A5066691688- Cutaneous lymphoproliferative disorders research
- T-cell and Retrovirus Studies
- Lymphoma Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Chronic Myeloid Leukemia Treatments
- CAR-T cell therapy research
- Fungal Infections and Studies
- Click Chemistry and Applications
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Histone Deacetylase Inhibitors Research
- Adipokines, Inflammation, and Metabolic Diseases
- Nail Diseases and Treatments
- Toxin Mechanisms and Immunotoxins
- Effects of Radiation Exposure
- Ion Channels and Receptors
- Adipose Tissue and Metabolism
- Pancreatic and Hepatic Oncology Research
- Phytochemicals and Antioxidant Activities
- Hematological disorders and diagnostics
- Phosphodiesterase function and regulation
- Ubiquitin and proteasome pathways
Inserm
2011-2023
Université de Bordeaux
2018-2023
Bordeaux Population Health
2017-2023
Institut de Radiobiologie Cellulaire et Moléculaire
2011-2021
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2011-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2011-2021
Université Paris Cité
2011-2021
Université Paris-Saclay
2011-2021
Translational Research in Oncology
2021
Délégation Paris 7
2012-2017
T cell acute lymphoblastic leukemia (T-ALL) is an aggressive cancer that frequently associated with activating mutations in NOTCH1 and dysregulation of MYC. Here, we performed 2 complementary screens to identify FDA-approved drugs drug-like small molecules activity against T-ALL. We developed a zebrafish system screen for toxic toward MYC-overexpressing thymocytes used human T-ALL line synergize Notch inhibitors. identified the antipsychotic drug perphenazine both due its ability induce...
Normal tissue homeostasis is maintained by dynamic interactions between epithelial cells and their microenvironment. Disrupting this can induce aberrant cell proliferation, adhesion, function migration that might promote malignant behavior. Indeed, stromal-epithelial contribute to pancreatic ductal adenocarcinoma (PDAC) spread metastasis, raises the possibility novel stroma-targeted therapies represent additional approaches for combating disease. The aim of present study was determine effect...
PurposeUltra-high-dose-rate FLASH radiation therapy has been shown to minimize side effects of irradiation in various organs while keeping antitumor efficacy. This property, called the effect, caused enthusiasm oncology community because it opens opportunities for safe dose escalation and improved outcome. Here, we investigated impact ultra-high-dose-rate versus conventional-dose-rate (CONV) total body (TBI) on humanized models T-cell acute lymphoblastic leukemia (T-ALL) normal human...
White adipose tissue (WAT) is a heterogeneous tissue, found in various locations throughout the body, containing mature adipocytes and stroma-vascular fraction (SVF). The SVF includes large proportion of immune hematopoietic cells, among which, mast cells that contribute to diet-induced obesity. In this study, we asked whether present mice could derive from stem/progenitor (HSPC) identified tissue. We therefore performed both vitro vivo experiments dedicated monitoring progeny WAT-derived...
Acute megakaryoblastic leukemia (AMKL) is a heterogeneous disease generally associated with poor prognosis. Gene expression profiles indicate the existence of distinct molecular subgroups, and several genetic alterations have been characterized in past years, including t(1;22)(p13;q13) trisomy 21 GATA1 mutations. However, majority patients do not present known mutations, limited access to primary patient leukemic cells impedes efficient development novel therapeutic strategies. In this...
Adipose-derived stromal cells (ADSCs) are close relatives of bone marrow mesenchymal (BM-MSCs). The ease access to subcutaneous fat pad and the abundance precursors make tissue an attractive source for clinicians. However, their ability support hematopoietic stem in vitro vivo has not been established definitively. Thus, usefulness supporting cell engraftment is as clear with BM-MSCs. In this article, we show that human ADSCs, cultured a good manufacturing practice medium, maintain early...
Monoallelic 6p25.3 rearrangements associated with DUSP22 (Dual Specificity Phosphatase 22) gene silencing have been reported in CD30+ peripheral T-cell lymphomas (PTCL), mostly anaplastic morphology and of cutaneous origin. However, the mechanism second allele putative tumor suppressor function not investigated so far. Here, we show that presence, most individuals, an inactive paralog hampers genetic epigenetic evaluation gene. Identification DUSP22-specific single-nucleotide polymorphisms...
Abstract Resistance to chemotherapy, a major therapeutic challenge in the treatment of T-cell acute lymphoblastic leukemia (T-ALL), can be driven by interactions between leukemic cells and microenvironment that promote survival cells. The bone marrow, an important niche, has low oxygen partial pressures highly participate regulation normal hematopoiesis. Here we show hypoxia inhibits T-ALL cell growth slowing down cycle progression, decreasing mitochondria activity, increasing glycolysis,...
Abstract Sézary Syndrome (SS) is a rare aggressive epidermotropic cutaneous T-cell lymphoma (CTCL) defined by erythroderma, pruritis, and circulating atypical CD4 + clonal population. The diversity of cell (SC) phenotype genotype may reflect either plasticity or heterogeneity, which was difficult to evaluate dynamically until the achievement long-term SC expansion. Therefore, we developed six culture conditions allowing for expansion their monoclonality in four seven SS cases. Engraftment...
Cutaneous T-cell lymphomas (CTCLs) are telomerase-positive tumors expressing hTERT, although neither gene rearrangement/amplification nor promoter hotspot mutations could explain the hTERT re-expression. As is rich in CpG, we investigated contribution of epigenetic mechanisms its We analyzed methylation status CTCL cells compared with healthy cells. Gene-specific analyses revealed a common pattern exclusively tumor This encompassed hypermethylated distal region from -650 to -150 bp and...
Recent massive parallel sequencing data have evidenced the genetic diversity and complexity of Sézary syndrome mutational landscape with TP53 alterations being most prevalent abnormality. We analyzed a cohort 35 patients SS control group 8 chronic inflammatory dermatoses. status was at different clinical stages especially in 9 past-history mycosis fungoides (MF), coined secondary SS. mutations were only detected 10 either primary or (29%) corresponding to point mutations, small insertions...
Ca2+ release-activated channels, composed of Orai1 and STIM1 (stromal interaction molecule 1) proteins, are the main entry mechanism in lymphocytes. Their role cell migration metastasis is demonstrated solid cancers but it remains elusive malignant hemopathies. Diffuse large B lymphoma (DLBCL) characterized by dissemination neoplastic cells throughout organism which under control chemokines such as Stromal Derived Factor 1 (SDF-1) its receptor CXCR4. CXCR4 activation triggers a complex...
As a major cancer hallmark, there is sustained interest in understanding the telomerase contribution to carcinogenesis order therapeutically target this enzyme. This particularly relevant primary cutaneous T-cell lymphomas (CTCL), malignancy showing dysregulation with few investigative data available. In CTCL, we examined mechanisms involved transcriptional activation and activity regulation. We analyzed 94 CTCL patients from Franco-Portuguese cohort, as well 8 cell lines, comparison 101...
// Sandrine Poglio 1, 2, 3, 4 , Daniel Lewandowski 4, 5 Julien Calvo Aurélie Caye 6, 7 Audrey Gros 8, 9 Elodie Laharanne Thierry Leblanc 10 Judith Landman-Parker 11 André Baruchel Jean Soulier 12, 13 Paola Ballerini Emmanuelle Clappier Françoise Pflumio 1 Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), DSV-IRCM-SCSR-LSHL, UMR 967, Fontenay-aux-Roses, France 2 INSERM, U967, 3 Université Paris Diderot, Sorbonne Cité, Paris-Sud, CEA, DSV-IRCM-SCSR-LRTS, 6 Paris, Assistance...