A5004149712- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- 3D Printing in Biomedical Research
- Total Knee Arthroplasty Outcomes
- Tendon Structure and Treatment
- Muscle activation and electromyography studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer-related molecular mechanisms research
- Orthopedic Surgery and Rehabilitation
- Prosthetics and Rehabilitation Robotics
- Autophagy in Disease and Therapy
- Wireless Body Area Networks
- Molecular Communication and Nanonetworks
- Conducting polymers and applications
- RNA modifications and cancer
- Lubricants and Their Additives
- Mesenchymal stem cell research
- Additive Manufacturing and 3D Printing Technologies
- Cancer-related gene regulation
- Orthopaedic implants and arthroplasty
- Cellular Mechanics and Interactions
- Advanced Biosensing Techniques and Applications
- Silk-based biomaterials and applications
- Neuroscience and Neural Engineering
- RNA regulation and disease
University of Twente
2018-2024
Bioengineering Center
2018-2021
Chan Heart Rhythm Institute
2021
Mayo Clinic in Arizona
2019-2020
Mayo Clinic
2019
WinnMed
2019
The multi-directional mechanical stimulation experienced by cartilage during motion is transferred to chondrocytes, which respond releasing matrix proteins and/or matrix-degrading enzymes.
Movement is essential to our quality of life, and regulates cell behavior via mechanical stimulation. Here, we report a monolithic microfluidic platform, in which engineered tissues composed cells hydrogel are exposed gradients compression. Mechanical stimulation applied through the deflection thin polydimethylsiloxane (PDMS) vertical membrane. The device design all actuation parameters were optimized this work produce physiologically relevant compression on cartilage model (strain 5–12 %),...
Objective This study aims to (1) determine and validate living cartilage allograft transplantation as a novel source for viable osteochondral (OCA) tissues (2) perform histologic viability comparisons of donor currently available clinical-grade standard processed grafts. Design Using healthy from well-preserved contralateral compartments in 27 patients undergoing total knee arthroplasty (TKA) 10 OCA specimens obtained immediately following operative implantation, quality was evaluated at the...
Objective. Osteochondral allograft (OCA) transplantation has demonstrated good long-term outcomes in treatment of cartilage defects. Viability, a key factor clinical success, decreases with peri-implantation storage at 4°C during pathogen testing, matching logistics, and transportation. Modern, physiologic conditions may improve viability enhance outcomes. Design. specimens from total knee arthroplasty patients (6 males, 5 females, age 56.4 ± 2.2 years) were stored media incubated normoxia...
Abstract The vascular tree is crucial for the survival and function of large living tissues. Despite breakthroughs in 3D bioprinting to endow engineered tissues with blood vessels, there currently no approach engineer high‐density capillary networks into a scalable manner. Here, photoannealing microtissue (PALM) presented as strategy capillary‐rich Specifically, in‐air microfluidics used produce microtissues composed cell‐laden microgels ultrahigh throughput, which can be photoannealed...
Objective Cartilage formation is stimulated in mixtures of chondrocytes and human adipose–derived mesenchymal stromal cells (MSCs) both vitro vivo. During coculture, MSCs perish. The goal this study to elucidate the mechanism by which adipose tissue–derived MSC cell death occurs presence chondrocytes. Methods Human primary were cocultured with derived from 3 donors. cultured monoculture or coculture (20% 80% MSCs) pellets (200,000 cells/pellet) for 7 days chondrocyte proliferation media...
Anterior cruciate ligament (ACL) injuries are frequent, as >200,000 occur in the United States alone each year. Owing to risks for associated meniscus and cartilage damage, ACL a significant source of both orthopedic care research. Given extended recovery course after injury, which often lasts 1-2 years, is with limited participation sports activities daily living patients, there critical need evolution new improved methods repair. Subsequently, animal models reconstruction (ACLR) play key...
Abstract Osteoarthritis (OA) is one of the most prevalent joint diseases globally, characterized by progressive breakdown articular cartilage, resulting in chronic pain, stiffness, and loss function. Despite its significant socioeconomic impact, therapeutic options remain limited, largely due to an incomplete understanding molecular mechanisms driving cartilage degradation OA pathogenesis. Recent advances vitro modeling have revolutionized tissue research, transitioning from simplistic...
Supplemental material, supinfofinalfinalversion_adapted_for_resub_complete for Autophagy Is Involved in Mesenchymal Stem Cell Death Coculture with Chondrocytes by Carlo Alberto Paggi, Amel Dudakovic, Yao Fu, Catalina Galeano Garces, Mario Hevesi, Daniela Allan B. Dietz, Andre van Wijnen and Marcel Karperien CARTILAGE
IntroductionImproving ligament reconstruction biology may potentially be achieved through capturing autologous circulating factors such as vascular endothelial growth factor (VEGF) using commercially available biomaterials.ObjectivesTo evaluate anterior cruciate (ACLR) a VEGF functionalized suture tape in rabbit model of ACLR with semitendinosus autograft.MethodsVEGF-binding peptides were covalently bonded to polyethylene (ST) generate constructs. Forty-six female New Zealand white ACLs...