A5079210684- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- RNA Research and Splicing
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Developmental Biology and Gene Regulation
- Hedgehog Signaling Pathway Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA and protein synthesis mechanisms
- Planarian Biology and Electrostimulation
- Mitochondrial Function and Pathology
- Melanoma and MAPK Pathways
- Microtubule and mitosis dynamics
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- Prion Diseases and Protein Misfolding
- Cervical Cancer and HPV Research
- Cancer Mechanisms and Therapy
- Phytochemistry and Biological Activities
- Congenital Ear and Nasal Anomalies
- Protein Degradation and Inhibitors
- Genetics, Aging, and Longevity in Model Organisms
Université Paris Cité
2012-2024
Inserm
2012-2024
Université Paris-Saclay
2021-2024
CEA Paris-Saclay
2023-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2013-2024
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2012-2024
Austrian Cluster for Tissue Regeneration
2023-2024
Paracelsus Medical University
2023-2024
Institut de Radiobiologie Cellulaire et Moléculaire
2012-2023
Stanford University
2023
Research Article25 March 2013Open Access Source Data Vascular-derived TGF-β increases in the stem cell niche and perturbs neurogenesis during aging following irradiation adult mouse brain Jose R. Pineda CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, Fontenay-aux-Roses, France INSERM, U967, Université Paris Diderot, Sorbonne Cité, UMR 967, Sud, Search for more papers by this author Mathieu Daynac Alexandra Chicheportiche Arantxa Cebrian-Silla Laboratorio Neurobiología Comparada, Instituto...
Abstract Although neural stem cells (NSCs) sustain continuous neurogenesis throughout the adult lifespan of mammals, they progressively exhibit proliferation defects that contribute to a sharp reduction in subventricular during aging. However, little is known regarding early age-related events neurogenic niches. Using fluorescence-activated cell sorting technique allows for prospective purification main populations from zone (SVZ), we demonstrated an decline with dramatic loss progenitor 4...
Highlights•Transcriptome analysis reveals molecular hallmarks of activated and quiescent NSCs•Data resource putative markers and/or regulators NSC quiescence•Quiescent NSCs integrate various signals from the microenvironment•Syndecan-1 is involved in proliferation NSCsSummaryDeciphering mechanisms that regulate quiescence adult neural stem cells (NSCs) crucial for development therapeutic strategies based on stimulation their endogenous regenerative potential damaged brain. We show LeXbright...
Rostro-caudal patterning of vertebrates depends on the temporally progressive activation HOX genes within axial stem cells that fuel embryo elongation. Whether pace sequential genes, 'HOX clock', is controlled by intrinsic chromatin-based timing mechanisms or temporal changes in extrinsic cues remains unclear. Here, we studied clock pacing human pluripotent cell-derived progenitors differentiating into diverse spinal cord motor neuron subtypes. We show caudal a dynamic increase FGF...
The prion protein (PrP) is highly conserved and ubiquitously expressed, suggesting that it plays an important physiological function.However, despite decades of investigation, this role remains elusive.Here, by using animal cellular models, we unveil a key PrP in the DNA damage response.Exposure neurons to genotoxic stress activates PRNP transcription leading increased amount nucleus where interacts with APE1, major mammalian endonuclease essential for base excision repair, stimulates its...
Neurogenesis decreases during aging causing a progressive cognitive decline but it is still controversial whether proliferation defects in neurogenic niches result from loss of neural stem cells or an impairment their progression through the cell cycle. Using accurate fluorescence-activated sorting technique, we show that pool maintained subventricular zone middle-aged mice while they have reduced proliferative potential eventually leading to subsequent decrease progeny. In addition,...
Cervical carcinomas result from cellular transformation by the human papillomavirus (HPV) E6 and E7 oncogenes which are constitutively expressed in cancer cells. The oncogene degrades p53 thereby modulating a large set of target genes as shown previously cervical carcinoma cell line HeLa. Here we show that TAp63β isoform p63 transcription factor is also E6. gene plays an essential role skin homeostasis at least six isoforms. One these isoforms, ΔNp63α, has been found overexpressed squamous...
Inhibitors of BRAFV600E kinase are currently under investigations in preclinical and clinical studies involving glioma. Studies demonstrated response to such individualized therapy the majority patients whereas some tumors continue grow despite treatment. To study resistance mechanisms, which include feedback activation mitogen-activated protein (MAPK) signaling melanoma, we developed a luciferase-modified cell line (2341luc) from BrafV600E mutant Cdkn2a- deficient murine high-grade glioma,...
