A5086754571- Adipose Tissue and Metabolism
- Tendon Structure and Treatment
- Congenital heart defects research
- Adipokines, Inflammation, and Metabolic Diseases
- Exercise and Physiological Responses
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Genomics and Chromatin Dynamics
- Muscle Physiology and Disorders
- Mesenchymal stem cell research
- Orthopedic Surgery and Rehabilitation
- Knee injuries and reconstruction techniques
- Neurobiology and Insect Physiology Research
- Pluripotent Stem Cells Research
- Retinoids in leukemia and cellular processes
- Adrenal Hormones and Disorders
- Estrogen and related hormone effects
- Thyroid Disorders and Treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hormonal Regulation and Hypertension
- Single-cell and spatial transcriptomics
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Periodontal Regeneration and Treatments
- Reconstructive Surgery and Microvascular Techniques
Laboratoire de Biologie du Développement
2008-2024
Sorbonne Université
2008-2023
Centre National de la Recherche Scientifique
2008-2023
Inserm
2014-2023
Institut de Biologie Paris-Seine
2016-2023
Sorbonne Paris Cité
2020
University of Pittsburgh
2018
Physiologie et Médecine Expérimentale du Coeur et des Muscles
2017
Evolution des Régulations Endocriniennes
2002-2011
Laboratoire d’Ethologie Expérimentale et Comparée
2002
Tendon formation and repair rely on specific combinations of transcription factors, growth mechanical parameters that regulate the production spatial organization type I collagen. Here, we investigated function zinc finger factor EGR1 in tendon formation, healing, using rodent animal models mesenchymal stem cells (MSCs). Adult tendons Egr1-/- mice displayed a deficiency expression genes, including Scx, Col1a1, Col1a2, were mechanically weaker compared with their WT littermates. was recruited...
The molecules involved in vertebrate tendon formation during development remain largely unknown. To date, only two DNA-binding proteins have been identified as being formation, the basic helix-loop-helix transcription factor Scleraxis and, recently, Mohawk homeobox gene. We investigated involvement of early growth response factors Egr1 and Egr2 formation. established that expression cells was correlated with increase collagen cell differentiation embryonic limbs. Vertebrate relies on a...
The molecular signals driving tendon development are not fully identified. We have undertaken a transcriptome analysis of mouse limb cells that were isolated at different stages based on scleraxis (Scx) expression. Microarray comparisons allowed us to establish list genes regulated in during development. Bioinformatics the showed two most strongly modified signalling pathways TGF-β and MAPK. TGF-β/SMAD2/3 gain- loss-of-function experiments explants mesenchymal stem was sufficient required...
The molecular programme underlying tendon development has not been fully identified. Interactions with components of the musculoskeletal system are important for limb formation. Limb tendons initiate their independently muscles; however, muscles required further differentiation. We show that both FGF/ERK MAPK and TGFβ/SMAD2/3 signalling pathways sufficient SCX expression in chick undifferentiated cells, whereas pathway inhibits Scx mouse mesodermal cells. During differentiation, muscle...
In higher vertebrates, the primordium of nervous system, neural tube, is shaped along rostrocaudal axis through two consecutive, radically different processes referred to as primary and secondary neurulation. Failures in neurulation lead severe anomalies called tube defects (NTDs), which are among most common congenital malformations humans. Mechanisms causing NTDs humans remain ill-defined. Of particular interest, thoracolumbar region, encompasses many NTD cases spine, corresponds junction...
Vascularized composite allotransplantation opens new possibilities in reconstructive transplantation such as hand or face transplants. Lifelong immunosuppression and its side-effects are the main drawbacks of this procedure. Mesenchymal stem cells (MSCs) have clinically useful immunomodulatory effects may be able to reduce burden chronic immunosuppression. Herein, we assess compare characteristics capacities bone marrow- adipose tissue-derived MSCs isolated from same human individual across...
Thyroid hormone receptors (TR) act as activators of transcription in the presence thyroid (T(3)) and repressors its absence. While many vitro approaches have been used to study molecular mechanisms TR action, their physiological relevance has not addressed. Here we investigate how regulates gene expression during vertebrate postembryonic development by using T(3)-dependent amphibian metamorphosis a model. Earlier studies suggest that acts repressor premetamorphosis when T(3) is absent. We...
The diversity of thyroid hormone T3 effects in vivo makes their molecular analysis particularly challenging. Indeed, the current model action and its receptors on transcription does not reflect this diversity. Here, T3-dependent amphibian metamorphosis was exploited to investigate, an developmental context, how directly regulates gene expression. Two, direct positively regulated T3-response genes encoding factors were analyzed: receptor β (TRβ) TH/bZIP. Reverse transcription-real-time...
