A5012827271- CRISPR and Genetic Engineering
- Diabetes and associated disorders
- T-cell and B-cell Immunology
- Photoreceptor and optogenetics research
- RNA regulation and disease
- Neural dynamics and brain function
- Asthma and respiratory diseases
- Drug-Induced Adverse Reactions
- Neuroscience and Neuropharmacology Research
- interferon and immune responses
- Neonatal Respiratory Health Research
- Congenital heart defects research
- Neurotransmitter Receptor Influence on Behavior
- Tryptophan and brain disorders
- Molecular Communication and Nanonetworks
- Sirtuins and Resveratrol in Medicine
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Tracheal and airway disorders
- Genetics, Aging, and Longevity in Model Organisms
- Genetic Neurodegenerative Diseases
- Autism Spectrum Disorder Research
- Peptidase Inhibition and Analysis
- Protein Tyrosine Phosphatases
- Stress Responses and Cortisol
Scripps (United States)
2022-2024
Scripps Institution of Oceanography
2022-2024
Scripps Research Institute
2021-2024
Scripps Health
2023
University of California, San Diego
2017-2019
Abstract Prefrontal circuits are thought to underlie aberrant emotion contributing relapse in abstinence; however, the discrete cell-types and mechanisms remain largely unknown. Corticotropin-releasing factor its cognate type-1 receptor, a prominent brain stress system, is implicated anxiety alcohol use disorder (AUD). Here, we tested hypothesis that medial prefrontal cortex CRF1-expressing (mPFC CRF1+ ) neurons comprise distinct population exhibits neuroadaptations following withdrawal from...
ADAR proteins alter gene expression both by catalyzing adenosine (A) to inosine (I) RNA editing and binding regulatory elements in target RNAs. Loss of ADARs affects neuronal function all animals studied date. Caenorhabditis elegans lacking exhibit reduced chemotaxis, but the targets responsible for this phenotype remain unknown. To identify critical neural C. elegans, we performed an unbiased assessment effects ADR-2, only A-to-I enzyme on transcriptome. Development implementation publicly...
The 1858C>T allele of the tyrosine phosphatase PTPN22 is present in 5–10% North American population and strongly associated with numerous autoimmune diseases. Although research has been done to define how this potentiates autoimmunity, influence its pro-autoimmune anti-viral immunity remains poorly defined. Here, we use single cell RNA-sequencing functional studies interrogate impact on during Lymphocytic Choriomeningitis Virus clone 13 (LCMV-cl13) infection. Mice homozygous for...
SUMMARY Structural and transcriptional changes during early brain maturation follow fixed developmental programs defined by genetics. However, whether this is true for functional network activity remains unknown, primarily due to experimental inaccessibility of the initial stages living human brain. Here, we developed cortical organoids that spontaneously display periodic regular oscillatory events are dependent on glutamatergic GABAergic signaling. These nested oscillations exhibit...
Fragile X Syndrome (FXS), the leading monogenic cause of intellectual disability and autism spectrum disorder, is caused by expansion a CGG trinucleotide repeat in 5'-UTR Mental Retardation-1 (FMR1) gene. Epigenetic silencing FMR1 results loss Retardation Protein (FMRP). Although most studies to date have focused on excitatory neurons, recent evidence suggests that GABAergic inhibitory networks are also affected. To investigate human neurogenesis, we established method reproducibly derive...
Type 2 alveolar epithelial cells (AEC2s) are stem in the adult lung that contribute to lower airway repair. Agents promote selective expansion of these might stimulate regeneration compromised epithelium, an etiology-defining event several pulmonary diseases. From a high-content imaging screen drug repurposing library ReFRAME, we identified dipeptidyl peptidase 4 (DPP4) inhibitors, widely used type diabetes medications, selectively expand AEC2s and broadly efficacious mouse models damage....
Introduction: Friedreich ataxia (FRDA) is a recessive neurodegenerative disease characterized by progressive ataxia, dyscoordination, and loss of vision. The variable length the pathogenic GAA triplet repeat expansion in FXN gene part explains interindividual variability severity disease. leads to epigenetic silencing FXN; therefore, properties effector proteins could also regulate FRDA. Methods: In an exploratory analysis, DNA from 88 individuals with FRDA was analyzed determine if any five...
Abstract The 1858C>T allele of the tyrosine phosphatase PTPN22 is present in 5-10% North American population and strongly associated with numerous autoimmune diseases. Although research has been done to define how this potentiates autoimmunity, influence its pro-autoimmune anti-viral immunity remains poorly defined. Here, we use single cell RNA- sequencing functional studies interrogate impact pro- on during Lymphocytic Choriomeningitis Virus clone 13 (LCMV-cl13) infection. Mice...