A5051171226- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Chemokine receptors and signaling
- Aquaculture disease management and microbiota
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Galectins and Cancer Biology
- Adipokines, Inflammation, and Metabolic Diseases
- Cell Adhesion Molecules Research
- HIV Research and Treatment
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- Glycosylation and Glycoproteins Research
- Signaling Pathways in Disease
- Neuroscience and Neuropharmacology Research
- Protease and Inhibitor Mechanisms
- Neuropeptides and Animal Physiology
- Lymphatic System and Diseases
- Silkworms and Sericulture Research
- Mast cells and histamine
- Histone Deacetylase Inhibitors Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
Université Libre de Bruxelles
2013-2025
University of Bristol
2024
Walloon Excellence in Lifesciences and Biotechnology
2018-2022
University of California, San Diego
2009-2013
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2001
CEA Paris-Saclay
2001
Interdisciplinary Scientific Research
2001
University of Pennsylvania
2000
Dendritic cells (DCs) and macrophages are professional antigen-presenting (APCs) that play key roles in both innate adaptive immunity. ChemR23 is an orphan G protein-coupled receptor related to chemokine receptors, which expressed specifically these cell types. Here we present the characterization of chemerin, a novel chemoattractant protein, acts through abundant diverse set human inflammatory fluids. Chemerin secreted as precursor low biological activity, upon proteolytic cleavage its...
The liver is the largest solid organ in body, yet it remains incompletely characterized. Here we present a spatial proteogenomic atlas of healthy and obese human murine combining single-cell CITE-seq, single-nuclei sequencing, transcriptomics, proteomics. By integrating these multi-omic datasets, provide validated strategies to reliably discriminate localize all hepatic cells, including population lipid-associated macrophages (LAMs) at bile ducts. We then align this across seven species,...
Chemerin is a chemotactic agent that was recently identified as the ligand of ChemR23, serpentine receptor expressed by activated macrophages and monocyte-derived dendritic cells (DCs). This paper shows blood plasmacytoid myeloid DCs express functional ChemR23. Recombinant chemerin induced transmigration across an endothelial cell monolayer. In secondary lymphoid organs (lymph nodes tonsils), ChemR23 CD123+ CD1a+ DC-SIGN+ in interfollicular T area. ChemR23+ were also observed dermis from...
Abstract Chemerin is the ligand of ChemR23 receptor and a chemoattractant factor for human immature dendritic cells (DCs), macrophages, NK cells. In this study, we characterized mouse chemerin/ChemR23 system in terms pharmacology, structure-function, distribution, vivo biological properties. Mouse chemerin synthesized as an inactive precursor (prochemerin) requiring, human, precise processing its C terminus generating agonist ChemR23. highly expressed plasmacytoid DCs at lower levels myeloid...
Abstract Dendritic cells and macrophages are professional APCs that play a central role in initiating immune responses, linking innate adaptive immunity. Chemerin is novel chemoattractant factor specifically attracts through its receptor ChemR23. Interestingly, chemerin secreted as precursor of low biological activity, prochemerin, which upon proteolytic removal C-terminal peptide, converted into potent highly specific agonist receptor. Given the fact often preceded by polymorphonuclear...
Chemerin is a novel protein identified as the natural ligand of ChemR23 (chemerinR), previously orphan G protein-coupled receptor expressed in immature dendritic cells and macrophages. synthesized secreted precursor, prochemerin, which poorly active, but converted into full agonist chemerinR by proteolytic removal last six amino acids. In present work, we have number peptides derived from C-terminal domain human prochemerin investigated their functional properties agonists or antagonists...
Microglia, the tissue-resident macrophages of CNS, represent major targets for therapeutic intervention in a wide variety neurological disorders. Efficient reprogramming protocols to generate microglia-like cells vitro using patient-derived induced pluripotent stem will, however, require precise understanding cellular and molecular events that instruct microglial cell fates. This remains challenge since developmental origin microglia during embryogenesis is controversial. Here, genetic...
The mononuclear phagocytic system consists of many cells, in particular macrophages, scattered throughout the body. However, there is increasing evidence for heterogeneity tissue-resident leading to a pressing need new tools discriminate subsets from other hematopoietic lineages. Macrophage-expressed gene (Mpeg)1.1 an evolutionary conserved encoding perforin-2, pore-forming protein associated with host defense against pathogens. Zebrafish mpeg1.1:GFP and mpeg1.1:mCherry reporters were...
