A5077478635- Platelet Disorders and Treatments
- Heparin-Induced Thrombocytopenia and Thrombosis
- Blood Coagulation and Thrombosis Mechanisms
- Protease and Inhibitor Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Blood groups and transfusion
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- Acute Ischemic Stroke Management
- Intramuscular injections and effects
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Autoimmune Bullous Skin Diseases
- S100 Proteins and Annexins
- Complement system in diseases
- Blood disorders and treatments
- Monoclonal and Polyclonal Antibodies Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Antiplatelet Therapy and Cardiovascular Diseases
- Peptidase Inhibition and Analysis
- Antimicrobial Peptides and Activities
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Erythrocyte Function and Pathophysiology
- Proteoglycans and glycosaminoglycans research
- Systemic Lupus Erythematosus Research
- Neonatal and fetal brain pathology
University of Pennsylvania
2016-2025
Cornell University
1991-2024
Weill Cornell Medicine
2021-2024
Technion – Israel Institute of Technology
2021
Hillel Yaffe Medical Center
2021
California University of Pennsylvania
2004-2020
Philadelphia University
1991-2017
Faculty (United Kingdom)
2016
Hebrew University of Jerusalem
2005-2016
University of Nicosia
2013
Abstract Background: Despite an increase in the number of therapies available to treat patients with immune thrombocytopenia (ITP), there are minimal data from randomized trials assist physicians management patients. Objective: These evidence-based guidelines American Society Hematology (ASH) intended support patients, clinicians, and other health care professionals their decisions about ITP. Methods: In 2015, ASH formed a multidisciplinary guideline panel that included 8 adult clinical...
We studied the possibility that immune injury to endothelial cells may have a role in development of thrombosis some patients with heparin-associated thrombocytopenia. Serum samples from each 27 who had this clinical diagnosis contained heparindependent platelet antibodies and deposited more than normal amounts IgG, IgA, or IgM on cells, stimulating production tissue factor. Binding immunoglobulins was no longer detected when were after heparin withdrawn, but reappeared within several days...
Cases of apparent secondary immune thrombocytopenia (ITP) after SARS-CoV-2 vaccination with both the Pfizer and Moderna versions have been reported reached public attention. Public alarm was heightened following death first identified patient from an intracranial hemorrhage, which on Internet, then in USA Today1 The New York Times.2 Described below, we collected a series cases very low platelet counts occurring within 2 weeks order to enhance our understanding possible relationship, if any,...
We applied a Radio-labeled Coombs antiglobulin test to the diagnosis and management of immune thrombocytopenia in adults children. This assay substantiated that majority patients with idiopathic thrombocytopenic purpura have increased levels IgG on their platelets. Platelet-associated C3 was elevated subset these patients, some whom had normal platelet-associated IgG, thus suggesting role for pathogenesis thrombocytopenia. The enabled us anticipate change clinical status, establish presence...
Abstract Although arterial and venous thromboembolic disorders are among the most frequent causes of mortality morbidity, there has been little description how composition thrombi emboli depends on their vascular origin age. We quantified structure pulmonary using high-resolution scanning electron microscopy. Arterial contained a surprisingly large amount fibrin, in addition to platelets. The mirrored distal part from which they originated, differed body head same thrombi. All few biconcave...
Within the first months of COVID-19 vaccination campaign, previously healthy recipients who developed severe thrombosis (often cerebral and/or splanchnic vasculature) and thrombocytopenia typically after adenoviral vector-based were identified. Similarities between this syndrome, vaccine-induced immune thrombotic (VITT), heparin-induced prompted recognition role antiplatelet factor 4 (PF4) antibodies management strategies based on IV immunoglobulin nonheparin anticoagulants, which improved...
Patients with cardiovascular disease almost invariably receive heparin before cardiopulmonary bypass surgery, which places them at risk of developing heparin-associated antibodies a thromboembolic complications. This study was designed to determine the prevalence heparin-induced in patients and after surgery.Plasma from 111 tested surgery 5 days for heparin-dependent platelet-reactive 14C-serotonin-release assay (SRA) heparin/platelet factor 4 complexes an ELISA. Heparin exposure minimized....
The urokinase plasminogen activator binds to its cellular receptor with high affinity and initiates signaling cascades that are implicated in pathological processes including tumor growth, metastasis, inflammation. We report the crystal structure at 1.9 angstroms of complexed amino-terminal fragment an antibody against receptor. three domains form a concave shape central cone-shaped cavity where inserts. provides insight into flexibility enables interaction wide variety ligands basis for...
Fibrinogen binding to receptors on stimulated platelets is a prerequisite for platelet aggregation. To gain further insight into the role of fibrinogen in aggregation and identify receptor, we developed monoclonal anti-platelet antibody that inhibited The purified antibody, designated A2A9, by 10 microM ADP, epinephrine, thrombin at 1 unit/ml without inhibiting shape change or secretion. A2A9 was also competitive inhibitor ADP-stimulated platelets. Fifty percent inhibition occurred 65 nM...
We studied the mechanism of platelet injury in 20 patients receiving heparin, three whom became thrombocytopenic. Platelets from these had increased levels IgG and C3, which correlated with presence thrombocytopenia; their plasma caused release serotonin normal platelets at concentrations heparin within usual therapeutic range. This reaction required an intact classic complement pathway. The 17 thrombocytopenia did not develop platelet-associated C3. Plasma 14 serotonin, but only above...
Vasculitis in systemic lupus erythematosus (SLE) is associated with the deposition of IgG and complement blood vessel walls. However, it not known whether immune injury to endothelial cells a part this process. Therefore, we used solid phase radioimmunoassay study ability from normal human sera patients SLE bind cells. In assay, cultured umbilical venous were sequentially incubated or sera, goat anti-human IgG, 125I-labeled staphylococcal protein A (*SPA). After exposure 2.5 +/- 0.5% (mean...