A5048323500- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- Autism Spectrum Disorder Research
- Chromosomal and Genetic Variations
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Neural dynamics and brain function
- Advanced biosensing and bioanalysis techniques
- DNA Repair Mechanisms
- Virus-based gene therapy research
- Genomic variations and chromosomal abnormalities
- Congenital heart defects research
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Single-cell and spatial transcriptomics
- Mosquito-borne diseases and control
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
University of California, San Diego
2016-2025
Rady Children's Hospital-San Diego
2016-2025
Orbital Research (United States)
2025
Sanford Consortium for Regenerative Medicine
2012-2024
Loma Linda University
2023
UC San Diego Health System
2019-2022
University of California System
2022
Cellular Research (United States)
2014-2020
Salk Institute for Biological Studies
2003-2017
Torrey Pines Institute For Molecular Studies
2016
Abstract The identification of mesenchymal stem cell (MSC) sources that are easily obtainable is utmost importance. Several studies have shown MSCs could be isolated from umbilical cord (UC) units. However, the presence in blood (UCB) controversial. A possible explanation for low efficiency UCB use different culture conditions by independent studies. Here, we compared obtaining unrelated paired and UC samples harvested same donors. Samples were processed simultaneously, under conditions....
Genetic reprogramming of somatic cells to a pluripotent state (induced stem or iPSCs) by over-expression specific genes has been accomplished using mouse and human cells. However, it is still unclear how similar iPSCs are Embryonic Stem Cells (hESCs). Here, we describe the transcriptional profile generated without viral vectors genomic insertions, revealing that these in general hESCs but with significant differences. For generation factors Oct4 Nanog were cloned episomal transfected into...
Significance Exosomes have been implicated in intercellular communication cancer and neurodegenerative disorders. We explored their function brain development. Proteomic analysis demonstrated that exosomes from isogenic control cultures contain neurodevelopmental signaling proteins, which are lacking MECP2 loss-of-function (MECP2LOF) cultures. Treating MECP2LOF neural with rescues deficits, increasing neurogenesis, synaptogenesis, network activity. similarly vivo: injecting purified into the...
Brain tumors are dynamic complex ecosystems with multiple cell types. To model the brain tumor microenvironment in a reproducible and scalable system, we developed rapid three-dimensional (3D) bioprinting method to construct clinically relevant biomimetic tissue models. In recurrent glioblastoma, macrophages/microglia prominently contribute mass. parse function of macrophages 3D, compared growth glioblastoma stem cells (GSCs) alone or astrocytes neural precursor hyaluronic acid-rich...
Significance Rett syndrome is a devastating neurodevelopmental disorder that currently has no cure. In this work, we demonstrate human neurons derived from patients with show significant deficit in neuron-specific K + -Cl − cotransporter2 (KCC2) expression, resulting delayed GABA functional switch. Restoring KCC2 level rescues deficits neurons. We further methyl CpG binding protein 2 regulates expression through inhibiting RE1-silencing transcriptional factor. Our data suggest potential...
Inherited variation contributes to autism About one-quarter of genetic variants that are associated with spectrum disorder (ASD) due de novo mutations in protein-coding genes. Brandler et al. wanted determine whether changes noncoding regions the genome autism. They applied whole-genome sequencing ∼2600 families at least one affected child. Children ASD had inherited structural from their father. Regulatory some specific genes were disrupted among multiple families, supporting idea a...