A5048014143- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- 3D Printing in Biomedical Research
- CAR-T cell therapy research
- melanin and skin pigmentation
- Genomics, phytochemicals, and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Peptidase Inhibition and Analysis
- Ultrasound and Hyperthermia Applications
- Cancer Genomics and Diagnostics
- Circular RNAs in diseases
- Receptor Mechanisms and Signaling
- Photochromic and Fluorescence Chemistry
- Renal and related cancers
- FOXO transcription factor regulation
Universidad de Granada
2016-2025
Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research
2021-2025
Instituto de Investigación Biosanitaria de Granada
2016-2025
ADA University
2024
Regenerative Medicine Institute
2024
Complejo Hospitalario de Jaén
2022-2023
Abstract Cancer stem cells are responsible for tumor progression, metastasis, therapy resistance and cancer recurrence, doing their identification isolation of special relevance. Here we show that low adherent breast colon subpopulations have stem-like properties. Our results demonstrate trypsin-sensitive (TS) increased ALDH activity, higher ability to exclude Hoechst 33342, enlarged proportion with a cell phenotype enriched in sphere- colony-forming vitro . Further studies MDA-MB-231 reveal...
The use of decellularized extracellular matrix (dECM) as a biomaterial has been an important step forward for the development functional tissue constructs. In addition to tissues and organs, cell cultures are gaining lot attention alternative source dECM. this work, novel biomimetic hydrogel is developed based on dECM obtained from mesenchymal stem cells (mdECM) cartilage engineering. To end, seeded under specific culture conditions generate early chondrogenic (ECM) providing cues elements...
Abstract Background Active targeting by surface-modified nanoplatforms enables a more precise and elevated accumulation of nanoparticles within the tumor, thereby enhancing drug delivery efficacy for successful cancer treatment. However, surface functionalization involves complex procedures that increase costs timelines, presenting challenges clinical implementation. Biomimetic (BNPs) have emerged as unique platforms overcome limitations actively targeted nanoparticles. Nevertheless, BNPs...
Pancreatic ductal adenocarcinoma is the third leading cause of cancer-related death. Its dismal prognosis closely related to late diagnosis, which results from both non-specific symptoms and absence biomarkers for early diagnosis. MicroRNAs (miRNAs) exert a regulatory role in numerous biological processes their aberrant expression has been found broad spectrum diseases, including cancer. Cancer stem cells (CSCs) represent driving force PDAC initiation, progression meta-static spread. Our...
Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related death. Its poor prognosis closely related to late-stage diagnosis, which results from both nonspecific symptoms and absence biomarkers for early diagnosis. MicroRNAs (miRNAs) exert a regulatory role in numerous biological processes their aberrant expression has been found broad spectrum diseases, including cancer. Cancer stem cells (CSCs) represent driving force PDAC initiation, progression, metastatic...
Biofabrication technologies with layer-by-layer simultaneous deposition of a polymeric matrix and cell-laden bioinks (also known as bioprinting) offer an alternative to conventional treatments regenerate cartilage tissue. Thermoplastic polymers, like poly-lactic acid, are easy print using fused modeling, the shape, mesh structure, biodegradation time, stiffness can be easily controlled. Besides some them being clinically approved, high manufacturing temperatures used in bioprinting...
In breast cancer (BC), the presence of stem cells (CSCs) has been related to relapse, metastasis, and radioresistance. Radiotherapy (RT) is an extended BC treatment, but not always effective. CSCs have several mechanisms radioresistance in place, some miRNAs are involved cellular response ionizing radiation (IR). Here, we studied how IR affects expression stemness different molecular subtypes. Exposition doses 2, 4, or 6 Gy affected their phenotype, functional characteristics, pluripotency...
The latest trends in cancer research and nanomedicine focus on using nanocarriers to target stem cells (CSCs). Specifically, lipid liquid nanocapsules are usually developed as for lipophilic drug delivery. Here, we olive oil NCs (O2LNCs) functionalized by covalent coupling of an anti-CD44-fluorescein isothiocyanate antibody (αCD44). First, O2LNCs formed a core surrounded shell containing phospholipids, nonionic surfactant, deoxycholic acid molecules. Then, were coated with αCD44...
