A5015754489- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Mesenchymal stem cell research
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- Lung Cancer Research Studies
- Dental materials and restorations
- Tissue Engineering and Regenerative Medicine
- SARS-CoV-2 and COVID-19 Research
- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- MicroRNA in disease regulation
- COVID-19 Clinical Research Studies
- Dental Radiography and Imaging
- Microfluidic and Capillary Electrophoresis Applications
- Nanoparticle-Based Drug Delivery
- Alkaline Phosphatase Research Studies
- Digital Imaging in Medicine
- Electrical and Bioimpedance Tomography
- Conservation Techniques and Studies
- Curcumin's Biomedical Applications
- Virus-based gene therapy research
- Characterization and Applications of Magnetic Nanoparticles
- Cell Adhesion Molecules Research
University of Twente
2014-2025
Saxion University of Applied Sciences
2020
Radboud University Nijmegen
1993-1998
In differentiated cells, aging is associated with hypermethylation of DNA regions enriched in repressive histone post-translational modifications. However, the chromatin marks changes methylation adult stem cells during lifetime are still largely unknown. Here, profiling mesenchymal (MSCs) obtained from individuals aged 2 to 92 yr identified 18,735 hypermethylated and 45,407 hypomethylated CpG sites aging. As sequences were marks. Most importantly, strongly active mark H3K4me1 suggesting...
Plasma treatment is a method to modify the physicochemical properties of biomaterials, which consequently may affect interactions with cells.Based on rationale that physical cues surface culture substrates and implants, such as roughness, have proven alter cell behaviour, we used electrospinning fabricate fibrous three-dimensional scaffolds made poly (ethylene oxide terephthalate)/poly (butylene terephthalate) copolymer mimic microenvironment extracellular matrix applied radio-frequency...
Human aging is associated with loss of function and regenerative capacity. bone marrow derived mesenchymal stromal cells (hMSCs) are involved in tissue regeneration, evidenced by their capacity to differentiate into several lineages therefore considered the golden standard for cell-based regeneration therapy. Tissue maintenance dependent on stem declines age thought influence therapeutic efficacy, therefore, more insight process hMSCs high interest. We, hypothesized that might reflect signs...
Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL blood is too small to reliably determine heterogeneity and be representative as a “liquid biopsy”. In EU FP7 program CTCTrap, we aimed validate optimize recently introduced Diagnostic LeukApheresis (DLA) screen liters blood. Here present results obtained from 34 metastatic cancer patients subjected DLA participating institutions. About processed with CellSearch® was used “gold standard” reference. DLAs were 22...
In this retrospective study 516 teeth restored with a cast post and core build‐up were followed from 1970 till 1990. The data was derived the dental records of 283 clinic patients treated by senior students. survival rate found to be 82% after 10 years for cores in anterior region. most frequent failure characteristic recementation (46%), rerestoration (32%). solitary provisions posterior showed relative high rate, compared other tooth‐types locations.
Human multipotent mesenchymal stromal cells (hMSCs) are that, in the presence of appropriate stimuli, can differentiate into different lineages such as osteogenic, chondrogenic, and adipogenic lineages. In ascorbic acid, MSCs secrete an extracellular matrix mainly composed collagen type I. Here we assessed potential role endogenous synthesis hMSC differentiation stem cell maintenance. We observed a sharp reduction proliferation rate hMSCs absence concomitant with osteogenesis vitro bone...
Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding IgG, IgM IgA against Receptor Binding Domain (RBD) SARS-CoV-2 in sera 119 CoViD-19 patients. The SPRi assay measures antibody isotype levels RBD ultimate 384 patient samples one run. It turns out that during course disease, IgG increased while generally go down. Recovered patients all show high type protein. anti-RBD immunoglobulins provides additional insights immune status recovering from...
Detecting circulating tumor cells (CTCs) is challenging due to their low presence and heterogeneity. Traditional methods using EpCAM‐based separation struggle with CTCs that have undergone epithelial‐mesenchymal transition, as this results in lower EpCAM expression. This study presents the use of silica‐coated magnetic nanobeads functionalized streptavidin for CTC capture. Using FETCH system, we validated capture efficiency our beads on varying Our showed superior rates LNCaP (97%), PC3‐9...
