A5074144867- Developmental Biology and Gene Regulation
- Hedgehog Signaling Pathway Studies
- Glioma Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Congenital heart defects research
- TGF-β signaling in diseases
- Renal and related cancers
- Retinoids in leukemia and cellular processes
- Epigenetics and DNA Methylation
- Planarian Biology and Electrostimulation
- Neurogenesis and neuroplasticity mechanisms
- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Sarcoma Diagnosis and Treatment
- Marine Ecology and Invasive Species
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Autism Spectrum Disorder Research
- RNA modifications and cancer
- Wind Turbine Control Systems
- Hippo pathway signaling and YAP/TAZ
- Genetics and Neurodevelopmental Disorders
- Axon Guidance and Neuronal Signaling
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
Centre National de la Recherche Scientifique
2006-2024
Université Paris Cité
2015-2024
Institut Jacques Monod
2017-2024
Inserm
2005-2023
Centre de Recherche en Cancérologie de Lyon
2023
Université Claude Bernard Lyon 1
2023
Convergence
2023
Hospital Sant Joan de Déu Barcelona
2023
Hôpital Femme Mère Enfant
2023
Centre Hospitalier Universitaire de Lille
2023
SummarySecreted signals, known as morphogens, provide the positional information that organizes gene expression and cellular differentiation in many developing tissues. In vertebrate neural tube, Sonic Hedgehog (Shh) acts a morphogen to control pattern of neuronal subtype specification. Using an vivo reporter Shh signaling, mouse genetics, systems modeling, we show spatially temporally changing gradient signaling is interpreted by regulatory logic downstream transcriptional network. The...
Morphogens are secreted signalling molecules that act in a graded manner to control the pattern of cellular differentiation developing tissues. An example is Sonic hedgehog (Shh), which acts several vertebrate tissues, including central nervous system, provide positional information during embryonic patterning. Here we address how Shh assigns identities distinct neuronal subtype progenitors throughout ventral neural tube. Assays intracellular signal transduction and gene expression indicate...
The secreted ligand Sonic Hedgehog (Shh) organizes the pattern of cellular differentiation in ventral neural tube. For five neuronal subtypes, increasing levels and durations Shh signaling direct progenitors to progressively more identities. Here we demonstrate that this mode action is not applicable generation most cell type, nonneuronal floor plate (FP). In chick mouse embryos, FP specification involves a biphasic response controls dynamic expression key transcription factors. During...
Bone morphogenetic proteins (BMPs) are secreted regulators of cell fate in several developing tissues. In the embryonic spinal cord, they control emergence neural crest, roof plate and distinct subsets dorsal interneurons. Although a gradient BMP activity has been proposed to determine type identity vivo, whether this is sufficient for pattern formation vitro unclear. Here, we demonstrate that exposure BMP4 initiates spatial dynamics signalling within self-emerging epithelia both mouse human...
Abstract We characterized 79 microsatellite DNA markers, which were obtained from genomic libraries enriched for CA, GA, ATG and TAGA motif repeats, in the Pacific oyster Crassostrea gigas . For eight F 1 grandparents or great‐grandparents of mapping families, average heterozygosity, 0.705, number alleles per locus, 5.7, did not vary among motif‐repeat motif‐complexity categories. Non‐amplifying polymerase chain reaction null alleles, confirmed by segregation detected at 41 (51.9%) loci....
Although retinoic acid (RA) has been implicated as one of the diffusible signals regulating forebrain development, patterning not analyzed in detail knockout mouse mutants deficient embryonic RA synthesis. We show that retinaldehyde dehydrogenase 2 (RALDH2) enzyme is responsible for synthesis craniofacial region and between 8- 15-somite stages. Raldh2-/- embryos exhibit defective morphogenesis various derivatives,including ventral diencephalon, optic telencephalic vesicles. These defects are...
The progressive generation of embryonic trunk structures relies on the proper patterning caudal epiblast, which involves integration several signalling pathways. We have investigated function retinoic acid (RA) during this process. show that, in addition to posterior mesendoderm, primitive streak and node cells transiently express RA-synthesizing enzyme Raldh2 prior headfold stage. RA-responsive (detected by RA-activated RARE-lacZtransgene) are additionally found epiblast layer. Analysis...
