Beat Bornhäuser
A5008128025- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- CAR-T cell therapy research
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Ubiquitin and proteasome pathways
- Childhood Cancer Survivors' Quality of Life
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Lymphoma Diagnosis and Treatment
- RNA modifications and cancer
- Signaling Pathways in Disease
- Cancer therapeutics and mechanisms
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Protein Degradation and Inhibitors
- CRISPR and Genetic Engineering
- Phagocytosis and Immune Regulation
- Neuroblastoma Research and Treatments
- Molecular Biology Techniques and Applications
University Children's Hospital Zurich
2016-2025
University of Zurich
2000-2022
University of Perugia
2019
University Hospital Schleswig-Holstein
2018
University of Lübeck
2018
German Center for Infection Research
2018
Kiel University
2018
Jazz Pharmaceuticals (Italy)
2018
International Breast Cancer Study Group
2018
Novartis (Switzerland)
2018
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, on this topic has continued to accelerate, and many new scientists have entered field. Our knowledge base relevant technologies also been expanding. Accordingly, it is important update these monitoring autophagy different organisms. Various reviews described range assays that used purpose. Nevertheless, there continues be confusion regarding acceptable methods measure autophagy, especially...
In vivo resistance to first-line chemotherapy, including glucocorticoids, is a strong predictor of poor outcome in children with acute lymphoblastic leukemia (ALL). Modulation cell death regulators represents an attractive strategy for subverting such drug resistance. Here we report complete resensitization multidrug-resistant childhood ALL cells glucocorticoids and other cytotoxic agents subcytotoxic concentrations obatoclax, putative antagonist BCL-2 family members. The reversal...
Purpose Somatic deletions that affect the lymphoid transcription factor–coding gene IKZF1 have previously been reported as independently associated with a poor prognosis in pediatric B-cell precursor (BCP) acute lymphoblastic leukemia (ALL). We now refined prognostic strength of by analyzing effect co-occurring deletions. Patients and Methods The analysis involved 991 patients BCP ALL treated Associazione Italiana Ematologia ed Oncologia Pediatrica–Berlin-Frankfurt-Muenster (AIEOP-BFM) 2000...
Apoptosis is a complex multi-step process driven by caspase-dependent proteolytic cleavage cascades. Dysregulation of apoptosis promotes tumorigenesis and limits the efficacy chemotherapy. To assess interactions among caspases during apoptosis, we disrupted caspase-8, -9, -3, -7, or -6 combinations thereof, using CRISPR-based genome editing in living human leukemia cells. While loss apical initiator caspase-8 -9 partially blocked extrinsic intrinsic respectively, only combined caspase-3 -7...
Abstract Dystrophin is selectively localized in the postsynaptic density of neurons cerebral cortex, hippocampus and cerebellum. Here, we show by double‐immunofluorescence staining that dystrophin extensively colocalized with GABA A receptor subunit clusters these brain regions. To determine relevance this observation, investigated mdx mice, which provide a model Duchenne muscular dystrophy, whether absence affects synaptic clustering receptors. marked reduction number immunoreactive for α1...
Cancer stem cells (CSCs) have been identified in a number of solid tumors, but not yet rhabdomyosarcoma (RMS), the most frequently occurring soft tissue tumor childhood. Hence, aim this study was to identify and characterize CSC population RMS using functional approach. We found that embryonal (eRMS) cell lines can form spheres (short rhabdospheres) medium containing defined growth factors over several passages. Using an orthotopic xenograft model, we demonstrate 100 fold less sphere result...
SMAC mimetics simultaneously activate two different cell death pathways in treatment-resistant ALL.
Significance The Notch signaling cascade is deregulated by oncogenic lesions in human cancers and has therefore become an attractive therapeutic target. Inhibitory monoclonal antibodies small-molecule γ-secretase inhibitors have been developed to target the pathway at most proximal point of cascade. Major hurdles application these prevalent dose-limiting toxicities intestine. Here we report identification preclinical validation a small molecule (CB-103) that inhibits level transcription...
DYRK1A is a serine/threonine kinase encoded on human chromosome 21 (HSA21) that has been implicated in several pathologies of Down syndrome (DS), including cognitive deficits and Alzheimer's disease. Although children with DS are predisposed to developing leukemia, especially B cell acute lymphoblastic leukemia (B-ALL), the HSA21 genes contribute malignancies remain largely undefined. Here, we report overexpressed required for B-ALL. Genetic pharmacologic inhibition decreased leukemic...
Acute megakaryoblastic leukemia (AMKL) is a heterogeneous disease generally associated with poor prognosis. Gene expression profiles indicate the existence of distinct molecular subgroups, and several genetic alterations have been characterized in past years, including t(1;22)(p13;q13) trisomy 21 GATA1 mutations. However, majority patients do not present known mutations, limited access to primary patient leukemic cells impedes efficient development novel therapeutic strategies. In this...
Predictive biomarkers are required to identify patients who may benefit from the use of BH3 mimetics such as ABT-263. This study investigated efficacy ABT-263 against a panel patient-derived pediatric acute lymphoblastic leukemia (ALL) xenografts and utilized cell molecular approaches that predict in vivo sensitivity.The was tested 31 ALL composed MLL-, BCP-, T-ALL subtypes. Basal gene expression profiles were analyzed confirmed by quantitative RT-PCR, protein profiling. An vitro coculture...
Abstract Purpose: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive disease, affecting children and adults. Chemotherapy treatments show high response rates but have debilitating effects carry risk of relapse. Previous work implicated NOTCH1 other oncogenes. However, direct inhibition these pathways affects healthy tissues cancer alike. Our goal in this has been to identify enzymes active T-ALL whose activity could be targeted for therapeutic purposes. Experimental Design: To...
The LDLR is a critical factor in the regulation of blood cholesterol levels that are altered different human diseases. level cell regulated by both transcriptional and post-transcriptional events. E3 ubiquitin ligase, myosin regulatory light chain-interacting protein (Mylip)/inducible degrader LDL-R (Idol) was shown to induce degradation via ubiquitination. We have here studied novel factors mechanisms may regulate Mylip/Idol hepatocyte cells mouse macrophages. observed FGF21 present serum...