A5103127346- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Hepatitis B Virus Studies
- Neuroscience and Neuropharmacology Research
- Hepatitis C virus research
- RNA Research and Splicing
- Circular RNAs in diseases
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Mycobacterium research and diagnosis
- Ubiquitin and proteasome pathways
- Parkinson's Disease Mechanisms and Treatments
- Autoimmune Neurological Disorders and Treatments
- Autophagy in Disease and Therapy
- DNA Repair Mechanisms
- Cancer, Hypoxia, and Metabolism
- Lipid metabolism and disorders
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Sirtuins and Resveratrol in Medicine
- Plant-derived Lignans Synthesis and Bioactivity
- Lung Cancer Diagnosis and Treatment
Children's Hospital of Philadelphia
2004-2024
Zhengzhou University
2021-2024
University of Pennsylvania
2021-2024
Jilin Province Tumor Hospital
2021-2024
Renmin Hospital of Wuhan University
2017-2023
Wuhan University
2017-2023
Beijing Obstetrics and Gynecology Hospital
2019-2022
Capital Medical University
2019-2022
Chengde Medical University
2022
Swiss Data Science Center
2022
Pyroptosis induced by lipopolysaccharide (LPS) is a dissolved form of cell death. The molecular marker gasdermin D, specifically GSDMD-N, critically required for the induction pyroptosis. Recently, there have been studies showing that LPS closely related to tumor biology.Specimens from 40 patients with colorectal cancer (CRC) were collected. Eight- twelve-week-old C57BL6 male mice (n=30) raised. Immunohistochemistry and Western blot performed test expression GSDMD. Moreover, cytotoxicity...
ABSTRACT Friedreich ataxia (FRDA), the most common recessive inherited ataxia, results from deficiency of frataxin, a small mitochondrial protein crucial for iron-sulphur cluster formation and ATP production. Frataxin is associated with dysfunction in FRDA patients animal models; however, early pathology cerebellum remains elusive. Using frataxin knock-in/knockout (KIKO) mice KIKO carrying mitoDendra transgene, we show cerebellar deficits biogenesis respiratory chain complexes this model. At...
Objectives/Aims This is an open-label trial of the safety interferon gamma-1b (IFN-γ) and its effect on frataxin levels neurologic measures in 12 children with Friedreich ataxia. Materials Methods Interferon was administered via subcutaneous injection three times weekly. The dose increased from 10 to 50 mcg/m2 during first four weeks then remained at for final eight weeks. Safety assessments included laboratory testing, electrocardiogram, monitoring adverse events. primary efficacy outcome...
Friedreich ataxia (FRDA), the most common autosomal recessive ataxia, is characterized by degeneration of large sensory neurons and spinocerebellar tracts, cardiomyopathy, increased incidence in diabetes. The underlying pathophysiological mechanism FRDA, driven a significantly decreased expression frataxin (FXN), involves oxidative stress, reduced activity enzymes containing iron‑sulfur clusters (ISC), defective energy production, calcium dyshomeostasis, impaired mitochondrial biogenesis,...
The calcium-dependent protease calpain cleaves the NMDA receptor 2 (NR2) subunit of both in vitro and vivo thus potentially modulates function turnover. We examined ability postsynaptic density-95 (PSD-95) protein to alter calpain-mediated cleavage NR2A NR2B. Coexpression PSD-95 with receptors human embryonic kidney 293 cells blocked NR2B by receptor-activated calpain. occurred cell surface intracellular fractions required presence NR1 subunits. blocking effect did not result from decreased...
Amlexanox has been developed as a 5 percent topical oral paste for the treatment of patients with recurrent aphthous stomatitis (RAS) in most European countries. However, it is not yet available China and generally accepted clinical treatment. The aim this study was to explore effectiveness amlexanox adhesive pellicles minor ulcers, compare results those tablets order analyse difference between two dosage forms amlexanox.We performed randomized, blinded, placebo-controlled, parallel,...