Sonic hedgehog signalling is essential for the embryonic development of many tissues including central nervous system, where it controls pattern cellular differentiation. A genome-wide screen neural progenitor cells to evaluate Shh signalling-regulated transcriptome identified forkhead transcription factor Foxj1. In both chick and mouse Foxj1 expressed in ventral midline tube that make up floor plate. Consistent with role formation long motile cilia, plate produce cilia are longer than...
Rostro-caudal patterning of vertebrates depends on the temporally progressive activation HOX genes within axial stem cells that fuel embryo elongation. Whether pace sequential genes, 'HOX clock', is controlled by intrinsic chromatin-based timing mechanisms or temporal changes in extrinsic cues remains unclear. Here, we studied clock pacing human pluripotent cell-derived progenitors differentiating into diverse spinal cord motor neuron subtypes. We show caudal a dynamic increase FGF...
ABSTRACT The capacity to regenerate lost tissues varies significantly among animals. Some phyla, such as the annelids, display substantial regenerating abilities, although little is known about cellular mechanisms underlying process. To precisely determine origin, plasticity and fate of cells participating in blastema formation posterior end regeneration after amputation annelid Platynereis dumerilii, we developed specific tools track different cell populations. Using these tools, find that...
Abstract Understanding how transcription factors regulate organized cellular diversity in developing tissues remains a major challenge due to their pleiotropic functions. We addressed this by monitoring and genetically modulating the activity of PAX3 PAX7 during specification neural progenitor pools embryonic spinal cord. Using mouse models, we show that balance between transcriptional activating repressing functions these is modulated along dorsoventral axis instructive patterning pools. By...
Retinoic acid (RA) has been implicated as one of the signals providing a posterior character to developing vertebrate central nervous system. Embryonic RA first appears in region gastrulating embryo up node level, where it may signal within adjacent epiblast and/or newly induced neural plate induce hindbrain and spinal cord fate. Conversely, rostral head development requires forebrain-inducing produced by anterior visceral endoderm prechordal mesoderm, there is evidence that receptors must...
Pattern formation in developing tissues is driven by the interaction of extrinsic signals with intrinsic transcriptional networks that together establish spatially and temporally restricted profiles gene expression. How this process orchestrated at molecular level genomic cis-regulatory modules one central questions developmental biology. Here we have addressed analysing regulation Pax3 expression context spinal cord. induced early during neural development progenitors dorsal cord maintained...
Summary Fetal valproate syndrome (FVS) is caused by in utero exposure to the drug sodium valproate. Valproate used worldwide for treatment of epilepsy, as a mood stabiliser and its pain relieving properties. In addition birth defects, FVS associated with an increased risk autism spectrum disorder (ASD), which characterised abnormal behaviours. perturbs multiple biochemical pathways alters gene expression through inhibition histone deacetylases. Which, if any, these mechanisms relevant...
The establishment of separated pulmonary and systemic circulation in vertebrates, via cardiac outflow tract (OFT) septation, is a sensitive developmental process accounting for 10% all congenital anomalies. Neural Crest Cells (NCC) colonising the heart condensate along primitive endocardial tube force its scission into two tubes. Here, we show that NCC aggregation progressively decreases OFT distal-proximal axis following BMP signalling gradient. Dullard, nuclear phosphatase, tunes gradient...
Dorsal spinal neurogenesis is orchestrated by the combined action of signals secreted from roof plate organizer and a downstream transcriptional cascade. Within this cascade, Msx1 Msx2, two homeodomain transcription factors (TFs), are induced earlier than bHLH neuralizing TFs. Whereas TFs have been shown to specify neuronal cell fate, function Msx genes remains poorly defined. We describe dramatic alterations patterning in Msx1/Msx2 double-mutant mouse embryos. The most dorsal progenitor...
Specification of neurons in the spinal cord relies on extrinsic and intrinsic signals, which turn are interpreted by expression transcription factors. V2 interneurons develop from ventral aspects cord. We report here a novel neuronal subtype, named V2s, zebrafish embryos. Formation these depends factors sox1a sox1b. They common gata2a- gata3-dependent precursors co-expressing markers V2b V2s interneurons. Chemical blockage Notch signalling causes decrease an increase cells. Our results...
The development of a functional tissue requires coordination the amplification progenitors and their differentiation into specific cell types. molecular basis for this during myotome ontogeny is not well understood. Dermomytome that colonize first acquire myocyte identity subsequently proliferate as Pax7-expressing before undergoing terminal differentiation. We show dynamics sonic hedgehog (Shh) signaling crucial transition in both avian mouse embryos. Initially, Shh ligand emanating from...