CC-chemokine receptor 5 (CCR5) is the principal coreceptor for macrophage-tropic strains of human immunodeficiency virus type 1 (HIV-1). We have generated a set anti-CCR5 monoclonal antibodies and characterized them in terms epitope recognition, competition with chemokine binding, activation trafficking, activity. MC-4, MC-5, MC-7 mapped to amino-terminal domain, MC-1 second extracellular loop, MC-6 conformational covering multiple domains. inhibited regulated on normal T cell expressed...
CCR5 is a CC chemokine receptor expressed on memory lymphocytes, macrophages, and dendritic cells also constitutes the main coreceptor for macrophage-tropic (or R5) strains of human immunodeficiency viruses. In present study, we investigated whether was palmitoylated in its carboxyl-terminal domain by generating alanine substitution mutants three cysteine residues this region, individually or combination. We found that wild-type palmitoylated, but mutant lacking all Cys not. Through use...
Chemotaxis of dendritic cells (DCs) and monocytes is a key step in the initiation an adequate immune response. Formyl peptide receptor (FPR) FPR-like (FPRL)1, two G protein–coupled receptors belonging to FPR family, play essential role host defense mechanisms against bacterial infection regulation inflammatory reactions. FPRL2, third member this structural family chemoattractant receptors, characterized by its specific expression on DCs. Here, we present isolation from spleen extract...
Macrophages derive from multiple sources of hematopoietic progenitors. Most macrophages require colony-stimulating factor 1 receptor (CSF1R), but some persist in the absence CSF1R. Here, we analyzed
Abstract Background Two decades ago, the fish‐specific monoclonal antibody 4C4 was found to be highly reactive zebrafish microglia, macrophages of central nervous system. This has resulted in being widely used, combination with available fluorescent transgenic reporters identify and isolate microglia. However, target protein remains unidentified, which represents a major caveat. In addition, whether expression pattern is strictly restricted microglial cells never been investigated. Results...
Chemokines mediate their biological activity through activation of G protein coupled receptors, but most chemokines, including RANTES, are also able to bind glycosaminoglycans (GAGs). Here, we have investigated, by site-directed mutagenesis and chemical acetylation, the role RANTES basic residues in interaction with GAGs using surface plasmon resonance kinetic analysis. Our results indicate that (i) exhibited selectivity binding highest affinity (Kd = 32.1 nM) for heparin, (ii) uses side...
Abstract Chemerin is a potent chemotactic factor that was identified recently as the ligand of ChemR23, G protein-coupled receptor expressed by mononuclear phagocytes, dendritic cells (DCs), and NK cells. synthesized secreted precursor, prochemerin, which poorly active on ChemR23. However, prochemerin can be converted rapidly into full ChemR23 agonist proteolytic removal carboxy-terminal peptide. This maturation step mediated neutrophil-derived serine proteases elastase cathepsin G. In...
Microglial research has been constrained by a rolling series of dichotomies such as "resting versus activated" or "M1 M2". This Manichean perspective good bad microglia is in sharp contrast with the wide repertoire microglial functions, exerted from development to aging and diseases, brought light recent years. New designations continuously arising attempt describe different states transcriptomics other readouts may thus easily lead misleading, although unintentional, coupling categories...
Chemerin is a multifunctional protein initially characterized in our laboratory as chemoattractant factor for leukocyte populations. Its main functional receptor CMKLR1. We identified previously chemerin an anti-tumoral inhibiting the vascularization of tumor grafts. show here that overexpression bioactive mice results reduction density retinal vascular network during its development and adults. did not affect sprouting post-natal network, but rather promoted endothelial cell apoptosis...
Abstract GPR85/SREB2 is an exceptionally well-conserved orphan 7TM receptor with unclear biological attributes. In this study, we used several zebrafish genetic models to investigate Gpr85 properties and functions throughout development adulthood. We describe that, like in mammals, gpr85 expressed by various neuronal populations adulthood the central nervous system, retina intestine. The overexpression of a fluorochrome-tagged version -expressing neurons provides first vivo evidence that...