Colorectal cancer (CRC) is one of most common cancers worldwide, with high rates mortality. Epidemiological findings demonstrate that coffee consumption reduces the risk developing CRC by ~13%. In general, in vivo and vitro antiproliferative, antioxidant proapoptotic effects brewed or major bioavailable compounds. Thus, it was assessed whether caffeine (CAF) and/or chlorogenic acid (CGA) attenuates early-stage chemically induced mouse colon carcinogenesis. Male Swiss mice were submitted to a...
Triple-negative breast cancer (TNBC) is characterised by its aggressiveness and resistance to chemotherapy, demanding the development of effective strategies against unique characteristics. Derived from lapacho tree bark, β-lapachone (β-LP) selectively targets cells with elevated levels detoxifying enzyme NQO1. Hydroxytyrosol (HT) a phenolic compound derived olive trees important anticancer properties that include inhibition stem (CSCs) metastatic features in TNBC, as well relevant...
Colorectal cancer (CRC) is a deadly tumour in Western countries characterized by high cellular/molecular heterogeneity. Cancer stem cells (CSC) act recurrence, drug-resistance and metastatic epithelial-to-mesenchymal transition. microRNAs (miRNAs) contribute to increasing, miRNA roles CSC phenotype fate their utility as CRC biomarkers have also been reported. Here, we investigated miR-21, miR-221, miR-18a, miR-210, miR-31, miR-34a, miR-10b miR-16 expression experimental ALDH+ CD44+/CD326+...
DPP IV, otherwise known as CD26 lymphocyte T surface antigen, is a transmembrane glycoprotein also found in circulation the blood. It plays an important role several processes like glucose metabolism and T-cell stimulation. Moreover, it overexpressed renal, colon, prostate, thyroid human carcinoma tissues. can serve diagnostic patients with lysosomal storage diseases. The biological clinical importance of having readouts for activity this enzyme, physiological disease conditions, has led us...
Colorectal cancer (CRC) is the third most frequent worldwide and second cause of deaths. Increasing evidences supports idea that poor prognosis patients related to presence stem cells (CSCs), a cell population able drive recurrence metastasis. The deregulation microRNAs (miRNAs) plays role in formation CSC. We investigated hsa-miR-486-5p (miR-486-5p) CRC, CSCs, metastasis, order reach better understanding biomolecular epigenetic mechanisms mir-486-5p-related. expression miR-486-5p was three...
Abstract Oncogenic microRNAs (miRs) have emerged as diagnostic biomarkers and novel molecular targets for anti-cancer drug therapies. Real-time quantitative PCR (qPCR) is one of the most powerful techniques analyzing miRs; however, use unsuitable normalizers might bias results. Tumour heterogeneity makes even more difficult selection an adequate endogenous normalizer control. Here, we evaluated five potential referenced small RNAs ( U6, rRNA5s, SNORD44, SNORD24 hsa-miR-24c-3p ) using...
Despite the great advances in cancer treatment, colorectal has emerged as second highest cause of death from worldwide. For this type tumor, use suicide gene therapy could represent a novel therapy. We recently demonstrated that co-expression gef and apoptin dramatically inhibits proliferation DLD-1 colon cell line. In present manuscript, we try to establish mechanism underlying enhanced induction apoptosis by triggering both expression tumor cells. Scanning microscopy reveals simultaneous...
Abstract Breast cancer (BC) is the most common tumour in women and one of important causes death worldwide. Radiation therapy (RT) widely used for BC treatment. Some proteins have been identified as prognostic factors (Ki67, p53, E‐cadherin, HER2). In last years, it has shown that variations expression MMPs TIMPs may contribute to development BC. The aim this pilot work was study effects RT on different (‐1, ‐2, ‐3, ‐7, ‐8, ‐9, ‐10, ‐12 ‐13) (‐1 ‐4), well their relationship with other...