A5084190090- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Renal and related cancers
- Neurogenesis and neuroplasticity mechanisms
- Genetic and Kidney Cyst Diseases
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Congenital heart defects research
- Planarian Biology and Electrostimulation
- Epigenetics and DNA Methylation
- Fetal and Pediatric Neurological Disorders
- Autism Spectrum Disorder Research
- Neural dynamics and brain function
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Heat shock proteins research
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Hedgehog Signaling Pathway Studies
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Neuroscience and Neuropharmacology Research
MRC Laboratory of Molecular Biology
2016-2025
University of Cambridge
2022-2025
Wellcome/MRC Cambridge Stem Cell Institute
2020-2025
Medical Research Council
2015-2024
The Francis Crick Institute
2023
Institute of Molecular Biotechnology
2011-2017
Austrian Academy of Sciences
2011-2017
Vienna Biocenter
2017
Howard Hughes Medical Institute
2010-2011
University of California, San Diego
2006-2011
Cerebral organoids-3D cultures of human cerebral tissue derived from pluripotent stem cells-have emerged as models cortical development. However, the extent to which in vitro organoid systems recapitulate neural progenitor cell proliferation and neuronal differentiation programs observed vivo remains unclear. Here we use single-cell RNA sequencing (scRNA-seq) dissect compare composition progenitor-to-neuron lineage relationships organoids fetal neocortex. Covariation network analysis using...
Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, leads to symptoms that can be fatal. However, neurological have also been observed in some patients. The cause of these complications is currently unknown. Here, we use human-pluripotent-stem-cell-derived brain organoids examine SARS-CoV-2 neurotropism. We find expression viral receptor ACE2 mature choroid plexus cells expressing abundant lipoproteins, but not neurons or...
Cerebrospinal fluid (CSF) is a vital liquid, providing nutrients and signaling molecules clearing out toxic by-products from the brain. The CSF produced by choroid plexus (ChP), protective epithelial barrier that also prevents free entry of or drugs blood. Here, we establish human ChP organoids with selective CSF-like secretion in self-contained compartments. We show this vitro exhibits same selectivity to small as vivo ChP-CSF can predict central nervous system (CNS) permeability new...
Organoids derived from human pluripotent stem cells recapitulate the early three-dimensional organization of brain, but whether they establish epigenomic and transcriptional programs essential for brain development is unknown. We compared regulatory features in cerebral organoids fetal using genome-wide, base resolution DNA methylome transcriptome sequencing. Transcriptomic dynamics faithfully modeled gene expression trajectories early-to-mid brains. found that non-CG methylation...
Model organisms are widely used in research as accessible and convenient systems to study a particular area or question biology. Traditionally only handful of have been studied, but modern tools enabling researchers extend the set model include less-studied more unusual systems. This Forum highlights range 'non-model organisms' emerging for tackling questions across whole spectrum biology (and beyond), opportunities challenges, outlook future.
The human brain has undergone rapid expansion since humans diverged from other great apes, but the mechanism of this human-specific enlargement is still unknown. Here, we use cerebral organoids derived human, gorilla, and chimpanzee cells to study developmental mechanisms driving evolutionary expansion. We find that neuroepithelial differentiation a protracted process in involving previously unrecognized transition state characterized by change cell shape. Furthermore, show are larger due...
The substantial phenotypic heterogeneity in autism limits our understanding of its genetic etiology. To address this gap, here we investigated differences between autistic individuals (n