Essential oils (EOs) are vastly used as natural antibiotics in Complementary and Alternative Medicine (CAM). Their intrinsic chemical variability synergisms/antagonisms between its components make difficult to ensure consistent effects through different batches. Our aim is evaluate the use of artificial neural networks (ANNs) for prediction their antimicrobial activity. Methods. The composition activity 49 EOs, extracts, and/or fractions was extracted from NCCLS compliant works. fast (FANN)...
Neural stem cells (NSCs) in the subventricular zone of lateral ventricles (SVZ) sustain olfactory neurogenesis throughout life mammalian brain. They successively generate transit amplifying (TACs) and neuroblasts that differentiate into neurons once they integrate bulbs. Emerging fluorescent activated cell sorting (FACS) techniques have allowed isolation NSCs as well their progeny started to shed light on gene regulatory networks adult neurogenic niches. We report here a technique allows...
Abstract Oligodendrocyte progenitor cells (OPC) undergo asymmetric cell division (ACD) to generate one OPC and differentiating oligodendrocyte (OL) progeny. Loss of pro-mitotic proteoglycan marker NG2 in the OL progeny is earliest immunophenotypic change unknown mechanism that indicates differentiation commitment. Here, we report expression mouse homolog Drosophila tumor suppressor Lethal giant larvae 1 (Lgl1) induced during differentiation. Lgl1 conditional knockout retain show reduced...
Astrocytes are glial cells proposed as the main Sonic hedgehog (Shh)-responsive in adult brain. Their roles mediating Shh functions still poorly understood. In hypothalamus, astrocytes support neuronal circuits implicated regulation of energy metabolism. this study, we investigated impact genetic activation signaling on hypothalamic and characterized its effects We analyzed distribution gene transcripts pathway (Ptc, Gli1, Gli2, Gli3) using single molecule fluorescence situ hybridization...
ABSTRACT BRAF V600E pediatric low-grade gliomas frequently transform into high-grade (HGG) and poorly respond to chemotherapy, resulting in high mortality. Although combined MEK inhibition (BRAFi+MEKi) outperforms ∼70% of HGG patients are therapy resistant undergo unbridled tumor progression. glioma have an immune-rich microenvironment suggesting that they could be responsive immunotherapy but effects BRAFi+MEKi on anti-tumor immunity unclear. Using patient tissue before after BRAFi+MEKi,...
SUMMARY Rostro-caudal patterning of vertebrates depends on the temporally progressive activation HOX genes within axial stem cells that fuel embryo elongation. Whether sequential activation, “ clock”, is paced by intrinsic chromatin-based timing mechanisms or temporal changes in extrinsic cues remains unclear. Here, we studied clock pacing human pluripotent differentiating into spinal cord motor neuron subtypes which are progenies progenitors. We show caudal progenitors controlled a dynamic...
The lateral wall of the subventricular zone harbors neural stem cells (NSC, B cells) which generate proliferating transient-amplifying progenitors (TAP, C that ultimately give rise to neuroblasts (NB, A cells). Molecular profiling at single cell level struggles distinguish these different types. Here, we combined transcriptome analyses FACS-sorted and single-cell RNAseq demonstrate existence an abundant, clonogenic multipotent population immature (iNB transition between TAP migrating NB...
The lateral wall of the mouse subventricular zone harbors neural stem cells (NSC, B cells) which generate proliferating transient-amplifying progenitors (TAP, C that ultimately give rise to neuroblasts (NB, A cells). Molecular profiling at single-cell level struggles distinguish these different cell types. Here, we combined transcriptome analyses FACS-sorted and RNAseq demonstrate existence an abundant, clonogenic multipotent population immature (iNB transition between TAP migrating NB...
Neural stem cells (NSCs) in the subventricular zone of lateral ventricles (SVZ) sustain olfactory neurogenesis throughout life mammalian brain. They successively generate transit amplifying (TACs) and neuroblasts that differentiate into neurons once they integrate bulbs. Emerging fluorescent activated cell sorting (FACS) techniques have allowed isolation NSCs as well their progeny started to shed light on gene regulatory networks adult neurogenic niches. We report here a technique allows...