A5032939376- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Angiogenesis and VEGF in Cancer
- Hematopoietic Stem Cell Transplantation
- Erythrocyte Function and Pathophysiology
- Congenital heart defects research
- Pluripotent Stem Cells Research
- T-cell and B-cell Immunology
- Phagocytosis and Immune Regulation
- Pregnancy and preeclampsia studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Sarcoma Diagnosis and Treatment
- Developmental Biology and Gene Regulation
- Cancer Cells and Metastasis
- Renal and related cancers
- Aquaculture disease management and microbiota
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Knee injuries and reconstruction techniques
Centre National de la Recherche Scientifique
2011-2023
Laboratoire de Biologie du Développement
2012-2023
Sorbonne Université
2012-2023
Institut de Biologie Paris-Seine
2021-2023
Inserm
2016-2023
Royal Netherlands Academy of Arts and Sciences
2020-2022
University Medical Center Utrecht
2014-2022
Hubrecht Institute for Developmental Biology and Stem Cell Research
2014-2022
Nantes Université
2011
Abstract Haematopoietic stem cells (HSCs) are generated from haemogenic endothelial (HE) via the formation of intra-aortic haematopoietic clusters (IAHCs) in vertebrate embryos. The molecular events controlling specification, endothelial-to-haematopoietic transition (EHT) and IAHC formation, as it occurs vivo inside aorta, still poorly understood. To gain insight these processes, we performed single-cell RNA-sequencing non-HE cells, HE undergoing EHT, whole IAHCs isolated mouse embryo...
Microglia, the tissue-resident macrophages of CNS, represent major targets for therapeutic intervention in a wide variety neurological disorders. Efficient reprogramming protocols to generate microglia-like cells vitro using patient-derived induced pluripotent stem will, however, require precise understanding cellular and molecular events that instruct microglial cell fates. This remains challenge since developmental origin microglia during embryogenesis is controversial. Here, genetic...
Abstract Positional information driving limb muscle patterning is contained in connective tissue fibroblasts but not myogenic cells. Limb muscles originate from somites, while tissues lateral plate mesoderm. With cell and genetic lineage tracing we challenge this model identify an unexpected contribution of plate-derived to the lineage, preferentially at myotendinous junction. Analysis single-cell RNA-sequencing data whole limbs successive developmental stages identifies a population...
We have combined the use of mouse genetic strains and mouse-into-chicken chimera system to determine precisely sequence forelimb colonization by presomitic mesoderm (PSM)-derived myoblasts angioblasts, possible role this latter cell type in myoblast guidance. By creating a new Flk1/Pax3 double reporter line, we established precise timetable for angioblast delamination/migration from somite limb bud. This was conserved when PSM grafted into chicken host, which further validates experimental...
Hematopoietic stem cells (HSCs), responsible for blood cell production, are generated during embryonic development. Human and chicken embryos share features that pose the as a reliable accessible alternative model to study developmental hematopoiesis. However, existence of HSCs has never been formally proven in embryos. Here, we established complete cartography quantification hematopoietic aorta We demonstrated bona fide HSCs, originating from embryo (and not yolk sac, allantois or head),...
CD105 is an auxiliary receptor for the transforming growth factor beta superfamily, highly expressed on proliferating endothelial cells and adult hematopoietic stem cells. Because mRNA expression was reported in developing aortic region, we further characterized its profile aorta examined potential of CD105+ Aortic cells, intra-aortic cell clusters purified fraction enriched progenitor/hematopoietic activity CD105. short-term clonogenic progenitors were CD105intermediate population whereas...
Adult-type hematopoietic stem and progenitor cells are formed during ontogeny from a specialized subset of endothelium, termed the hemogenic via an endothelial-to-hematopoietic transition (EHT) that occurs in embryonic aorta associated arteries. Despite efforts to generate models, little is known about mechanisms drive endothelial fate subsequent molecular control EHT. Here, we have designed stromal line-free controlled culture system utilizing pre-somitic mesoderm obtain large numbers...
ABSTRACT Recent studies have highlighted the crucial role of aorta microenvironment in generation first haematopoietic stem cells (HSCs) from specialized haemogenic endothelial (HECs). Despite more than two decades investigations, we require a better understanding cellular and molecular events driving formation polarization, which will be pivotal to establish mechanisms that operate during HEC specification HSC competency. Here, outline early involved vertebrate by comparing four different...
Hematopoietic stem cells (HSCs) express a large variety of cell surface receptors that are associated with acquisition self-renewal and multipotent properties. Correct expression these depends on delicate balance between trafficking, recycling, degradation is controlled by the microtubule network Golgi apparatus, whose roles have hardly been explored during embryonic/fetal hematopoiesis. Here we show that, in absence CLASP2, microtubule-associated protein, overall production HSCs reduced,...
Abstract Positional information driving limb muscle patterning is contained in lateral plate mesoderm-derived tissues, such as tendon or connective tissue but not myogenic cells themselves. The long-standing consensus that originate from the somitic mesoderm, while fibroblasts mesoderm. We challenged this model using cell and genetic lineage tracing experiments birds mice, respectively, identified a subpopulation of at tips close to tendons originating mesoderm derived gene lineages....
Event Abstract Back to Embryonic microglia derive from primitive macrophages and are replaced by cmyb-dependent definitive in zebrafish Giuliano Ferrero1, 2*, Chris Mahony3, 4, Eleonore Dupuis1, 2, Laurent Yvernogeau5, Elodie Di Ruggiero1, Magali Miserocchi1, Marianne Caron1, Catherine Robin5, David Traver6, Julien Y. BERTRAND4 Valerie Wittamer1, 2* 1 Free University of Brussels, Belgium 2 Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire, 3 Université Genève,...
ABSTRACT The presomitic mesoderm (PSM) is initially an unsegmented structure localized on each side of the neural tube developing embryo, which progressively segments to form somites. somites will segregate and partition generate dorsal dermomyotome ventral sclerotome. Endothelial myogenic cells both trunk limbs are derived from There a lack efficient reporter mouse models label trace PSM derivatives, despite their crucial contribution many developmental processes. In this study, we...