A5078874139- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Alzheimer's disease research and treatments
- Trace Elements in Health
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Dementia and Cognitive Impairment Research
- Dermatology and Skin Diseases
- Amino Acid Enzymes and Metabolism
- RNA regulation and disease
- Asthma and respiratory diseases
- T-cell and B-cell Immunology
- Advanced Neuroimaging Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Animal Disease Management and Epidemiology
- Parasites and Host Interactions
- Infectious Encephalopathies and Encephalitis
- Immunotherapy and Immune Responses
- Coccidia and coccidiosis research
- Nanopore and Nanochannel Transport Studies
- Escherichia coli research studies
- Antimicrobial Peptides and Activities
- Leprosy Research and Treatment
- Microfluidic and Bio-sensing Technologies
- Genomics, phytochemicals, and oxidative stress
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2024
CEA Paris-Saclay
2019-2024
Université Paris-Saclay
2019-2022
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2008-2021
Direction de la Recherche Fondamentale
2017-2021
Infectious Disease Models and Innovative Therapies
2008-2015
Seminario Permanente de Investigación Agraria
2014-2015
CEA Grenoble
2009-2012
CEA Cadarache
2009-2012
Ruhr University Bochum
2010
Abstract The inactivation of bacteria and biomolecules using plasma discharges were investigated within the European project BIODECON. goal was to identify isolate mechanisms by combining dedicated beam experiments with especially designed reactors. reactors are based on a fully computer‐controlled, low‐pressure inductively‐coupled (ICP). Four these built distributed among consortium, thereby ensuring comparability results between teams. Based this combined effort, role UV light, chemical...
The emergence of variant Creutzfeldt Jakob Disease (vCJD) is considered a likely consequence human dietary exposure to Bovine Spongiform Encephalopathy (BSE) agent. More recently, secondary vCJD cases were identified in patients transfused with blood products prepared from apparently healthy donors who later went on develop the disease. As there no validated assay for detection vCJD/BSE infected individuals prevalence disease population remains uncertain. In that context, risk borne...
Background Human variant Creutzfeldt-Jakob Disease (vCJD) results from foodborne transmission of prions slaughtered cattle with classical Bovine Spongiform Encephalopathy (cBSE). Atypical forms BSE, which remain mostly asymptomatic in aging cattle, were recently identified at slaughterhouses throughout Europe and North America, raising a question about human susceptibility to these new prion strains. Methodology/Principal Findings Brain homogenates BSE atypical (BASE) infections inoculated...
Classical bovine spongiform encephalopathy (c-BSE) is the only animal prion disease reputed to be zoonotic, causing variant Creutzfeldt-Jakob (vCJD) in humans and having guided protective measures for human health against diseases. Recently, partial transmissions humanized mice showed that zoonotic potential of scrapie might similar c-BSE. We here report direct transmission a natural classical isolate cynomolgus macaque, highly relevant model diseases, after 10-year silent incubation period,...
The prion protein (PrP) is highly conserved and ubiquitously expressed, suggesting that it plays an important physiological function.However, despite decades of investigation, this role remains elusive.Here, by using animal cellular models, we unveil a key PrP in the DNA damage response.Exposure neurons to genotoxic stress activates PRNP transcription leading increased amount nucleus where interacts with APE1, major mammalian endonuclease essential for base excision repair, stimulates its...
Alzheimer's disease is characterized by cognitive alterations, cerebral atrophy and neuropathological lesions including neuronal loss, accumulation of misfolded aggregated β-amyloid peptides (Aβ) tau proteins. Iatrogenic induction Aβ suspected in patients exposed to pituitary-derived hormones, dural grafts, or surgical instruments, presumably contaminated with Aβ. Induction has been demonstrated transgenic mice after contamination brain homogenates, very limited functional consequences....
The efficacy of a rapid test for detecting PrP(Sc) in central nervous system tissue was evaluated the postmortem diagnosis BSE at different times during course disease. One hundred and six samples brain, level medulla oblongata, spinal cord, derived from experimental study pathogenesis carried out Great Britain between 1991 1995, were examined. detected most exposed animals killed 32 months or more after they had been to agent, before onset clinical signs which first recorded 35 months....
