A5019330137- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Neurogenesis and neuroplasticity mechanisms
- Hedgehog Signaling Pathway Studies
- Congenital heart defects research
- Epigenetics and DNA Methylation
- Spinal Cord Injury Research
- Angiogenesis and VEGF in Cancer
- Axon Guidance and Neuronal Signaling
- Cell Image Analysis Techniques
- Animal Genetics and Reproduction
- Circular RNAs in diseases
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Apelin-related biomedical research
- Single-cell and spatial transcriptomics
University of Lisbon
2017-2024
Faculdades Oswaldo Cruz
2023
Fundação Oswaldo Cruz
2023
Universidade de Brasília
2023
Instituto de Medicina Molecular João Lobo Antunes
2017-2022
Google (United States)
2018
Medical Research Council
2014
The Francis Crick Institute
2007
Instituto Gulbenkian de Ciência
2007
SummarySecreted signals, known as morphogens, provide the positional information that organizes gene expression and cellular differentiation in many developing tissues. In vertebrate neural tube, Sonic Hedgehog (Shh) acts a morphogen to control pattern of neuronal subtype specification. Using an vivo reporter Shh signaling, mouse genetics, systems modeling, we show spatially temporally changing gradient signaling is interpreted by regulatory logic downstream transcriptional network. The...
Kif7 may be the functional equivalent of Drosophila kinesin-like protein Costal2.
Development requires tissue growth as well cell diversification. To address how these processes are coordinated, we analyzed the development of molecularly distinct domains neural progenitors in mouse and chick tube. We show that during development, undergo changes size do not scale with overall size. Our data domain proportions first established by opposing morphogen gradients subsequently controlled domain-specific regulation differentiation rate but differences proliferation rate....
In the vertebrate neural tube, morphogen Sonic Hedgehog (Shh) establishes a characteristic pattern of gene expression. Here we quantify Shh gradient in developing mouse tube and show that while amplitude increases over time, activity pathway transcriptional effectors, Gli proteins, initially but later decreases. Computational analysis suggests three mechanisms could contribute to this adaptation: upregulation inhibitory receptor Ptch1, downregulation differential stability active inactive...
Homeodomain-containing transcription factors are important regulators of stem cell behavior. HOXB4 mediates expansion adult and embryo-derived hematopoietic cells (HSCs) when expressed ectopically. To define the underlying molecular mechanisms, we performed gene expression profiling in combination with subsequent functional analysis enriched HSCs embryonic derivatives expressing inducible HOXB4. Thereby, identified a set overlapping genes that likely represent "universal" targets A...
Zebrafish are able to regenerate the spinal cord and recover motor sensory functions upon severe injury, through activation of cells located at ependymal canal. Here, we show that surrounding canal in adult zebrafish express Foxj1a. We demonstrate Foxj1a from their birth embryonic neural tube activity is required for final positioning also response actively proliferate contribute restoration structure. Finally, this study reveals expression injured regulated by regulatory elements activated...
The vascular system is inefficiently repaired after spinal cord injury (SCI) in mammals, resulting secondary tissue damage and immune deregulation that contribute to the limited functional recovery. Unlike zebrafish can repair (SC) restore motility, but response has not been investigated. Here, we describe SC blood vasculature, starting development with initial vessel ingression a body size-dependent manner, acquisition of perivascular support establishment ventral dorsal circulation....
The vascular repair, a pivotal element of the spinal cord (SC) injury response, has been notably neglected in zebrafish research. Here, we detail extraction and dissection whole-mount SCs optimized immunofluorescence staining clearing protocols for visualization SC structures, such as network. Additional downstream applications these samples, from gelatin embedding to cryosectioning tissue staining, can be done after reversion are also described this work. For complete details on use...
"CLEM analysis of the angiogenic process in a zebrafish model spinal cord injury." Ultrastructural Pathology, 41(1), pp. 113–114
Abstract The vascular system is inefficiently repaired after spinal cord injury in mammals, resulting secondary tissue damage and immune deregulation that contribute to the limited functional recovery. Unlike zebrafish can repair restore motility, but response has not been investigated. Here we describe vasculature, from body size-dependent vessel ingression during development stereotypic organization barrier specialisation adulthood. After injury, vessels rapidly regrow into lesion,...