ABSTRACT Free-living amoebae (FLA) are ubiquitous organisms that have been isolated from various domestic water systems, such as cooling towers and hospital networks. In addition to their own pathogenicity, FLA can also act Trojan horses be naturally infected with amoeba-resisting bacteria (ARB) may involved in human infections, pneumonia. We investigated the biodiversity of amoebal hosts a network. Using enrichment on nonnutrient agar, we 15 protist strains 200 (7.5%) samples. One...

The complex ecology of free-living amoebae (FLA) and their role in spreading pathogenic microorganisms through water systems have recently raised considerable interest. In this study, we investigated the presence FLA amoebae-resisting bacteria (ARB) at various stages a drinking plant fed with river water. We isolated amoebal species from several points within plant, mostly early steps treatment. Echinamoeba- Hartmannella-related were mainly recovered whereas Acanthamoeba- Naegleria-related...

Monitoring of microbial changes during and after application various disinfection treatments in a model domestic water system.A pilot-scale system consisting seven galvanized steel re-circulation loops copper dead legs was constructed. Culture techniques, confocal laser scanning microscopy fluorescent situ hybridization viability staining with the BacLight LIVE/DEAD kit were used for planktonic biofilm flora monitoring. Before starting treatments, highly contaminated Legionella pneumophila...

Free-living amoebae that belong to the genus Acanthamoeba are widespread in environment, including water. They responsible for human infections and can host pathogenic microorganisms. Under unfavorable conditions, they form cysts with high levels of resistance disinfection methods, thus potentially representing a threat public health. In present study we evaluated efficacies various biocides against trophozoites several strains. We demonstrated disinfectant efficacy varied depending on...

Summary Large viruses infecting algae or amoebae belong to the NucleoCytoplasmic DNA Viruses (NCLDV) and present genotypic phenotypic characteristics that have raised major interest among microbiologists. Here, we describe a new large virus discovered in Acanthamoeba castellanii co‐culture of an environmental sample. The virus, referred as Lausannevirus, has very limited host range, spp. but being unable infect other mammalian cell lines tested. Within A. , this icosahedral about 200 nm...

The biocidal activity of peracetic acid (PAA) and benzalkonium chloride (BAC) on Pseudomonas aeruginosa biofilms was investigated by using a recently developed confocal laser scanning microscopy (CLSM) method that enables the direct real-time visualization cell inactivation within structure. This technique is based monitoring loss fluorescence corresponds to leakage fluorophore out cells due membrane permeabilization biocides. Although this approach has previously been used with success...

Despite huge interest, there are still no universally accepted standards to conduct clinical studies in the field of gut microbiota analysis. Stool material is frequently used as a proxy microbiota, but many different protocols can be for collection and DNA extraction. Whereas 16S rRNA encoding gene amplification sequencing has been widely study composition bacterial populations, it now being challenged by random, shotgun approach that brings far more information, although at higher cost. In...

Summary Accumulating evidence supports a role for Chlamydia ‐related organisms as emerging pathogens human and animals. Assessment of their pathogenicity requires strain availability, at least animal models serological studies. As these obligate intracellular species are able to grow inside amoebae, we used co‐culture with Acanthamoeba castellanii in an attempt recover new from river water. We isolated two strains eight water samples. The first is Parachlamydia acanthamoebae that differs...

Abstract We investigated the zoonotic potential of Waddlia chondrophila, a new Chlamydia-like abortigenic agent in ruminants. Anti-Waddlia antibody reactivity was tested by immunofluorescence and Western blot. seroprevalence higher women who had sporadic recurrent miscarriages than control (p<0.001). spp. may represent cause human fetal loss.

The development of a biofilm constitutes survival strategy by providing bacteria protective environment safe from stresses such as microbicide action and can thus lead to important health-care problems. In this study, resistance Bacillus subtilis strain (called hereafter NDmedical) recently isolated endoscope washer-disinfectors peracetic acid was investigated its ability protect the pathogen Staphylococcus aureus in mixed biofilms evaluated. Biocide within visualised real time using...

The incidence of lung and other diseases due to nontuberculous mycobacteria (NTM) is increasing. NTM sources include potable water, especially in households where populate pipes, taps, showerheads. share habitats with free-living amoebae (FLA) can grow FLA as parasites or endosymbionts. containing may form cysts that protect from disinfectants antibiotics. We first assessed the presence water biofilm samples collected a hospital, confirming high prevalence systems, particularly biofilms....

Leprosy is a curable neglected disease of humans caused by Mycobacterium leprae that affects the skin and peripheral nerves manifests clinically in various forms ranging from self-resolving, tuberculoid leprosy to lepromatous having significant pathology with ensuing disfiguration disability social stigma. Despite global success multi-drug therapy (MDT), incidences clinical have been observed individuals no apparent exposure other cases, suggestive possible non-human sources bacteria. In...

Abstract Background There is a growing interest in using gut commensal bacteria as “next generation” probiotics. However, this approach still hampered by the fact that there are few or no strains available for specific species difficult to cultivate. Our objective was adapt flow cytometry and cell sorting be able detect, separate, isolate, cultivate new of from fecal material. We focused on extremely oxygen sensitive (EOS) Faecalibacterium prausnitzii under-represented, health-associated...

The formation of multicellular communities known as biofilms is the part bacterial life cycle in which bacteria display cooperative behaviour and differentiated phenotypes leading to specific functions. Bacillus subtilis a Gram-positive bacterium that has served for decade model study molecular pathways control biofilm formation. Most data on B. have come from studies pellicles at air-liquid interface, or complex macrocolonies develop semi-solid nutritive agar. Here, using confocal laser...

The aim of this study was to compare the efficiency different disinfectants applicable Legionella control in domestic water systems. A supply simulation unit that allowed real-world conditions developed for purpose. system, consisting seven identical rigs, used treatment under equivalent system design, materials, hydraulics, quality, temperature and initial contamination. During study, each six loops received continuous application one following disinfectants: chlorine, electro-chlorination,...