The LIM homeodomain gene Islet-1 (ISL1) encodes a transcription factor that has been associated with the multipotency of human cardiac progenitors, and in mice enables correct deployment second heart field (SHF) cells to become myocardium atria, right ventricle outflow tract. Other markers have identified characterize subdomains SHF, such as fibroblast growth Fgf10 its anterior region. While functional evidence essential contribution demonstrated many vertebrate species, SHF expression Isl1...
ABSTRACT Introduction : Peripheral nerve damage is associated with high long‐term morbidity. Because of beneficial secretome, immunomodulatory effects, and ease clinical translation, transplantation adipose‐derived stem cells (ASC) represents a promising therapeutic modality. Methods Effect ASC delivery in poloxamer hydrogel was assessed rat sciatic model critical‐sized (1.5 cm) peripheral injury. Nerve/muscle unit regeneration via immunostaining explanted nerve, quantitative polymerase...
ABSTRACT The location and regulation of fusion events within skeletal muscles during development remain unknown. Using the marker myomaker (Mymk), named TMEM8C in chicken, as a readout fusion, we identified co-segregation TMEM8C-positive cells MYOG-positive single-cell RNA-sequencing datasets limbs from chicken embryos. We found that transcripts, MYOG transcripts fusion-competent were preferentially regionalized central regions foetal muscles. also similar regionalization for gene encoding...
The basic helix-loop-helix transcription factor MyoD is a central actor that triggers the skeletal myogenic program. Cell-autonomous and non-cell-autonomous regulatory pathways must tightly control expression to ensure correct initiation of muscle program at different places in embryo developmental times. In present study, we have addressed involvement Sim2 (single-minded 2) limb embryonic myogenesis. bHLH-PAS inhibits by active repression displays enhanced ventral masses during chick mouse...
The morphogenesis of the vertebrate hindbrain involves generation metameric units called rhombomeres (r), and Krox20 encodes a transcription factor that is expressed in r3 r5 plays major role this segmentation process. Our knowledge basis Krox20regulation rather confusing, especially concerning involvement Hox factors. To investigate issue, we studied one Krox20hindbrain cis-regulatory sequences, element C, which active r3-r5 only initiator r3. We show C contains multiple binding sites for...
Abstract In mice, exercise, cold exposure and fasting lead to the differentiation of inducible-brown adipocytes, called beige within white adipose tissue have beneficial effects on fat burning metabolism, through heat production. This browning process is associated with an increased expression key thermogenic mitochondrial uncoupling protein 1, Ucp1. Egr1 transcription factor has been described as a regulator programs, depletion spontaneous increase subcutaneous browning, in absence external...
Beige adipocyte differentiation within white adipose tissue, referred to as browning, is seen a possible mechanism for increasing energy expenditure. The molecular regulation underlying the thermogenic browning process has not been entirely elucidated. Here, we identify zinc finger transcription factor EGR1 negative regulator of beige fat program. Loss Egr1 in mice promotes absence external stimulation and leads an increase Ucp1 expression, which encodes key mitochondrial uncoupling...
In humans, glucocorticoids (GCs) are commonly prescribed because of their anti-inflammatory and immunosuppressive properties. However, high doses GCs often lead to side effects, including diabetes lipodystrophy. We recently reported that adipocyte glucocorticoid receptor (GR)–deficient (AdipoGR-KO) mice under corticosterone (CORT) treatment exhibited a massive adipose tissue (AT) expansion associated with paradoxical improvement metabolic health compared control mice. whether GR may...
Abstract Tendons are composed of type I collagen fibrils and specialized extracellular matrix (ECM) proteins that hierarchically organized to transmit forces from muscle bone. Reliable affordable models human tendon constructs requested serve surgical treatment after injury in vitro pharmacological testing. Here, using adipose stromal cells embedded a 3D type-I submitted static uniaxial geometrical constraint, we generated assessed their mechanical properties gene expression for up 21 days...
Early patterning of the vertebrate neural plate involves a complex hierarchy inductive interactions orchestrated by signalling molecules and their antagonists. The morphogen retinoic acid, together with Cyp26 enzymes which degrade it, play central role in this process. cyp26a1 gene expressed anterior thus contributes to fine modulation rostrocaudal acid gradient. Despite important early brain formation, mechanisms that control its expression are totally unknown. Here, we present isolation...