The European Commission asked EFSA for a Scientific Opinion: to revise the state of knowledge about differences between chronic wasting disease (CWD) strains found in North America (NA) and Europe within Europe; review new scientific evidence on zoonotic potential CWD provide recommendations address risks identify risk factors spread Union. Full characterisation isolates is being pursued, whereas most NA have not been characterised this way. differing surveillance programmes these continents...
Abstract A common property of allergens is their potential to generate type 2 cytokine responses. To understand the mechanisms involved in this phenomenon, we have evaluated polarizing a major allergen, Dermatophagoides pteronyssinus 1 (Der p 1), an heterologous immunization system using glutathione S-transferase parasite Schistosoma mansoni (Sm28-GST) as immunogen. In previous studies, showed that with Sm28-GST emulsified CFA induced nonpolarized immune response. contrast, when alum was...
Polarization of the immune response towards Th1 or Th2 profiles is under control several, not yet well known, mechanisms. The present study was undertaken to investigate whether responses generated against major protein antigens, parasitic (Schistosoma mansoni) and bacterial (Clostridium tetani) origin, characteristic Th profiles. Mice were immunized with a single dose S. mansoni 28 kDa glutathione-S-transferase (Sm28-GST) tetanus toxin fragment c (TTc) in combination different adjuvants,...
Since the appearance of bovine spongiform encephalopathy (BSE) in cattle and its linkage with human variant Creutzfeldt-Jakob disease, possible spread this agent to sheep flocks has been concern as a potential new source contamination. Molecular analysis protease cleavage abnormal prion protein (PrP), by Western blotting (PrP(res)) or immunohistochemical methods (PrP(d)), shown some distinguish BSE scrapie sheep. Using newly developed enzyme-linked immunosorbent assay, we identified 18...
In most forms of prion diseases, blood is infectious, but detection by immunochemistry techniques the only available marker infection (the misfolded protein, PrPTSE) in remains elusive. We developed a novel method for PrPTSE prion-infected rodents based on finding that associated with plasma exosomes. However, further purification exosomes sucrose gradient was necessary to remove immunoglobulins, which interfere PrPTSE, masking its immunochemistry. Finally, we report about 20% infectivity
Gadolinium (Gd)-stained MRI is based on Gd contrast agent (CA) administration into the brain parenchyma. The strong signal increase induced by CA can be converted resolution enhancement to record microscopic MR images. Moreover, inhomogeneous distribution of in improves between different tissues and provides new contrasts Gd-stained detects amyloid plaques, one lesions Alzheimer's disease (AD), APPSL/PS1M146L mice or primates. Numerous transgenic with various plaque typologies have been...
Abstract Exposure of human populations to bovine spongiform encephalopathy through contaminated food has resulted in <250 cases variant Creutzfeldt–Jakob disease (vCJD). However, more than 99% vCJD infections could have remained silent suggesting a long-term risk secondary transmission particularly blood. Here, we present experimental evidence that transfusion mice and non-human primates blood products from symptomatic non-symptomatic infected donors induces not only vCJD, but also...
The aim of this work was to establish an immunological test suitable for specifically detecting PrPres in tissues from animals or humans developing TSEs. We chose use as detection method a conventional two-site immunometric assay (sandwich immunoassay) because over the last 20 years technique has clearly been shown be more sensitive and specific than other tests. have established numerous assays based on monoclonal antibodies measurement PrPsen various mammalian species (human, bovine,...
Background Variant C reutzfeldt‐ J akob disease ( vCJD ) is a fatal neurodegenerative infection that can be transmitted by blood and products from donors in the latent phase of disease. Currently, there no validated antemortem screening test. Several tests are under development. Any useful test must with disease‐relevant reference panels. Study Design Methods To generate materials, we infected four cynomolgus macaques macaque‐adapted brain homogenates. Blood was collected throughout...