A5082845707- 3D Printing in Biomedical Research
- Osteoarthritis Treatment and Mechanisms
- Additive Manufacturing and 3D Printing Technologies
- Bone Tissue Engineering Materials
- Wound Healing and Treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- Periodontal Regeneration and Treatments
- Collagen: Extraction and Characterization
- Electrospun Nanofibers in Biomedical Applications
- Lower Extremity Biomechanics and Pathologies
- Cancer Cells and Metastasis
- Knee injuries and reconstruction techniques
- Tissue Engineering and Regenerative Medicine
- Pregnancy and preeclampsia studies
- Mesenchymal stem cell research
- Surgical Sutures and Adhesives
- Birth, Development, and Health
- Cellular Mechanics and Interactions
- Cardiovascular Disease and Adiposity
- Photoacoustic and Ultrasonic Imaging
- Advanced Theoretical and Applied Studies in Material Sciences and Geometry
- Hydrogels: synthesis, properties, applications
- Aortic Disease and Treatment Approaches
- Thermoregulation and physiological responses
- Tendon Structure and Treatment
Universidad de Granada
2016-2025
Instituto de Investigación Biosanitaria de Granada
2015-2025
National Center for Advancing Translational Sciences
2024-2025
National Institutes of Health
2022
Regenerative Medicine Institute
2019
Bioprinting is a promising tool to fabricate well-organized cell-laden constructs for repair and regeneration of articular cartilage. The selection suitable bioink, in terms composition mechanical properties, crucial the development viable cartilage substitutes. In this study, we focused on use one main components, hyaluronic acid (HA), design formulate new bioink tissue 3D bioprinting. Major characteristics required application such as printability, biocompatibility, biodegradability were...
The use of decellularized extracellular matrix (dECM) as a biomaterial has been an important step forward for the development functional tissue constructs. In addition to tissues and organs, cell cultures are gaining lot attention alternative source dECM. this work, novel biomimetic hydrogel is developed based on dECM obtained from mesenchymal stem cells (mdECM) cartilage engineering. To end, seeded under specific culture conditions generate early chondrogenic (ECM) providing cues elements...
Biofabrication technologies with layer-by-layer simultaneous deposition of a polymeric matrix and cell-laden bioinks (also known as bioprinting) offer an alternative to conventional treatments regenerate cartilage tissue. Thermoplastic polymers, like poly-lactic acid, are easy print using fused modeling, the shape, mesh structure, biodegradation time, stiffness can be easily controlled. Besides some them being clinically approved, high manufacturing temperatures used in bioprinting...
Abstract Conventional in vitro cancer models do not accurately reproduce the tumor microenvironment (TME), so three-dimensional (3D)-bioprinting represents an excellent tool to overcome their limitations. Here, two multicellular tri-layered malignant melanoma (MM) composed by stem cells (CSCs) isolated from a MM established cell line or primary-patient derived line, fibroblasts, mesenchymal cells, and endothelial embedded within agarose-collagen type I hydrogel were bioprinted....
Wound healing is a natural physiological reaction to tissue injury. Hydrogels show attractive advantages in wound not only due their biodegradability, biocompatibility and permeability but also because provide an excellent environment for cell migration proliferation. The main objective of the present study was design characterization hydrogel loaded with human mesenchymal stromal cells (hMSCs) use would superficial skin injures. Poloxamer 407® used as biocompatible biomaterial embed hMSCs....
Abstract Three-dimensional (3D) bioprinting is considered one of the most advanced tools to build up materials for tissue engineering. The aim this work was design, development and characterization a bioink composed human mesenchymal stromal cells (hMSC) extrusion through nozzles create these 3D structures that might potentially be apply replace function damaged natural tissue. In study, we focused on advantages wide potential biocompatible biomaterials, such as hyaluronic acid alginate...
Abstract Tissue engineering (TE) seeks to fabricate implants that mimic the mechanical strength, structure, and composition of native tissues. Cartilage TE requires development functional personalized with cartilage‐like properties capable sustaining high load‐bearing environments integrate into surrounding tissue cartilage defect. In this study, we evaluated novel 1,4‐butanediol thermoplastic polyurethane elastomer (b‐TPUe) derivative filament as a 3D bioprinting material application in TE....
Research on the biology of fetal-maternal barriers has been limited by access to physiologically relevant cells, including trophoblast cells. In this study, we describe development a human term placenta-derived cytotrophoblast immortalized cell line (hPTCCTB) derived from basal plate. Human-term cells are comparable their primary origin in terms morphology, marker expression, and functional responses. We demonstrate that these can transform into syncytiotrophoblast extravillous trophoblasts....
Due to the limitations of current skin wound treatments, it is highly valuable have a healing formulation that mimics extracellular matrix (ECM) and mechanical properties natural tissue. Here, novel biomimetic hydrogel has been developed based on mixture Agarose-Collagen Type I (AC) combined with ECM-related components: Dermatan sulfate (DS), Hyaluronic acid (HA), Elastin (EL) for its application in tissue engineering (TE). Different formulations were designed by combining AC hydrogels DS,...
Three-dimensional bioprinting technology has enabled great advances in the treatment of articular cartilage (AC) defects by biofabrication biomimetic constructs that restore and/or regenerate damaged tissue. In this sense, selection suitable cells and biomaterials to bioprint mimic architecture, composition, functionality natural extracellular matrix (ECM) native tissue is crucial. present study, a novel cartilage-like hybrid construct (CBC) was developed 3D facilitate promote AC regeneration.
Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks, and hazards thrombus formation still need to be addressed. In this study, we assess mechanical properties two groups porcine carotid blood vessels: (i) native arteries (ii) arteries. The both (n = 10, sample size each group) determined by conducting uniaxial circumferential tensile...
Engineered cartilage tissue is one of the most promising treatments for articular pathologies. In this work, an ultrasonic bioreactor was designed to implement a non-invasive real-time monitoring neo-cartilage formation processes through signal analysis. Polylactic acid (PLA) scaffolds were printed and seeded with human chondrocytes. Then, they cultured in ultrasound (US)-integrated bioreactor. The readings from sensors analyzed by numerical models ultrasound-tissue interaction stochastic...
Due to the limitations of current skin wound treatments, it is highly valuable have a healing formulation that mimics extracellular matrix (ECM) and mechanical properties natural tissue. Here, novel biomimetic hydrogel has been developed based on an Agarose-Collagen Type I (AC) blend combined with ECMrelated components: Dermatan sulphate (DS), Hyaluronic acid (HA), Elastin (EL) for its application in tissue engineering (TE). Different formulations were elaborated AC DS, HA EL. Cell...