A5051666296- Mesenchymal stem cell research
- Body Contouring and Surgery
- Breast Implant and Reconstruction
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Wound Healing and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Dermatologic Treatments and Research
- Biomedical Ethics and Regulation
- Facial Rejuvenation and Surgery Techniques
- Lymphatic System and Diseases
- Cancer Cells and Metastasis
- Adipose Tissue and Metabolism
- Organ and Tissue Transplantation Research
- Obesity and Health Practices
- Venous Thromboembolism Diagnosis and Management
- Surgical Sutures and Adhesives
- Body Image and Dysmorphia Studies
- Digital Imaging in Medicine
- Muscle Physiology and Disorders
- Bone fractures and treatments
- Pluripotent Stem Cells Research
- Cleft Lip and Palate Research
- Effects of Radiation Exposure
- Diabetic Foot Ulcer Assessment and Management
University of Pittsburgh
2015-2024
McGowan Institute for Regenerative Medicine
2015-2024
University of Pittsburgh Medical Center
2014-2024
Creative Commons
2018-2022
Center for Genomic Science
2020
University of Michigan
2011-2019
Miami Transplant Institute
2019
New York University
2019
Musculoskeletal Transplant Foundation
2019
Icahn School of Medicine at Mount Sinai
2019
Abstract The in vivo progenitor of culture‐expanded mesenchymal‐like adipose‐derived stem cells (ADSC) remains elusive, owing part to the complex organization stromal surrounding small vessels, and rapidity with which adipose vascular adopt a mesenchymal phenotype vitro. Immunohistostaining intact tissue was used identify three markers (CD31, CD34, CD146), together unambiguously discriminate histologically distinct inner outer rings vessel‐associated cells, as well capillary vessel...
We previously demonstrated that human pericytes, which encircle capillaries and microvessels, give rise in culture to genuine mesenchymal stem cells (MSCs). This raised the question as whether all MSC are derived from pericytes. Pericytes other defined on differential expression of CD34, CD31, CD146 were sorted stromal vascular fraction white adipose tissue. Besides CD34+ CD31- CD146- CD45- cells, reside outmost layer blood vessels, tunica adventitia, natively expressed markers gave...
Adipose tissue has been shown to contain adult mesenchymal stem cells that have therapeutic applications in regenerative medicine. There is evidence the ability of adipose precursor grow and differentiate varies among fat depots changes with age. Defining these variations cell function molecular mechanisms adipogenesis will facilitate development cell-based therapies. We compared harvested from 5 different subcutaneous (SC) 12 female patients classified into 3 age ranges (25-30, 40-45, 55-60...
Abstract The stromal‐vascular fraction (SVF) of adipose tissue is a rich source multipotent stem cells. We and others have described three major populations stem/progenitor cells in this fraction, all closely associated with small blood vessels: endothelial progenitor (EPC, CD45−/CD31+/CD34+), pericytes (CD45−/CD31−/CD146+), supra‐adventitial stromal (SA‐ASC, CD45−/CD31−/CD146−/CD34+). EPC are luminal, adventitial, SA‐ASC surround the vessel like sheath. multipotency compartments strikingly...
Abstract Objective: Because post‐bariatric surgery patients undergo massive weight loss, the resulting skin excess can lead to both functional problems and profound dissatisfaction with appearance. Correcting could improve all these corollaries, including body image. Presently, few data are available documenting image weight‐related quality of life in this population. Research Methods Procedures: Eighteen who underwent bariatric contouring completed our study. Both established surveys new...
Adipose-derived stem cells (ASCs) have been proposed to stabilize autologous fat grafts for regenerative therapy, but their safety is unknown in the setting of reconstructive surgery after mastectomy. Both bone marrow mesenchymal (MSCs) and ASC shown enhance tumorigenesis established breast cancer cell lines, primary patient material has not tested. Here, we ask whether promote vitro growth vivo metastatic clinical isolates. Metastatic pleural effusion (MPE) were used coculture experiments....
