A5000895354- Hematopoietic Stem Cell Transplantation
- Zebrafish Biomedical Research Applications
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Orthopedic Surgery and Rehabilitation
- Immune cells in cancer
- Congenital Diaphragmatic Hernia Studies
- Neonatal Respiratory Health Research
- Cardiac Arrhythmias and Treatments
- Neurotransmitter Receptor Influence on Behavior
- Pleural and Pulmonary Diseases
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Acute Myeloid Leukemia Research
- Angiogenesis and VEGF in Cancer
- Elbow and Forearm Trauma Treatment
- Historical Studies and Socio-cultural Analysis
- Intestinal Malrotation and Obstruction Disorders
- Immune Cell Function and Interaction
- Neuroscience and Neuropharmacology Research
- Congenital Anomalies and Fetal Surgery
- Receptor Mechanisms and Signaling
- Biomedical Ethics and Regulation
- Radiation Dose and Imaging
- Ultrasound in Clinical Applications
Laboratoire de Biologie du Développement
2010-2024
Sorbonne Université
2012-2024
Sorbonne Paris Cité
2024
Institut de Biologie Paris-Seine
2015-2021
Royal Victoria Hospital
2021
University of Ulster
2021
Centre National de la Recherche Scientifique
2002-2020
Assistance Publique – Hôpitaux de Paris
2020
Inserm
2008-2018
Centre Hospitalier Universitaire de Grenoble
1997-2017
The molecular signals driving tendon development are not fully identified. We have undertaken a transcriptome analysis of mouse limb cells that were isolated at different stages based on scleraxis (Scx) expression. Microarray comparisons allowed us to establish list genes regulated in during development. Bioinformatics the showed two most strongly modified signalling pathways TGF-β and MAPK. TGF-β/SMAD2/3 gain- loss-of-function experiments explants mesenchymal stem was sufficient required...
Abstract Objectives To investigate the correlation between fetal lung volume (FLV), measured with magnetic resonance imaging (MRI), and postnatal mortality in newborns prenatally diagnosed isolated congenital diaphragmatic hernia (CDH). Methods In a 4‐year prospective multicenter study, 77 fetuses CDH 20 33 weeks' gestation underwent fast spin‐echo T2‐weighted MRI. These MRI‐FLV measurements were compared previously published normative curve obtained 215 without thoracoabdominal...
Hematopoietic stem cell (HSC) self-renewal and differentiation is regulated by cellular molecular interactions with the surrounding microenvironment. During ontogeny, aorta–gonad–mesonephros (AGM) region autonomously generates first HSCs serves as HSC-supportive Because identity of AGM microenvironment yet unclear, we examined two closely related stromal clones that differentially support HSCs. Expression analyses identified three putative HSC regulatory factors, β-NGF (a neurotrophic...
ABSTRACT The first hematopoietic stem and progenitor cells (HSPCs) emerge in the Aorta-Gonad-Mesonephros (AGM) region of mid-gestation mouse embryo. However, precise nature their supportive mesenchymal microenvironment remains largely unexplored. Here, we profiled transcriptomes laser micro-dissected aortic tissues at three developmental stages individual AGM cells. Computational analyses allowed identification several cell subpopulations within E11.5 mesenchyme, with presence a yet...
Extracellular vesicles (EVs) have been recently reported as crucial mediators in cell-to-cell communication development and disease. In this study, we investigate whether mesenchymal stromal cells that constitute a supportive microenvironment for hematopoietic stem progenitor (HSPCs) released EVs could affect the gene expression function of HSPCs. By taking advantage two fetal liver-derived lines with widely differing abilities to maintain HSPCs ex vivo, demonstrate play critical role...
While enucleation is a critical step in the terminal differentiation of human red blood cells, molecular mechanisms underlying this unique process remain unclear. To investigate erythroblast enucleation, we studied erythroid embryonic stem cells (hESCs), which provide model for deeper understanding development and multiple cell types. First, using two-step protocol, demonstrated that from hESCs directly dependent on age embryoid bodies. Second, by choosing two extreme conditions culture,...
Abstract Background The death rate due to suicide in elderly people is particularly high. As part of selective prevention measures for at-risk populations, the WHO recommends training “gatekeepers”. Methods In order assess impact gatekeeper members staff, we carried out a controlled quasi-experimental study over course one year, comparing 12 nursing homes where at least 30% staff had undergone with without trained staff. We collected data about residents considered be suicidal, their...
The cardinal property of bone marrow (BM) stromal cells is their capacity to contribute hematopoietic stem cell (HSC) niches by providing mediators assisting HSC functions. In this study we first contrasted transcriptomes at different developmental stages and then included large number HSC-supportive non-supportive samples. Application a combination algorithms, comprising one identifying reliable paths potential causative relationships in complex systems, revealed gene networks...
CD105 is an auxiliary receptor for the transforming growth factor beta superfamily, highly expressed on proliferating endothelial cells and adult hematopoietic stem cells. Because mRNA expression was reported in developing aortic region, we further characterized its profile aorta examined potential of CD105+ Aortic cells, intra-aortic cell clusters purified fraction enriched progenitor/hematopoietic activity CD105. short-term clonogenic progenitors were CD105intermediate population whereas...
AFTER neonatal lesions of dopamine neurones, an enhanced behavioural responsiveness towards D1 agonists has been described, suggesting a receptor hypersensitivity. In the present study, unilateral striatal denervation in newborn rats induced pronounced rotational behaviour following apomorphine injection at adult age, without any change density binding sites denervated striatum. The amount stimulatory Golfα subunit was increased by 35% lesioned large form and short forms Gsα were also 26%...
Holoprosencephaly (HPE) is the most common forebrain defect in humans. It results from incomplete midline cleavage of prosencephalon and can be caused by environmental genetic factors. HPE usually described as a continuum brain malformations severe alobar to middle interhemispheric fusion variant or syntelencephaly. A microform limited craniofacial features such congenital nasal pyriform aperture stenosis single central maxillary incisor, without malformation. Among heterogeneous causes HPE,...
We have reported previously the presence of two T cell receptor beta-chain constant region (Cbeta) isotypes in Mexican axolotl. Specific Dbeta and Jbeta segments were present at Vbeta-Cbeta1 Vbeta-Cbeta2 junctions nine Vbeta families which associate with both characterized. This report describes new Cbeta isotypes, Cbeta3 Cbeta4. About 70 % amino acids are identical to Cbeta1 Cbeta2. A Dbeta3 a single Jbeta3 found Vbeta-Cbeta3 junctions. The consensus core sequence (TACGTGGCTACGTGGG) differs...
The conventional approach for MRI procedures in very young children is to use general anesthesia which comes with inherent risks. Non-pharmacological strategies reduce anxiety have also been described, but they all require patient cooperation. purpose of the study was evaluate ability complete diagnosis using temporary spica cast immobilization (TSCI) less than 3 years old undergoing lower limb disorders.A retrospective review identified 14 under that had required an a disorder, TSCI....