A5036793450- Thyroid Disorders and Treatments
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Retinoids in leukemia and cellular processes
- Epigenetics and DNA Methylation
- Physiological and biochemical adaptations
- Estrogen and related hormone effects
- Animal Genetics and Reproduction
- Neurobiology and Insect Physiology Research
- Birth, Development, and Health
- Hormonal Regulation and Hypertension
- RNA Research and Splicing
- Histone Deacetylase Inhibitors Research
- Virus-based gene therapy research
- Growth Hormone and Insulin-like Growth Factors
- Amphibian and Reptile Biology
- Cell death mechanisms and regulation
- Chromosomal and Genetic Variations
- Bone Metabolism and Diseases
- Genomics and Phylogenetic Studies
- Aquaculture Nutrition and Growth
- RNA modifications and cancer
- dental development and anomalies
- interferon and immune responses
- Effects and risks of endocrine disrupting chemicals
Centre National de la Recherche Scientifique
2014-2024
Centre National pour la Recherche Scientifique et Technique (CNRST)
2024
Digital Research Alliance of Canada
2024
Éco-Anthropologie
2018-2024
Sorbonne Université
2016-2024
Muséum national d'Histoire naturelle
2014-2022
University of Michigan
2016-2020
Evolution des Régulations Endocriniennes
2007-2018
Musée National d'Histoire Naturelle
1997-2015
Structure et Instabilité des Génomes
2000-2006
Abstract Frogs are an ecologically diverse and phylogenetically ancient group of anuran amphibians that include important vertebrate cell developmental model systems, notably the genus Xenopus . Here we report a high-quality reference genome sequence for western clawed frog, tropicalis , along with draft chromosome-scale sequences three distantly related emerging frog species, Eleutherodactylus coqui Engystomops pustulosus Hymenochirus boettgeri Frog chromosomes have remained remarkably...
Nuclear receptors (NRs) are transcription factors that implicated in several biological processes such as embryonic development, homeostasis, and metabolic diseases. To study the role of NRs it is critically important to know when where individual genes expressed. Although systematic expression studies using reverse transcriptase PCR and/or DNA microarrays have been performed classical model systems Drosophila mouse, no atlas describing NR involvement during development on a global scale has...
Amphibian metamorphosis is marked by dramatic, thyroid hormone (TH)-induced changes involving gene regulation TH receptor (TR). It has been postulated that TR-mediated involves chromatin remodeling. In the absence of ligand, TR can repress expression recruiting a histone deacetylase complex, whereas liganded recruits acetylase complex for activation. Earlier studies have led us to propose dual function model during development. premetamorphic tadpoles, unliganded represses transcription...
There is increasing evidence that the thyroid hormone (TH) receptors (THRs) can play a role in aging, cancer and degenerative diseases. In this paper, we demonstrate binding of TH T3 (triiodothyronine) to THRB induces senescence deoxyribonucleic acid (DNA) damage cultured cells tissues young hyperthyroid mice. rapid activation ATM (ataxia telangiectasia mutated)/PRKAA (adenosine monophosphate–activated protein kinase) signal transduction recruitment NRF1 (nuclear respiratory factor 1)...
Cooperative, synergistic gene regulation by nuclear hormone receptors can increase sensitivity and amplify cellular responses to hormones. We investigated thyroid (TH) glucocorticoid (GC) synergy on the Krüppel-like factor 9 (Klf9) gene, which codes for a zinc finger transcription involved in development homeostasis of diverse tissues. identified regions Xenopus mouse Klf9 genes 5-6 kb upstream start sites that supported transactivation TH plus GC. Within these regions, we found an...
N-CoR (nuclear receptor corepressor) is a corepressor for multiple transcription factors including unliganded thyroid hormone receptors (TRs). In vitro, can interact with the Sin3 corepressor, which in turn binds to histone deacetylase Rpd3 (HDAC1), predicting existence of complex containing N-CoR, Sin3, and deacetylase. However, previous biochemical studies endogenous complexes have failed find an association. Xenopus laevis eggs oocytes contain all necessary components transcriptional...
Thyroid hormone receptors (TR) act as activators of transcription in the presence thyroid (T(3)) and repressors its absence. While many vitro approaches have been used to study molecular mechanisms TR action, their physiological relevance has not addressed. Here we investigate how regulates gene expression during vertebrate postembryonic development by using T(3)-dependent amphibian metamorphosis a model. Earlier studies suggest that acts repressor premetamorphosis when T(3) is absent. We...
The main limitation of non-viral gene transfer methods is their relatively low efficiency in vivo. However, a number approaches can be taken to improve performances, whether the aim studying function during development or employing these techniques for therapy. Three delivery systems that we have been particularly involved developing are described: cationic lipid, dioctadecylamidoglycylspermine (DOGS), polymer polyethylenimine (PEI) and free DNA. application each different vivo situations...
The diversity of thyroid hormone T3 effects in vivo makes their molecular analysis particularly challenging. Indeed, the current model action and its receptors on transcription does not reflect this diversity. Here, T3-dependent amphibian metamorphosis was exploited to investigate, an developmental context, how directly regulates gene expression. Two, direct positively regulated T3-response genes encoding factors were analyzed: receptor β (TRβ) TH/bZIP. Reverse transcription-real-time...
Thyroid hormone (T(3)) induces gene regulation programs necessary for tadpole metamorphosis. Among the earliest responses to T(3) are up-regulation of receptor beta (TRbeta; autoinduction) and BTEB1 (basic transcription element-binding protein 1). is a member Krüppel family factors that bind GC-rich regions in promoters. The proximal promoter Xenopus laevis TrbetaA has seven sequences, which led us hypothesize binds regulates TrbetaA. In tadpoles frog fibroblast-derived cell line XTC-2,...
Metamorphosis in amphibians is marked by dramatic thyroid hormone-induced changes that include tail regression. To examine hormone effects on gene transcription during the early stages of resorption, we injected exogenous genes directly into caudal skeletal muscle Xenopus tadpoles and followed their expression vivo. Gene was both strong reproducible, it correlated with amount DNA injected. Moreover, continued as long animals were blocked prometamorphosis antithyroid drugs (for up to 4...
In vitro and in vivo data have demonstrated that virus-mediated p53 gene transfer can induce active cell death lung tumor regression. contrast, the therapeutic potential of bax, another apoptosis-inducing gene, has not been described. We compared bax cytotoxic effects by transient transfection an average 25 ± 5% H-322 H-358 bronchioloalveolar carcinoma lines vitro. Under these conditions, expression killed 70 to 90% transfected cells whereas only 40% them. The killing activity both genes...