A5015274570- Glioma Diagnosis and Treatment
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Immune cells in cancer
- RNA Research and Splicing
- Periodontal Regeneration and Treatments
- CAR-T cell therapy research
- Phagocytosis and Immune Regulation
- Preterm Birth and Chorioamnionitis
- RNA and protein synthesis mechanisms
- Reproductive System and Pregnancy
- Sepsis Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Gut microbiota and health
- Fungal and yeast genetics research
- Eicosanoids and Hypertension Pharmacology
- Oral and Maxillofacial Pathology
- DNA Repair Mechanisms
- Melanoma and MAPK Pathways
- Mesenchymal stem cell research
- Protease and Inhibitor Mechanisms
- Dental Trauma and Treatments
- Heat shock proteins research
- Hippo pathway signaling and YAP/TAZ
- interferon and immune responses
Stanford University
2014-2023
Palo Alto University
2022-2023
IGM Biosciences (United States)
2020
Cardiovascular Institute of the South
2014
University of California, San Francisco
1996-2011
University of California, Los Angeles
2000-2005
Genetic variation at the chromosome 9p21 risk locus promotes cardiovascular disease; however, it is unclear how or which proteins encoded this contribute to disease. We have previously demonstrated that loss of one candidate gene locus, cyclin-dependent kinase inhibitor 2B (Cdkn2b), in mice vascular SMC apoptosis and aneurysm progression. Here, we investigated role Cdnk2b atherogenesis found a mouse model atherosclerosis, deletion promoted advanced development atherosclerotic plaques...
Abstract Single‐cell technologies have immense potential to shed light on molecular and biological processes that drive human diseases. Mass cytometry (or Cytometry by Time Of Flight mass spectrometry, CyTOF) has already been employed in clinical studies comprehensively survey patients’ circulating immune system. As interest the “bedside” application of is growing, delineation relevant methodological issues called for. This report uses a newly generated dataset discuss important...
F ibronectin (FN) is an extracellular matrix (ECM) molecule important in cell adhesion and migration wound healing. It also likely periodontal ligament (PDL) cell–ECM interactions, thus regenerating tissues. In this study we characterized PDL cells their interactions with FN, testing different isolates taken from healthy diseased conditions. were by morphology, integrin profile, motility, bone nodule formation. Cells then assayed for adhesion, proliferation, Chemotaxis response to FN or...
Abstract Preterm labor and infections are the leading causes of neonatal deaths worldwide. During pregnancy, immunological cross talk between mother her fetus is critical for maintenance pregnancy delivery an immunocompetent neonate. A precise understanding healthy fetomaternal immunity important first step to identifying dysregulated immune mechanisms driving adverse maternal or outcomes. This study combined single-cell mass cytometry paired peripheral umbilical cord blood samples from...
Myxococcus xanthus is a gliding bacterium that possesses two motility systems, the adventurous (A-motility) and social (S-motility) systems. A-motility used for individual cell gliding, while S-motility in multicellular groups. Video microscopy studies showed nla24 cells are non-motile on agar surfaces, suggesting gene product absolutely required both under these assay conditions. requires functional type IV pili, wild-type LPS O-antigen, an extracellular matrix of exopolysaccharide (EPS)...
Volatile sulfur compounds such as hydrogen sulfide and methyl mercaptan have been associated with adult periodontitis well healing surgical wounds. To examine the effects of these on periodontium, we assayed periodontal ligament (PDL) cells for changes in intracellular pH, total protein, cell migration following chronic exposure to CH3SH. Intracellular pH was quantitated by fluorescence measurements loaded BCECF, a pH-sensitive dye. Data show that 48-hour lowered resting but did not...
AbstractCDC5 proteins are components of the pre-mRNA splicing complex and essential for cell cycle progression in yeast, plants mammals. Human CDC5 is phosphorylated a mitogen-dependent manner, its association with spliceosome ATP-dependent. Examination amino acid sequence suggests that CDC5L may be at up to 28 potential consensus recognition sequences known kinases, however, identity actual phosphorylation sites, their role regulating activity, kinases responsible have not previously been...
Abstract Application of high-content immune profiling technologies has enormous potential to advance medicine. Whether these reveal pertinent biology when implemented in interventional clinical trials is an important question. The beneficial effects preoperative arginine-enriched dietary supplements (AES) are highly context specific, as they reduce infection rates elective surgery, but possibly increase morbidity critically ill patients. This study combined single-cell mass cytometry with...