The presence of tumor cells in blood is predictive short survival several cancers and their isolation characterization can guide toward the use more effective treatments. These circulating (CTC) are, however, extremely rare require a technology that sufficiently sensitive specific to identify CTC against background billions cells. Immuno-capture expressing epithelial cell adhesion molecule (EpCAM) are frequently used enrich from blood. choice bio conjugation strategy antibody clone crucial...
Due to the low frequency of circulating tumor cells (CTC), standard CellSearch method enumeration and isolation using a single tube blood is insufficient measure treatment effects consistently, or steer personalized therapy. Using diagnostic leukapheresis this sample size can be increased; however, also calls for suitable new process larger inputs. In order achieve this, we have optimized immunomagnetic enrichment flow-through magnetophoretic system. An overview major forces involved in...
Bone marrow-derived multipotent mesenchymal stromal cells (MSCs) are the most frequently investigated cell type for potential regenerative strategies because they relatively easy to isolate and able differentiate into several lineages. Unfortunately, during ex vivo culture, MSCs present gradual loss of differentiation reduced clinical efficacy. Reactive oxygen species (ROS) associated with oxidative damage accumulate MSC expansion. Because ROS believed be involved in multipotency, we...
Application of autologous cells is considered for a broad range regenerative therapies because it not surrounded by the immunological and ethical issues allo- or xenogenic cells. However, isolation, expansion, application do suffer from variability in therapeutic efficacy due to donor differences prolonged culture. One important source mesenchymal stromal (MSCs), which can differentiate toward endothelial-like cells, thus making them an ideal candidate as cell tissue vascularization. Here we...
Summary During the period 1974‐1986, 112 post and core build‐ups were inserted in 74 patients. The consisted of a metal prefabricated (Dentatus, Unimetric or Radix) combination with composite core. After an average follow‐up 7.9 years, 14 failures (12.5%) noticed. Eight teeth could be rerestored six had to extracted. However, when correction was made for bad initial prognosis extractions not related build‐up restoration, eight remained caused by failure (7.5%). Dentatus posts seemed increase...
When evaluating EpCAM-based enrichment technologies for circulating tumour cells (CTCs), the cell lines used should closely resemble real CTCs, meaning EpCAM expression of CTCs needs to be known, but also at different institutions and times is important. As number in blood low, we enriched through depletion leukocytes from diagnostic leukapheresis products 13 prostate cancer patients measured using quantitative flow cytometry. Antigen was compared between multiple by measuring cultures each...
Human mesenchymal stem cells (hMSCs) are being considered for several areas of clinical therapy, due to their multipotent nature. For instance, osteogenic hMSCs applied in bone tissue engineering, but current differentiation protocols need further optimization before they can be clinically applied. Protein kinase C (PKC) family members have been implicated metabolism, which prompted us use a pharmaceutical approach manipulate PKC signalling hMSCs. Inhibition resulted dose-dependent...
Here we presented a set-up which can be used to capture cancer cells in continues flow fusing functionalized surface.
Since the discovery that bone morphogenetic proteins (BMPs) are able to induce ectopic formation, considerable effort has been devoted apply it for regeneration. BMP activity needs be temporally and spatially controlled organism devised ways achieve it. Here we show inhibitor chordin-like 1 can interfere with BMP2 signalling thereby affecting osteogenic differentiation of MC3T3-E1 cells. Besides its function as a antagonist, enhanced proliferation human mesenchymal stem cells (hMSCs) in...
Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known be important initiation responses, studies about FRCs their interactions have been impeded because are limited availability lose function upon culture expansion. To circumvent these limitations, precursors can mechanotranduced form mature FRCs. Here, we used library designed surface...
Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding IgG, IgM IgA against Receptor Binding Domain (RBD) SARS-CoV-2 in sera 102 CoViD-19 non-CoViD-19 patients. The SPRi assay simultaneously measures antibody isotype levels RBD ultimate 384 patient samples one run. It turns out that during course disease, IgG increased while generally go down. Recovered patients all show high type protein. anti-RBD immunoglobulins provides additional insights...