Abstract Objective In vitro, in vivo, and open‐label studies suggest that interferon gamma ( IFN ‐ γ 1b) may improve clinical features Friedreich Ataxia through an increase frataxin levels. The present study evaluates the efficacy safety of 1b treatment a double‐blind, multicenter, placebo‐controlled trial. Methods Ninety‐two subjects with FRDA between 10 25 years age were enrolled. Subjects received either or placebo for 6 months. primary outcome measure was modified Rating Scale mFARS )....
Patients with chronic hyperglycemia are at high risk of developing diabetic retinopathy. In this study, we investigated the functional role long-noncoding RNA (lncRNA) X-inactive specific transcript (XIST) in anin vitro model human retinal pigment epithelial ARPE-19 cells.ARPE-19 cells were cultured normal glucose (NG) and high-glucose (HG) conditions to mimic hyperglycemia-associated cell apoptosis, migration XIST expression. was overexpressed examine its functions HG-induced apoptosis...
Friedreich's ataxia (FRDA) is an autosomal recessive neurodegenerative disease caused by the deficiency of mitochondrial protein frataxin, which plays a crucial role in iron-sulphur cluster formation and ATP production. The cellular function frataxin not entirely known. Here, we demonstrate that controls ketone body metabolism through regulation 3-Oxoacid CoA-Transferase 1 (OXCT1), rate limiting enzyme catalyzing conversion bodies to acetoacetyl-CoA then fed into Krebs cycle. Biochemical...
More than half of all cancers demonstrate aberrant c-Myc expression, making this arguably the most important human oncogene. Deregulated long non-coding RNAs (lncRNAs) are also commonly implicated in tumorigenesis, and some limited examples have been established where lncRNAs act as biological tuners expression activity. Here, we that lncRNA denoted Enhancing Factor (MEF) enjoys a cooperative relationship with c-Myc, both transcriptional target driver expression. Mechanistically, MEF...
Abstract NMDA receptors play critical roles in synaptic modulation and neurological disorders. In this study, we investigated the developmental changes NR2 cleavage by receptor‐activated calpain cultured cortical hippocampal neurons. Calpain activity increased with development, associated expression of but not I. The activation immature mature cultures was inhibited antagonists NR1/2B NR1/2A/2B receptors, whereas inhibition did alter cultures. degradation subunits differed age. NR2A a...
Abstract Emerging evidence suggests that long noncoding RNAs (lncRNAs) are involved in many biological processes, such as cell growth, differentiation, apoptosis, and autophagy. Metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1), highly expressed endothelial cells, is well conserved implicated migration proliferation. However, whether MALAT1 participates oxidized low‐density lipoprotein (ox‐LDL)‐induced autophagy regulation human umbilical vein cells (HUVECs) remains unknown. In...
Click to increase image sizeClick decrease sizeView retraction statement:Statement of Retraction: Salidroside alleviates high-glucose-induced injury in retinal pigment epithelial cell line ARPE-19 by down-regulation miR-138
N-methyl-D-aspartate (NMDA) receptors are widely expressed in the central nervous system. However, their presence and function at extraneuronal sites is less well characterized. In present study, we examined expression of NMDA receptor subunit mRNA protein human pulmonary artery (HPA) by quantitative polymerase chain reaction (PCR), immunohistochemistry immunoblotting. We demonstrate that both GluN1 GluN2 mRNAs HPA. addition, (A-D) proteins smooth muscle cells (HPASMCs) vitro vivo. These...
Gemcitabine-based chemotherapy is commonly applied for the treatment of breast cancer in a clinical setting. However, acquired resistance to primarily results failure and eventually culminates patient mortality. Aberrant expression microRNAs (miRNAs) has been demonstrated be implicated development chemoresistance; however, role miR-873 chemoresistance its underlying mechanism have not completely elucidated. Herein, using cell viability assays, present study that overexpression sensitized...
Friedreich ataxia (FRDA) is a neurodegenerative disease caused by GAA triplet repeat (GAA-TR) expansion in intron 1 of the FXN gene. Patients have 100-1,300 triplets compared with less than 30 healthy controls. The GAA-TR leads to silencing, and consequent frataxin protein deficiency results progressive ataxia, scoliosis, cardiomyopathy, diabetes. overt heterogeneity age at onset severity explained partly length GAA-TR, which shorter repeats correlate milder disease. Evidence variegated...