ABSTRACT Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient social motility fruiting body development. We compared the two loci by genetic mapping, complementation with a cosmid clone, DNA sequencing, gene disruption found 16 of 18 alleles map to genes. Another allele contains mutation sglK gene. About 36.6 kb around dsp-dif locus was sequenced annotated, 50% genes novel.
Survivin (SVV) is a multifunctional protein that has been implicated in the development of neointimal hyperplasia. Nuclear SVV essential for mitosis, whereas mitochondria cytoprotective function. Here, we investigated effects RNA interference (RNAi)-mediated knockdown on cell cycle kinetics, apoptosis, migration, and gene expression primary cultured vascular smooth muscle cells (VSMCs) from human saphenous vein. Primary Human VSMCs were obtained veins under standard conditions. was achieved...
Myxococcus xanthus is a Gram-negative gliding bacterium that aggregates and develops into multicellular fruiting bodies in response to starvation. Two chemosensory systems (frz dif), both of which are homologous known chemotaxis proteins, were previously identified through characterization various developmental mutants. This study aims examine the interaction between these two since them required for body formation M. xanthus. Through detailed phenotypic analyses frz dif double mutants, we...
The dif operon is essential for fruiting body formation, fibril (exopolysaccharide) production and social motility of Myxococcus xanthus . locus contains a gene cluster homologous to chemotaxis genes such as mcp ( difA ), cheW difC cheY difD cheA difE ) cheC difF well an unknown ORF called difB This study used yeast two-hybrid analysis investigate possible interactions between Dif proteins, determined that DifA, C, D E interact in similar fashion proteins Escherichia coli Bacillus subtilis...
ABSTRACT BRAF V600E pediatric low-grade gliomas frequently transform into high-grade (HGG) and poorly respond to chemotherapy, resulting in high mortality. Although combined MEK inhibition (BRAFi+MEKi) outperforms ∼70% of HGG patients are therapy resistant undergo unbridled tumor progression. glioma have an immune-rich microenvironment suggesting that they could be responsive immunotherapy but effects BRAFi+MEKi on anti-tumor immunity unclear. Using patient tissue before after BRAFi+MEKi,...
The study of complex cardiovascular disease (CVD) has been hampered by the lack appropriate human cellular model systems. In response, NHBLI sponsored NextGen Consortium, which encompasses 9 independent efforts spanning portfolio NHLBI related phenotypes. goals consortium include: 1. To develop and improve methods for large-scale production characterization induced pluripotent stem cell (iPSC) models CVD; 2. create a resource iPSC lines from large number phenotypically genotypically...
Abstract Traumatic injury produces a profound immune response that mobilizes both innate and adaptive branches of the system. Patient recovery after major trauma is highly variable. Protracted surgery -a trauma- causes significant societal economic costs. However, mechanistic underpinning remains poorly understood. We recently applied high-parameter mass cytometry at bedside for deep profiling patients undergoing orthopedic surgery1. The data revealed patient-specific responses in monocyte...
Abstract Novel treatment approaches are urgently needed for pediatric central nervous system (CNS) tumors as they the leading cause of cancer-related deaths in children. A lack research materials impedes progress towards developing such therapies. Although various brain cancer biobanks exist, these rarely store viable patient-derived tissue and cells live cell analyses, including fate assays testing drug sensitivities. We propose that a biorepository dissociates broadly representing CNS...
Abstract Hemispherian is advancing first-in-class therapeutics for cancers that are historically difficult to treat. Glioblastoma multiforme (GBM) the primary indication our lead compound GLIX1, which currently in late-stage preclinical development. GLIX1 developed together with a biomarker-based companion diagnostic assay pair diagnostics and optimal treatment. It Hemispherian’s mission provide superior treatment options brain cancer patients. proprietary molecules selectively target DNA...
Abstract In the central nervous system (CNS), gliomas represent almost 50% of all pediatric tumors and due to their complex histological biological heterogeneity, aggressive standard treatment consisting surgery chemoradiotherapy remains ineffective. As a result, more biologically based therapies are urgently needed this requires deeper knowledge etiology biology gliomas, which can arise from oligodendrocyte progenitor cells (OPCs). OPCs most proliferative cell type in CNS, upon oncogenic...
<h3>Background</h3> Therapeutic antibodies inhibiting PD-1/PD-L1 have demonstrated clinical efficacy though only a fraction of patients respond. Combinations are being explored to enhance responses including anti-PD-1/PD-L1 IgG with IL-15-pathway stimulating agents remove PD-1 immunosuppressive signaling and anti-tumor NK memory CD8 T cell expansion survival. We engineered an anti-PD-L1 pentameric high affinity, avidity IgM, target low PD-L1 expressing tumors, IL-15 superagonist fused the...