Stéphanie Sungalee
A5026045059- Cancer Genomics and Diagnostics
- Lymphoma Diagnosis and Treatment
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Bioinformatics and Genomic Networks
- Evolution and Genetic Dynamics
- Chronic Lymphocytic Leukemia Research
- DNA Repair Mechanisms
- Acute Lymphoblastic Leukemia research
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Mycobacterium research and diagnosis
- Cancer-related gene regulation
- Genomics and Rare Diseases
- Genetic factors in colorectal cancer
- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- Gene expression and cancer classification
- CAR-T cell therapy research
- Nutrition, Genetics, and Disease
- T-cell and B-cell Immunology
European Molecular Biology Laboratory
2015-2023
European Bioinformatics Institute
2022
École Polytechnique Fédérale de Lausanne
2017-2021
Swiss Cancer Center Léman
2020-2021
European Molecular Biology Organization
2017-2019
Inserm
2010-2015
Centre National de la Recherche Scientifique
2011-2015
Centre d’Immunologie de Marseille-Luminy
2010-2015
Aix-Marseille Université
2014
Centre Hospitalier Universitaire de Tours
2014
Abstract Burkitt lymphoma (BL) is the most common B-cell in children. Within International Cancer Genome Consortium (ICGC), we performed whole genome and transcriptome sequencing of 39 sporadic BL. Here, unravel interaction structural, mutational, transcriptional changes, which contribute to MYC oncogene dysregulation together with pathognomonic IG- translocation. Moreover, by mapping IGH translocation breakpoints, provide evidence that precursor at least a subset BL poised express IGHA. We...
Purpose The (14;18) translocation constitutes both a genetic hallmark and critical early event in the natural history of follicular lymphoma (FL). However, t(14;18) is also detectable blood otherwise healthy persons, its relationship with progression to disease remains unclear. Here we sought determine whether t(14;18)-positive cells individuals represent tumor precursors their detection could be used as an predictor for FL. Participants Methods Among 520,000 participants enrolled onto EPIC...
The circadian clock and the cell cycle are major cellular systems that organize global physiology in temporal fashion. It seems conceivable potentially conflicting programs coordinated. We show here overexpression of MYC U2OS cells attenuates conversely promotes proliferation while downregulation strengthens reduces proliferation. Inhibition is crucially dependent on formation repressive complexes with MIZ1 subsequent core genes BMAL1 (ARNTL), CLOCK NPAS2. furthermore expression levels...
It has recently been demonstrated that memory B cells can reenter and reengage germinal center (GC) reactions, opening the possibility multi-hit lymphomagenesis gradually occurs throughout life during successive immunological challenges. Here, we investigated this scenario in follicular lymphoma (FL), an indolent GC-derived malignancy. We developed a mouse model recapitulates FL hallmark t(14;18) translocation, which results constitutive activation of antiapoptotic protein cell 2 (BCL2)...
Abstract Chromatin compartmentalization reflects biological activity. However, inference of chromatin sub-compartments and compartment domains from chromosome conformation capture (Hi-C) experiments is limited by data resolution. As a result, these have been characterized only in few cell types systematic comparisons across multiple tissues conditions are missing. Here, we present Calder, an algorithmic approach that enables the identification multi-scale at variable Calder allows to infer...
B cells have the unique property to somatically alter their immunoglobulin (IG) genes by V(D)J recombination, somatic hypermutation (SHM) and class-switch recombination (CSR). Aberrant targeting of these mechanisms is implicated in lymphomagenesis, but mutational processes are poorly understood. By performing whole genome transcriptome sequencing 181 germinal center derived B-cell lymphomas (gcBCL) we identified distinct signatures linked SHM CSR. We show that not only SHM, presumably also...
Follicular lymphoma (FL) is an incurable form of B cell lymphoma. Genomic studies have cataloged common genetic lesions in FL such as translocation t(14;18), frequent losses chromosome 6q, and mutations epigenetic regulators EZH2 Using a focused screen, we identified SESTRIN1 relevant target the 6q deletion demonstrate tumor suppression by vivo. Moreover, direct lymphoma-specific gain-of-function mutation (EZH2Y641X ). inactivation disrupts p53-mediated control mammalian rapamycin complex 1...
MicroRNA are well-established players in post-transcriptional gene regulation. However, information on the effects of microRNA deregulation mainly relies bioinformatic prediction potential targets, whereas proof direct physical microRNA/target messenger RNA interaction is mostly lacking. Within International Cancer Genome Consortium Project "Determining Molecular Mechanisms Malignant Lymphoma by Sequencing", we performed miRnome sequencing from 16 Burkitt lymphomas, 19 diffuse large B-cell...
Abstract Double-strand breaks (DSBs) are the most toxic type of DNA lesions. Cells repair these lesions using either end protection- or resection-coupled mechanisms. To study DSB choice, we present C olor A ssay T racing- R epair (CAT-R) to simultaneously quantify via protection and resection pathways. CAT-R introduces DSBs CRISPR/Cas9 in a tandem fluorescent reporter, whose distinguishes small insertions/deletions from large deletions. We demonstrate applications chemical genetic screens....
The presentation of the Working Formulation for non-Hodgkin lymphomas by C. Berard was highlight 1-ICML, although at that time Hodgkin lymphomas, cell origin still unknown and even it could not yet be completely excluded, a neoplastic disease.Remembering one realizes huge evolution our lymphoma understanding has meanwhile taken place.ICML hereby played pivotal role only as platform exchanging knowledge but also an event catalyzing initiation collective efforts, which have led to some most...
Histone methylation-modifiers, such as EZH2 and KMT2D, are recurrently altered in B-cell lymphomas. To comprehensively describe the landscape of alterations affecting genes encoding histone methylation-modifiers lymphomagenesis we investigated whole genome transcriptome data 186 mature lymphomas sequenced ICGC MMML-Seq project. Besides confirming common KMT2D (47% cases), (17%), SETD1B (5%), PRDM9 (4%), KMT2C SETD2 also identified by prior exome or RNA-sequencing studies, here found...
Abstract Recent developments in sequencing technologies lead to the discovery of a novel form genome instability, termed chromothripsis. This catastrophic genomic event, involved cancer formation, is characterized by tens hundreds locally clustered rearrangements on one chromosome, acquired simultaneously. We hypothesized that leukemias developing individuals with Ataxia Telangiectasia, who are born two mutated copies ATM gene, essential guardian stability, would show higher prevalence for...