomous Short INterspersed Elements (SINEs) derived from the 7SL RNA gene that present at over one million copies in human genomic DNA. mobilizes by a mechanism known as retrotransposition, which requires Long Element-1 (LINE-1) ORF2-encoded protein (ORF2p). Here, we demonstrate HeLa strains differ their capacity to support retrotransposition. Human retrotranspose efficiently HeLa-HA and HeLa-CCL2 (Alu-permissive) strains, but not HeLa-JVM or HeLa-H1...
Biobanks are infrastructures essential for research involving multi-disciplinary teams and an increasing number of stakeholders. In the field personalized medicine, biobanks play a key role through provision well-characterized annotated samples protecting at same time right donors. The Andalusian Public Health System Biobank (SSPA Biobank) has implemented global information management system made up different modules that allow recording, traceability monitoring all associated with biobank...
Peripheral blood is the most promising source of RNA biomarkers for diagnostic and epidemiological studies, because presence disease prognostic information reflected in gene expression pattern. Quality used by a number different downstream applications, so selection appropriate stabilization purification method important. We have analyzed purified from 300 samples 25 donors processed two technicians using three methodologies with Tempus PaxGene tubes.The best quality sample results were...