Background: Venous thromboembolism is a major patient safety issue. The Plastic Surgery Foundation–sponsored Thromboembolism Prevention Study examined whether postoperative enoxaparin prevents symptomatic venous in adult plastic surgery patients. Methods: In 2009, four sites uniformly adopted clinical protocol. Patients with Caprini score of 3 or higher received prophylaxis for the duration inpatient stay. historical control patients had an operation between 2006 and 2008 but no...
Background: Variability in harvest and processing technique may impact the success of fat grafting. This study compared properties grafts produced by differing methods assessed volume retention grafted tissue a nude mouse model. Methods: In phase I, was harvested either suction-assisted lipoaspiration or ultrasound-assisted then filtered using two different pore sizes. Graft material analyzed for average parcel size; relative oil, fat, aqueous fractions; stromal vascular fraction yield....
To analyze the impact of body mass indices on postbariatric reconstructive surgery complications.An increasing number patients are presenting after massive weight loss due to bariatric or diet and exercise. Many these have residual obesity, which may compromise outcomes.449 were enrolled in a prospective registry over 6 years. Measures included medical complications comorbidities. All cases analyzed together as well two subgroups: single procedure (Group I) multiple II).449 (407 female, 42...
To test the hypothesis that transplantation of adipose precursor cells (APCs) improves nerve regeneration and functional recovery, human APCs were transplanted into lumen a guide in 6-mm unilateral sciatic defect athymic rats. The three control groups for study biodegradable, polycaprolactone-based conduit without APCs, autograft, empty defect. Behavioral tests performed every 3 weeks, index (SFI) was calculated based on measurements from hindlimb prints. After 12 as well right left...
Adipose stem cells represent a heterogenous population. Understanding the functional characteristics of subpopulations will be useful in developing adipose cell-based therapies for regenerative medicine applications. The aim this study was to define distinct populations within stromal vascular fraction based on surface marker expression, and evaluate ability each cell type differentiate mature adipocytes.Subcutaneous whole tissue obtained by abdominoplasty from human patients. separated four...
: One issue in the adoption of autologous fat transfer to breast is concern over mammographic changes that may obscure cancer detection. The authors compared following grafting with seen after reduction.: Twenty-seven women who had normal preoperative mammograms were treated breast, including admixing adipose stem cells graft, for cosmetic augmentation. Repeated performed 12 months surgery. As a control group, postsurgical from 23 reduction mammaplasty patients compared. Eight academic...
Background: Adipose tissue reaches cellular stasis after puberty, leaving adipocytes unable to significantly expand or renew under normal physiologic conditions. This is problematic in progressive lipodystrophies, instances of scarring, and soft-tissue damage resulting from lumpectomy traumatic deformities, because adipose will not self-renew once damaged. yields significant clinical necessity for an off-the-shelf de novo replacement mechanism. Methods: A process comprising separate steps...
Bone replacement is often necessary during reconstruction of craniofacial anomalies or trauma. Adipose-derived stem cells (ASCs) possess osteogenic potential and are a promising cell source for bone tissue engineering. The present study was designed to assess the utility using ASCs regenerate in rabbit calvarial defect model.Rabbit were seeded on gelatin foam (GF) scaffolds induced medium containing morphogenetic protein (BMP)-2. Thirty-four 8-mm defects randomly treated with autograft, no...
Various biodegradable hydrogels have been employed as injectable scaffolds for tissue engineering and drug delivery. We report a double-crosslinking strategy of biocompatible derived from aminated oxidized hyaluronic acid (HA) with genipin (GP), compound naturally the gardenia fruit. Fast gelation is attributed to Schiff-base reaction between amino aldehyde groups polysaccharide derivatives, subsequent crosslinking GP results in ideal biodegradability mechanical properties. The time,...
Background: Fat grafting is a promising technique for soft-tissue augmentation, although graft retention highly unpredictable and factors that affect survival have not been well defined. Because of their capacity differentiation growth factor release, adipose-derived stem cells may key role in healing. The authors' objective was to determine whether biological properties present within human fat would correlate with vivo outcomes volume retention. Methods: Lipoaspirate from eight subjects...