A5025619853- Acute Lymphoblastic Leukemia research
- Immune Cell Function and Interaction
- Neuropeptides and Animal Physiology
- Hematopoietic Stem Cell Transplantation
- Pancreatic function and diabetes
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Click Chemistry and Applications
- T-cell and Retrovirus Studies
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- Chemokine receptors and signaling
- Peptidase Inhibition and Analysis
- Wnt/β-catenin signaling in development and cancer
- Cancer, Stress, Anesthesia, and Immune Response
- Fibroblast Growth Factor Research
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- Single-cell and spatial transcriptomics
- Medicinal plant effects and applications
- Aldose Reductase and Taurine
Inserm
2007-2024
Institut de Radiobiologie Cellulaire et Moléculaire
2014-2024
Université Paris Cité
2009-2024
Université Paris-Saclay
2017-2024
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2014-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2014-2024
CEA Paris-Saclay
2019-2024
Unit of Functional and Adaptive Biology
2023-2024
Centre National de la Recherche Scientifique
2008-2024
La Ligue Contre le Cancer
2023
T cell acute lymphoblastic leukemia (T-ALL) is an aggressive cancer that frequently associated with activating mutations in NOTCH1 and dysregulation of MYC. Here, we performed 2 complementary screens to identify FDA-approved drugs drug-like small molecules activity against T-ALL. We developed a zebrafish system screen for toxic toward MYC-overexpressing thymocytes used human T-ALL line synergize Notch inhibitors. identified the antipsychotic drug perphenazine both due its ability induce...
PurposeUltra-high-dose-rate FLASH radiation therapy has been shown to minimize side effects of irradiation in various organs while keeping antitumor efficacy. This property, called the effect, caused enthusiasm oncology community because it opens opportunities for safe dose escalation and improved outcome. Here, we investigated impact ultra-high-dose-rate versus conventional-dose-rate (CONV) total body (TBI) on humanized models T-cell acute lymphoblastic leukemia (T-ALL) normal human...
Abstract Basic calcium phosphate (BCP) crystals, including hydroxyapatite, octacalcium (OCP) and carbonate-apatite, have been associated with severe osteoarthritis several degenerative arthropathies. Most studies considered the chondrocyte to be a bystander in pathogenesis of crystal deposition disease, assuming that synovial cell cytokines were only triggers activation. In present study we identified direct activation articular chondrocytes by OCP which are BCP crystals greatest potential...
Wnt/β-catenin signaling is critical for a variety of fundamental cellular processes. Here, we investigated the implication in vivo regulation β-cell growth and regeneration normal diabetic rats. To this aim, TCF7L2, distal effector canonical Wnt pathway, was knocked down groups rats by use specific antisense morpholino-oligonucleotides. In other rats, pathway activated inhibition its negative regulator GSK-3β. GSK-3β inactivated either LiCl or anti-GSK-3β oligonucleotides. The mass evaluated...
The cellular receptor Notch1 is a central regulator of T‐cell development, and as consequence, pathway appears upregulated in > 65% the cases acute lymphoblastic leukemia (T‐ALL). However, strategies targeting signaling render only modest results clinic due to treatment resistance severe side effects. While many investigations reported different aspects tumor cell growth progression controlled by Notch1, less known regarding modifications metabolism induced upregulation T‐ALL. Previously,...
Background Keratinocyte growth factor (KGF; palifermin) is a with high degree of specificity for epithelial cells. KGF an important effector and tissue homeostasis in various organs including the pancreas. Here we investigated intracellular signaling pathways involved mediation pancreatic ductal cell proliferation differentiation induced by exogenous during beta-cell regeneration diabetic rat. Methodology Results In vitro vivo duct was measured BrdU incorporation assay. The implication...
Hypoxia plays a major role in the physiology of hematopoietic and immune niches. Important clues from works mouse have paved way to investigate low O2 levels hematopoiesis. However, whether hypoxia impacts initial steps human lymphopoiesis remains unexplored. Here, we show that regulates cellular metabolic profiles umbilical cord blood (UCB)-derived progenitor cells. more specifically enhances vitro lymphoid differentiation potentials lymphoid-primed multipotent progenitors (LMPPs)...
Abstract Resistance to chemotherapy, a major therapeutic challenge in the treatment of T-cell acute lymphoblastic leukemia (T-ALL), can be driven by interactions between leukemic cells and microenvironment that promote survival cells. The bone marrow, an important niche, has low oxygen partial pressures highly participate regulation normal hematopoiesis. Here we show hypoxia inhibits T-ALL cell growth slowing down cycle progression, decreasing mitochondria activity, increasing glycolysis,...
Abstract Adrenomedullin (ADM) has been shown to mediate multifunctional responses in cell culture and animal system such as regulation of growth apoptosis. ADM stimulates the proliferation osteoblasts vitro promotes bone vivo. The ability influence osteoblastic number through inhibition apoptosis not yet studied. To address this question we have investigated its effect on serum‐deprived cells using mouse MC3T3‐E1 which express both receptors. Treatment with significantly blunted apoptosis,...
Abstract Rheumatoid arthritis (RA) is characterized by fibroblast-like synoviocyte (FLS) hyperplasia, which partly ascribable to decreased apoptosis. In this study, we show that adrenomedullin (ADM), an antiapoptotic peptide, constitutively secreted in larger amounts FLS from joints with RA (RA-FLS) than osteoarthritis (OA-FLS). ADM secretion was regulated TNF-α. Peptidylglycine α-amidating monooxygenase, the ADM-processing enzyme, expressed at mRNA level both RA-FLS and OA-FLS. Constituents...
Abstract Introduction Rheumatoid arthritis (RA) is characterized by bone and cartilage invasion fibroblast-like synoviocytes (FLSs). Adrenomedullin, a peptide with anabolic antiapoptotic properties, secreted rheumatoid FLSs. Adrenomedullin also increases the expression of adhesion molecules in endothelial cells keratinocytes. Here, we investigated whether adrenomedullin mediated FLS to extracellular matrix (ECM) proteins. Methods FLSs were isolated from synovial tissues RA osteoarthritis...
Abstract Objective Adrenomedullin 22–52 is a truncated peptide derived from adrenomedullin, growth factor with antiapoptotic and immunoregulatory properties. It can act as an agonist or antagonist depending on cell type. Its in vivo effects are unknown, but adrenomedullin could possess immunomodulatory This study was undertaken to evaluate the effect of mouse model arthritis. Methods DBA/1 mice collagen‐induced arthritis (CIA) were treated 1.2 μg/gm , saline at onset. Bone mineral density...
Glucocorticoids (GCs) are the main treatment for autoimmune and inflammatory disorders also used as immunosuppressive therapy patients with organ transplantation. However, these treatments have several side effects, including metabolic disorders. Indeed, cortico-therapy may induce insulin resistance, glucose intolerance, disrupted glucagon secretion, excessive gluconeogenesis, leading to diabetes in susceptible individuals. Recently, lithium has been shown alleviate deleterious effects of...