A5024706175- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Virus-based gene therapy research
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- Psoriasis: Treatment and Pathogenesis
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Cutaneous Melanoma Detection and Management
- Anatomy and Medical Technology
- Osteoarthritis Treatment and Mechanisms
- Cancer Research and Treatments
- Melanoma and MAPK Pathways
- Periodontal Regeneration and Treatments
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Medical and Biological Sciences
- Electrospun Nanofibers in Biomedical Applications
- Innovations in Medical Education
- Dermatology and Skin Diseases
- melanin and skin pigmentation
- Cancer therapeutics and mechanisms
- Cancer Treatment and Pharmacology
- Cancer Cells and Metastasis
Universidad de Granada
2015-2025
Instituto de Investigación Biosanitaria de Granada
2009-2025
ADA University
2022-2023
Charles R. Drew University of Medicine and Science
2020
Southwest Texas Junior College
2014
Weatherford College
2012
Regenerative Medicine Institute
2006-2011
Universidad de Jaén
2002-2004
Bioprinting is a promising tool to fabricate well-organized cell-laden constructs for repair and regeneration of articular cartilage. The selection suitable bioink, in terms composition mechanical properties, crucial the development viable cartilage substitutes. In this study, we focused on use one main components, hyaluronic acid (HA), design formulate new bioink tissue 3D bioprinting. Major characteristics required application such as printability, biocompatibility, biodegradability were...
The use of decellularized extracellular matrix (dECM) as a biomaterial has been an important step forward for the development functional tissue constructs. In addition to tissues and organs, cell cultures are gaining lot attention alternative source dECM. this work, novel biomimetic hydrogel is developed based on dECM obtained from mesenchymal stem cells (mdECM) cartilage engineering. To end, seeded under specific culture conditions generate early chondrogenic (ECM) providing cues elements...
Pancreatic ductal adenocarcinoma is the third leading cause of cancer-related death. Its dismal prognosis closely related to late diagnosis, which results from both non-specific symptoms and absence biomarkers for early diagnosis. MicroRNAs (miRNAs) exert a regulatory role in numerous biological processes their aberrant expression has been found broad spectrum diseases, including cancer. Cancer stem cells (CSCs) represent driving force PDAC initiation, progression meta-static spread. Our...
Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related death. Its poor prognosis closely related to late-stage diagnosis, which results from both nonspecific symptoms and absence biomarkers for early diagnosis. MicroRNAs (miRNAs) exert a regulatory role in numerous biological processes their aberrant expression has been found broad spectrum diseases, including cancer. Cancer stem cells (CSCs) represent driving force PDAC initiation, progression, metastatic...
Endothelial progenitor cells (EPC) represent a relatively rare cell population, and expansion of sufficient numbers remains challenge. Nevertheless, human adipose-derived stem (hASC) can be easily isolated possess the ability to differentiate into endothelial cells. Here, we propose isolation characterization multipotent endothelial-like (ME-LC) with capacity maintain their vascular properties for long periods. hASC were from lipoaspirates cultured through distinct consecutive culture stages...
Colorectal cancer (CRC) is a deadly tumour in Western countries characterized by high cellular/molecular heterogeneity. Cancer stem cells (CSC) act recurrence, drug-resistance and metastatic epithelial-to-mesenchymal transition. microRNAs (miRNAs) contribute to increasing, miRNA roles CSC phenotype fate their utility as CRC biomarkers have also been reported. Here, we investigated miR-21, miR-221, miR-18a, miR-210, miR-31, miR-34a, miR-10b miR-16 expression experimental ALDH+ CD44+/CD326+...
Abstract Tissue engineering (TE) seeks to fabricate implants that mimic the mechanical strength, structure, and composition of native tissues. Cartilage TE requires development functional personalized with cartilage‐like properties capable sustaining high load‐bearing environments integrate into surrounding tissue cartilage defect. In this study, we evaluated novel 1,4‐butanediol thermoplastic polyurethane elastomer (b‐TPUe) derivative filament as a 3D bioprinting material application in TE....
Abstract Autologous chondrocyte implantation (ACI) depends on the quality and quantity of implanted cells is hindered by fact that chondrocytes cultured for long periods time undergo dedifferentiation. Here we have developed a reproducible efficient chondrogenic protocol to redifferentiate isolated from osteoarthritis (OA) patients. We used morphological, histological immunological analysis together with RT-PCR detection collagen I II gene expression show articular cartilage biopsies...
Despite the great advances in cancer treatment, colorectal has emerged as second highest cause of death from worldwide. For this type tumor, use suicide gene therapy could represent a novel therapy. We recently demonstrated that co-expression gef and apoptin dramatically inhibits proliferation DLD-1 colon cell line. In present manuscript, we try to establish mechanism underlying enhanced induction apoptosis by triggering both expression tumor cells. Scanning microscopy reveals simultaneous...
Although education is among the major socioeconomic status (SES) resources that influence populations' and individuals' health, social marginalization may reduce health gain follows access to SES indicators such as education, a pattern called marginalization-related diminished return (MDR). The literature on MDRs, however, has been mainly derived from studies have defined based race, ethnicity, sexual orientation. Thus, more research needed MDRs follow result of immigration. To extend what...
The success of cell-based approaches for the treatment cartilage defects requires an optimal autologous cell source with chondrogenic differentiation ability that maintains its differentiated properties and stability following implantation.The objective this study was to compare capacity mesenchymal stem cells (MSCs) isolated from lipoaspirates (ASCs) infrapatellar fat pads (IFPSCs) osteoarthritic patients treated transforming growth factor (TGF)-β family-related factors.Cells were cultured...
The gef gene has cell-killing functions in Escherichia coli. To evaluate the feasibility of using this as a new strategy for cancer therapy, we transfected it MCF-7 cells derived from breast (MCF-7TG). was cloned pMAMneo vector under control mouse mammary tumour virus promoter, inducible by dexamethasone (Dex), and with liposomes. After selection induction, expression confirmed reverse transcription–polymerase chain reactions (RT–PCR) Western blot. Cell viability determined haemocytometre...
Malignant melanoma (MM) prognosis has been related to tumour thickness and clinical stage metastasis risk associated with presence of cells in peripheral blood. The aim this study was determine the relationship between tyrosinase blood MM patients their prognosis. Blood samples from 58 (stage I–IV) were analysed, using RT‐PCR assay detect mRNA. results showed that positive for significantly status thickness. After a median follow‐up 24 months, found be significant correlated recurrence ( p...