Abstract Biofabrication commonly involves the use of liquid droplets to transport cells printed structure. However, viability after impact is poorly controlled and understood, hampering applications including cell spraying, inkjet bioprinting laser-assisted transfer. Here, we present an analytical model describing as a function cell-surrounding droplet characteristics. The connects (1) survival membrane elongation, (2) elongation cell-containing size velocity (3) substrate properties....

The multi-directional mechanical stimulation experienced by cartilage during motion is transferred to chondrocytes, which respond releasing matrix proteins and/or matrix-degrading enzymes.

Mesenchymal stem cells (MSCs) are multipotent cells, mainly from bone marrow, and an ideal source of in cartilage tissue engineering. A study the chondrogenic differentiation MSCs is particular interest for MSCs-based regeneration. In this study, we aimed to optimize conditions chrondogenic by regulating WNT signaling using small molecule inhibitor PKF118-310 activator BIO. Human mesenchymal (hMSCs) were isolated marrow aspirates cultured hMSCs proliferation medium. Pellet culture was...

Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding IgG, IgM IgA against Receptor Binding Domain (RBD) SARS-CoV-2 in sera 119 CoViD-19 patients. The SPRi assay measures antibody isotype levels RBD ultimate 384 patient samples one run. It turns out that during course disease, IgG increased while generally go down. Recovered patients all show high type protein. anti-RBD immunoglobulins provides additional insights immune status recovering from...

Background Almost 2 years from the beginning of coronavirus disease 2019 (COVID-19) pandemic, there is still a lot unknown how humoral response affects progression. In this study, we investigated antibody responses against specific SARS-CoV2 proteins, their strength binding, and relationship with COVID severity clinical information. Furthermore, studied interactions receptor-binding domain (RBD) in more depth by characterizing to peptide library. Materials Methods We measured antibodies...

This report describes a novel system to create rapid prototyped 3-dimensional (3D) fibrous scaffolds with shell-core fiber architecture in which the core polymer supplies mechanical properties and shell acts as coating providing desired physicochemical surface properties. Poly[(ethylene oxide) terephthalate-co-poly(butylene) terephthalate] (PEOT/PBT) 3D deposited (3DF) were fabricated examined for articular cartilage tissue regeneration. The contained higher molecular weight of initial...

Abstract Spatiotemporal control over engineered tissues is highly desirable for various biomedical applications as it emulates the dynamic behavior of natural tissues. Current spatiotemporal biomaterial functionalization approaches are based on cytotoxic, technically challenging, or non-scalable chemistries, which has hampered their widespread usage. Here we report a strategy to spatiotemporally functionalize (bio)materials competitive supramolecular complexation avidin and biotin analogs....

The purpose of this study was to make an explicit test the idea that a retinoid could act as morphogen, differentially activating genes and specifying anteroposterior (a-p) level in developing vertebrate central nervous system (CNS). Our approach characterize concentration-dependent effects retinoic acid (RA) on neural expression set a-p patterning genes, both vivo vitro for patterning. results indicate is unlikely specify along entire CNS. Instead, our data support hindbrain may be...

To investigate the presence of WNT antagonists Dickkopf-related protein 1 (DKK1), Frizzled-related (FRZB) and BMP antagonist Gremlin (GREM1) in synovial fluid (SF) serum, respectively, from end-stage knee osteoarthritis (OA) patients, correlate their expression with other markers OA.In a cross-sectional study, SF serum were collected OA patients (n = 132). The concentrations DKK1, FRZB GREM1 determined using immunoassays. Correlation measurements performed between groups previously assessed...

There is a large unmet need for reliable biomarker measurement systems clinical application. Such should meet challenging requirements scale use, including dynamic detection range, multiplexing capacity, and both high specificity sensitivity. More importantly, these to apply complex biological samples, which require extensive quality control. In this paper, we present the development of an enhancement cascade surface plasmon resonance imaging (SPRi). The applies antibody sandwich assay,...

Cells respond to their environment via an intricate cellular signaling network, directing cell fate. Changes in fate are characterized by changes gene transcription, dictated (master) transcription factor activity. SOX9 is the master for chondrocyte development. Its impaired function implicated osteoarthritis and growth disorders, such as dwarfism. However, factors regulating transcriptional activity not yet fully mapped. Current methods study indirect largely limited quantification of...

Airbrush-assisted cell spraying would facilitate fully arthroscopic filling of cartilage defects, thereby providing a minimally invasive procedure for repair. This study provides the development and characterization custom-made spray nozzles that could serve as foundation BioAirbrush, platform technology application (cell laden) hydrogels.Custom-made were designed produced with 3D printing technology. A commercially available system was used comparison. Sprays characterized based on angle,...

Due to its avascular nature, articular cartilage is relatively hypoxic. The aim of this study was elucidate the functional changes macroscopically healthy looking areas chondrocytes (MHC) and damaged regions (MDC) at a cellular level in response inflammatory cytokine IL1β under different oxygen tension levels. In study, two-dimensional (2-D) expanded MHC MDC were redifferentiated 3-D pellet cultures chondrogenic differentiation medium, supplemented with or without conventional culture...

Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding IgG, IgM IgA against Receptor Binding Domain (RBD) SARS-CoV-2 in sera 102 CoViD-19 non-CoViD-19 patients. The SPRi assay simultaneously measures antibody isotype levels RBD ultimate 384 patient samples one run. It turns out that during course disease, IgG increased while generally go down. Recovered patients all show high type protein. anti-RBD immunoglobulins provides additional insights...

Abstract Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding IgG, IgM IgA against Receptor Binding Domain (RBD) SARS-CoV-2 in sera 119 CoViD-19 patients. The high-throughput assay enables follow specific immune response ultimate 384 individuals for these four parameters one run. measured levels correlated with ELISA (Euroimmun: Anti-SARS-CoV-2, IgG assay, r-0.95, ECLIA: Anti-SARS-CoV-2 Ig electrochemiluminescence r=0.73). During course disease,...

Biomarker measurements are essential for the early diagnosis of complex diseases. However, many current biomarker assays lack sensitivity and multiplexing capacity, work in a narrow detection range importantly real time quality control opportunities, which hampers clinical translation. In this paper, we demonstrate toolbox to kinetically characterize measurement assay using Surface Plasmon Resonance imaging (SPRi) with ample opportunities by exploiting quantitative descriptions various...